Porous polymer scaffold for on-site delivery of stem cells – Protects from oxidative stress and potentiates wound tissue repair

Geesala, Ramasatyaveni; Bar, Nimai; Dhoke, Neha R.; Basak, Pratyay; Das, Amitava

doi:10.1016/j.biomaterials.2015.11.003

Cited by 77 publications

(36 citation statements)

References 42 publications

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“…Exposure to low oxidative stress in vitro (preconditioning with 10 lM H 2 O 2 ) before transplantation at the wound site was hypothesized to increase the proliferation of BMSCs and HSPCs. Thus, we utilized a murine model of excisional splinting wound as described earlier (9) wherein wounds heal through granulation and reepithelialization, a process similar to that which occurs in humans (43). Mice transplanted with preconditioned BMSCs and HSPCs showed an accelerated wound closure postsurgery day 7 as compared with mice transplanted with naive BMSCs and HSPCs only (Fig.…”

Section: Low Oxidative Stress Preconditioned Bmscs and Hspcs Enhance mentioning

confidence: 99%

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Low Oxidative Stress-Mediated ProliferationViaJNK-FOXO3a-Catalase Signaling in Transplanted Adult Stem Cells Promotes Wound Tissue Regeneration

Dhoke

Geesala

Das

2018

Antioxidants & Redox Signaling

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BMSCs and HSPCs depicted an increased growth rate and colony formation, in the presence of 10 μM but not 100 μM concentration of HO, an effect that was perturbed by Vit. C. Mechanistically, JNK activation-FOXO3a nuclear translocation and binding of FOXO3a to catalase promoter at 10 μM HO led to an increased expression and activity of anti-oxidant gene, catalase. This was followed by an increased proliferative phenotype via the AKT-dependent pathway that was perturbed in the presence of catalase-inhibitor, 3-aminotriazole due to an increased ROS-mediated inactivation of AKT. Preclinically, 10 μM HO-mediated preconditioning of BMSCs/HSPCs transplantation accelerated wound closure, enhanced catalase expression, and decreased ROS levels at the wound site. Transplantation of male donor cells into female recipient mice or GFP-labeled BMSCs or HSPCs depicted an increased engraftment and proliferation in preconditioned cell transplanted groups as compared with the wound control. Wound healing occurred via keratinocyte generation and vascularization in preconditioned BMSCs, whereas only neo-vascularization occurred in the preconditioned HSPCs transplanted groups. Innovation and Conclusion: Our study suggests a distinct role of catalase that protects BMSCs and HSPCs from low ROS and promotes proliferation. Transplantation of preconditioned stem cells enhanced wound tissue regeneration with a better antioxidant defense mechanism-as a therapeutic approach in stem cell transplantation-mediated tissue regeneration. Antioxid. Redox Signal. 00, 000-000.

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Section: Low Oxidative Stress Preconditioned Bmscs and Hspcs Enhance mentioning

confidence: 99%

“…Transplantation of bone marrow-derived naive or preconditioned BMSCs and HSPCs was performed by injecting intradermally (id. ; 0.7 • 10 6 cells) and on the wound surface (0.3 • 10 6 cells) as previously described (9). A silicone splint was placed so that the wound was centered within the splint.…”

Section: Bmsc or Hspc Transplantation-mediated Wound Healingmentioning

confidence: 99%

Low Oxidative Stress-Mediated ProliferationViaJNK-FOXO3a-Catalase Signaling in Transplanted Adult Stem Cells Promotes Wound Tissue Regeneration

Dhoke

Geesala

Das

2018

Antioxidants & Redox Signaling

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“…Increased engraftment was seen by expression of Sca-1+Lin-CD90+CD133+. Improved neovascularization was also evident, with increased expression of PECAM, VEGFR3, neuropilin 2, and Tie2 [ 73 ]. In the work of Dong et al, an injectable hydrogel was prepared using a thermoresponsive hyperbranched copolymer of poly(ethylene glycol) methyl ether methacrylate- co -2-(2-methoxyethoxy) ethyl methacrylate- co -poly(ethylene glycol) diacrylate (PEGMEMA-MEO 2 MA-PEGDA).…”

Section: Polymer-based Biomaterials For Stem Cell Delivery For Regmentioning

confidence: 99%

Stem Cells and Engineered Scaffolds for Regenerative Wound Healing

Dash

Lin

et al. 2018

Bioengineering

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The normal wound healing process involves a well-organized cascade of biological pathways and any failure in this process leads to wounds becoming chronic. Non-healing wounds are a burden on healthcare systems and set to increase with aging population and growing incidences of obesity and diabetes. Stem cell-based therapies have the potential to heal chronic wounds but have so far seen little success in the clinic. Current research has been focused on using polymeric biomaterial systems that can act as a niche for these stem cells to improve their survival and paracrine activity that would eventually promote wound healing. Furthermore, different modification strategies have been developed to improve stem cell survival and differentiation, ultimately promoting regenerative wound healing. This review focuses on advanced polymeric scaffolds that have been used to deliver stem cells and have been tested for their efficiency in preclinical animal models of wounds.

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“…In this research, cardiac fibrosis increased dramatically after PAB but reversed by BMSC injection. The expression of MMP 9 upregulated after BMSCs injection, which may not only affect the migration but also reconstruct the extracellular matrix [36]. The angiogenic factor interacts with VEGF since the embryo periods have a positive feedback linking to revascularization.…”

Section: Discussionmentioning

confidence: 99%

The therapeutic impact of human neonatal BMSC in a right ventricular pressure overload model in mice

Liufu

Shi

et al. 2020

Stem Cell Res Ther

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Objective: To determine the impact of donor age on the therapeutic effect of bone marrow-derived mesenchymal stem cells (BMSCs) in treating adverse remodeling as the result of right ventricle (RV) pressure overload. Methods: BMSCs were isolated from neonatal (< 1 month), infant (1 month to 1 year), and young children (1 year to 5 years) and were compared in their migration potential, surface marker expression, VEGF secretion, and matrix metalloprotein (MMP) 9 expression. Four-week-old male C57 mice underwent pulmonary artery banding and randomized to treatment and untreated control groups. During the surgery, BMSCs were administered to the mice by intramyocardial injection into the RV free wall. Four weeks later, RV function and tissue were analyzed by echocardiography, histology, and quantitative real-time polymerase chain reaction.Results: Human neonatal BMSCs demonstrated the greatest migration capacity and secretion of vascular endothelial growth factor but no difference in expression of surface markers. Neonate BMSCs administration resulted in increasing expression of VEGF, a significant reduction in RV wall thickness, and internal diameter in mice after PA banding. These beneficial effects were probably associated with paracrine secretion as no cardiomyocyte transdifferentiation was observed.Conclusions: Human BMSCs from different age groups have different characteristics, and the youngest BMSCs may favorably impact the application of stem cell-based therapy to alleviate adverse RV remodeling induced by pressure overload.

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Porous polymer scaffold for on-site delivery of stem cells – Protects from oxidative stress and potentiates wound tissue repair

Cited by 77 publications

References 42 publications

Low Oxidative Stress-Mediated ProliferationViaJNK-FOXO3a-Catalase Signaling in Transplanted Adult Stem Cells Promotes Wound Tissue Regeneration

Low Oxidative Stress-Mediated ProliferationViaJNK-FOXO3a-Catalase Signaling in Transplanted Adult Stem Cells Promotes Wound Tissue Regeneration

Stem Cells and Engineered Scaffolds for Regenerative Wound Healing

The therapeutic impact of human neonatal BMSC in a right ventricular pressure overload model in mice

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