Porous Molybdenum Carbide Nanostructured Catalyst toward Highly Sensitive Biomimetic Sensing of H₂O₂

Abstract

There are great challenges to fabricate a highly selective and sensitive enzyme‐free biomimetic sensor. Herein for the first time a unique nanostructure of porous molybdenum carbide impregnated in N‐doped carbon (p‐Mo2C/NC) is synthesized by using SiO2 nanocrystals‐templating method and is further used as an enzyme‐free electrochemical biosensor toward highly selective, sensitive detection of H2O2, of which the limit of detection, dynamic detection range and sensitivity accomplish as 0.22 μM, 0.05–4.5 mM and 577.14 μA mM−1 cm−2, respectively, and are much superior to the non‐porous molybdenum carbide impregnated in N‐doped carbon (Mo2C/NC). The sensor is also used to monitor H2O2 released from A549 living cells. This work holds a great promise to be used to monitor the presence of H2O2 in biological research while offering an important knowledge to design a highly selective and sensitive biomimetic sensor by synthesizing a porous catalyst to greatly improve the reaction surface area rather than conventionally only relying on dispersing the catalyst material into porous carbon substrate.

Boosting the Dynamic Range for Electrochemical Sensing of Hydrogen Peroxide by Enhanced Integration of Pd Nanoparticles in 3D Porous Si Framework

“…The detection limits for MACE‐SiNW@PdNP and 3D‐pSi@PdNP electrodes are evaluated by amperometric response at −0.4 V as 1.0 μM and 2.0 μM (with S/N=3) respectively (Figure S2). Compared with some H 2 O 2 electrochemical sensors reported previously (as shown in Table 1), 3D‐pSi@PdNP has broader linear range of detection, comparable sensitivity and lower detection limit [52, 54–57]. From the above results, 3D‐pSi@PdNP seems especially suitable for those scenarios in which large dynamic range of detection is favourable.…”

Single-Atom Iron Anchored on 2-D Graphene Carbon to Realize Bridge-Adsorption of O–O as Biomimetic Enzyme for Remarkably Sensitive Electrochemical Detection of H2O2

“…Hydrogen peroxide (H 2 O 2 ) as a cellular metabolic biomarker is an important typical reactive oxygen species (ROS) in biomedical diagnostics. Its abnormal concentration could break the balance of normal physiological conditions resulting in oxidative stress for different fatal diseases 2 including diabetes, neurodegeneration, Alzheimer's disease, Parkinson's disease, myocardial infarction, and cancer. 3 Therefore, realtime monitoring H 2 O 2 released from living cells is important in clinic diagnosis and bioscience research.…”

“…Various methods have been developed to detect H 2 O 2 including chemiluminescence, fluorescence, photoelectrochemical sensing, colorimetry, electrochemical sensing, and so on. 2 Electrochemical sensors are very attractive due to their advantages of fast response, high sensitivity, low cost, miniaturization, and portability. H 2 O 2 can be electrochemically detected by its reduction under catalysis of peroxidase.…”

“…Therefore, real-time monitoring H 2 O 2 released from living cells is important in clinic diagnosis and bioscience research. Various methods have been developed to detect H 2 O 2 including chemiluminescence, fluorescence, photo­electrochemical sensing, colorimetry, electrochemical sensing, and so on . Electrochemical sensors are very attractive due to their advantages of fast response, high sensitivity, low cost, miniaturization, and portability.…”

Enzyme‐free H2O2 Sensing at ZnO–Graphene Oxide‐modified Glassy Carbon Electrode

“…The detection of hydrogen peroxide (H 2 O 2 ) is of great importance, because it is an essential substrate in textile, mining, clinical, pharmaceutical, and food industries and also an end product of different enzymatic reactions in humans. [1][2][3][4] Numerous analytical methods such as spectrophotometric, 5 titrimetric, 6 chromatographic, 7 colorimetric, 8 and electrochemical techniques [9][10][11] have been employed for monitoring and detection of H 2 O 2 . Among them, the enzyme-free electrochemical method is of particular interest because of its low cost, ease of operation, and in situ quantification.…”