Porous microscaffolds for 3D culture of dental pulp mesenchymal stem cells

Bhuptani, Ronak S.; Patravale, Vandana

doi:10.1016/j.ijpharm.2016.10.040

Cited by 20 publications

(20 citation statements)

References 29 publications

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“…In two case reports [58, 59], a periapical inflammatory lesion with bony defect was filled with a combination of PRF and HA bone graft crystals, and the authors found that HA was replaced by new bone radiographically. HA can also be fabricated into porous scaffolds, which are conducive to cell attachment, migration, and differentiation [83, 84]. An RCT [66] proved that the addition of a porous HA graft to PRF enhanced the percentage of bone fill.…”

Section: Effect Of Prf On Human Oral and Maxillofacial Bone Regenementioning

confidence: 99%

Platelet-Rich Fibrin as a Bone Graft Material in Oral and Maxillofacial Bone Regeneration: Classification and Summary for Better Application

Liu

Sun

et al. 2019

BioMed Research International

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Platelet-rich fibrin (PRF) is an autologous platelet concentrate that consists of cytokines, platelets, leukocytes, and circulating stem cells. It has been considered to be effective in bone regeneration and is mainly used for oral and maxillofacial bone. Although currently the use of PRF is thought to support alveolar ridge preservation, there is a lack of evidence regarding the application of PRF in osteogenesis. In this paper, we will provide examples of PRF application, and we will also summarize different measures to improve the properties of PRF for achieving better osteogenesis. The effect of PRF as a bone graft material on osteogenesis based on laboratory investigations, animal tests, and clinical evaluations is first reviewed here. In vitro, PRF was able to stimulate cell proliferation, differentiation, migration, mineralization, and osteogenesis-related gene expression. Preclinical and clinical trials suggested that PRF alone may have a limited effect. To enlighten researchers, modified PRF graft materials are further reviewed, including PRF combined with other bone graft materials, PRF combined with drugs, and a new-type PRF. Finally, we will summarize the common shortcomings in the application of PRF that probably lead to application failure. Future scientists should avoid or solve these problems to achieve better regeneration.

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Section: Effect Of Prf On Human Oral and Maxillofacial Bone Regenementioning

confidence: 99%

Platelet-Rich Fibrin as a Bone Graft Material in Oral and Maxillofacial Bone Regeneration: Classification and Summary for Better Application

Liu

Sun

et al. 2019

BioMed Research International

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“…37 In addition, the small pore size (1~5 μm) on the closed side seemed to be advantageous for DPSCs mineralization, while another study showed that porous PLGA microscaffolds with pore diameters of 10~30 μm supported the tri-lineage differentiation potential of DPSCs. 38 Growing evidence shows that cytoskeleton change is essential for the cell differentiation, although it may vary depending on differ- The YAP/TAZ pathway is a transcriptional co-activator of the Hippo pathway, which has been implied as a key mediator of mech- which may contribute to the enhanced osteogenic differentiation observed on the closed side. In our hands, enhanced nuclear localization of YAP was consistent with higher F-actin alignment, larger spreading area and higher AR in DPSCs.…”

Section: Discussionmentioning

confidence: 99%

Topographic cues of a novel bilayered scaffold modulate dental pulp stem cells differentiation by regulating YAP signalling through cytoskeleton adjustments

Montoya

Orrego

et al. 2019

Cell Proliferation

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Objectives Topographic cues can modulate morphology and differentiation of mesenchymal stem cells. This study aimed to determine how topographic cues of a novel bilayered poly (lactic‐co‐glycolic acid) (PLGA) scaffold affect osteogenic/odontogenic differentiation of dental pulp stem cells (DPSCs). Methods The surface morphology of the scaffolds was visualized by scanning electron microscope, and the surface roughness was measured by profilometry. DPSCs were cultured on each side of the scaffolds. Cell morphology, expression of Yes‐associated protein (YAP) and osteogenic/odontogenic differentiation were analysed by immunohistochemistry, real‐time polymerase chain reaction, and Alizarin Red S staining. In addition, cytochalasin D (CytoD), an F‐actin disruptor, was used to examine the effects of F‐actin on intracellular YAP localisation. Verteporfin, a YAP transcriptional inhibitor, was used to explore the effects of YAP signalling on osteogenic/odontogenic differentiation of DPSCs. Results The closed side of our scaffold showed smaller pores and less roughness than the open side. On the closed side, DPSCs exhibited enhanced F‐actin stress fibre alignment, larger spreading area, more elongated appearance, predominant nuclear YAP localization and spontaneous osteogenic differentiation. Inhibition of F‐actin alignments was correlated with nuclear YAP exclusion of DPSCs. Verteporfin restricted YAP localisation to the cytoplasm, down‐regulated expression of early osteogenic/odontogenic markers and inhibited mineralization of DPSCs cultures. Conclusions The surface topographic cues changed F‐actin alignment and morphology of DPSCs, which in turn regulated YAP signalling to control osteogenic/odontogenic differentiation.

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“…Giuliani et al used the Micro-CT as an effective tool to observe the proliferation rate of different cells on the PLGA scaffolds (Giuliani et al, 2014). The porous PLGA microscaffolds have been proved to enhance the adhesion of DPSCs, meanwhile maintaining the viability, stemness, and plasticity of the cultured dental pulp mesenchymal stem cells (Bhuptani and Patravale, 2016). And the scaffold morphology was also confirmed to influence the long-term kinetics of bone regeneration (Giuliani et al, 2016).…”

Section: Applications Of Somatic Stem Cells In Oral and Maxillofacialmentioning

confidence: 99%

Application of Stem Cells in Oral Disease Therapy: Progresses and Perspectives

Yang¹,

Qiu²,

Zhou³

et al. 2017

Front. Physiol.

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Stem cells are undifferentiated and pluripotent cells that can differentiate into specialized cells with a more specific function. Stem cell therapies become preferred methods for the treatment of multiple diseases. Oral and maxillofacial defect is one kind of the diseases that could be most possibly cured by stem cell therapies. Here we discussed oral diseases, oral adult stem cells, iPS cells, and the progresses/challenges/perspectives of application of stem cells for oral disease treatment.

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Porous microscaffolds for 3D culture of dental pulp mesenchymal stem cells

Cited by 20 publications

References 29 publications

Platelet-Rich Fibrin as a Bone Graft Material in Oral and Maxillofacial Bone Regeneration: Classification and Summary for Better Application

Platelet-Rich Fibrin as a Bone Graft Material in Oral and Maxillofacial Bone Regeneration: Classification and Summary for Better Application

Topographic cues of a novel bilayered scaffold modulate dental pulp stem cells differentiation by regulating YAP signalling through cytoskeleton adjustments

Application of Stem Cells in Oral Disease Therapy: Progresses and Perspectives

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