Porous gelatin microspheres for controlled drug delivery with high hemostatic efficacy

Cao, Shuang; Li, Lin; Du, Yumin; Gan, Jufen; Wang, Jing; Wang, Tao; Liu, Ying; Liu, Wei; Zhou, Yejin; Gào, Xīn; Liu, Tielong

doi:10.1016/j.colsurfb.2021.112013

Cited by 22 publications

(11 citation statements)

References 31 publications

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“…In liver bleeding experiments, the hemostatic time decreased from ≈40 s to less than 10 s thanks to an increased possible loading of keratins from ≈1.15 to ≈28.67 mg. Compared with the reported common protein hemostatic agents that used to stop rat liver puncture bleedings, [ 19,33,38–47 ] our redesigned soluble α ‐helical keratin at low protein loading (≈1.146 mg) exhibited a comparable hemostatic efficiency to short peptides at the similar dosage and shorter hemostatic time than previous keratins, silk, gelatin, and mussel hemostats at the dosage from 10 to 200 mg (Table S5, Supporting Information). The soluble α ‐helical keratin films showed better hemostatic performance than other existing protein‐based hemostats when the keratin loading increased to ≈28.650 mg (Figure 6G), indicating that the redesigned soluble α ‐helical keratin is a promising candidate for clinical hemostatic applications.…”

Section: Resultsmentioning

confidence: 99%

Rational Design of High‐Performance Keratin‐Based Hemostatic Agents

Wang

Zhang

et al. 2022

Adv Healthcare Materials

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Keratins are considered ideal candidates as hemostatic agents, but the development lags far behind their potentials due to the poorly understood hemostatic mechanism and structure-function relations, owing to the composition complexity in protein extracts. Here, it is shown that by using a recombinant synthesis approach, individual types of keratins can be expressed and used for mechanism investigation and further high-performance keratin hemostatic agent design. In the comparative evaluation of full-length, rod-domain, and helical segment keratins, the 𝜶-helical contents in the sequences are identified to be directly proportional to keratins' hemostatic activities, and Tyr, Phe, and Gln residues at the N-termini of 𝜶-helices in keratins are crucial in fibrinopeptide release and fibrin polymerization. A feasible route to significantly enhance the hemostatic efficiency of helical keratins by mutating Cys to Ser in the sequences for enhanced water wettability through soluble expression is then further presented. These results provide a rational strategy to design high-efficiency keratin hemostatic agents with superior performance over clinically used gelatin sponge in multiple animal models.

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Section: Resultsmentioning

confidence: 99%

Rational Design of High‐Performance Keratin‐Based Hemostatic Agents

Wang

Zhang

et al. 2022

Adv Healthcare Materials

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“…Gelatin is a widely used natural biopolymer in regenerative medicine and tissue engineering [ 123 ]. Similar to the natural biopolymers discussed above, gelatin has been utilized to deliver various types of drugs including anti-fungal/anti-yeast drugs [ 124 ], anti-inflammatory agents [ 125 , 126 , 127 ], antibiotics [ 128 , 129 , 130 ], and chemotherapeutics [ 131 , 132 , 133 , 134 ]. Other factors such as probiotics [ 135 ], vitamins [ 136 ], peptides [ 137 ], and ions [ 138 , 139 ] have been delivered using gelatin.…”

Section: Natural Biopolymer Scaffolds For Therapeutic Ev Deliverymentioning

confidence: 99%

Biomaterials and Extracellular Vesicle Delivery: Current Status, Applications and Challenges

Leung

Shirazi

Cooper

et al. 2022

Cells

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In this review, we will discuss the current status of extracellular vesicle (EV) delivery via biopolymeric scaffolds for therapeutic applications and the challenges associated with the development of these functionalized scaffolds. EVs are cell-derived membranous structures and are involved in many physiological processes. Naïve and engineered EVs have much therapeutic potential, but proper delivery systems are required to prevent non-specific and off-target effects. Targeted and site-specific delivery using polymeric scaffolds can address these limitations. EV delivery with scaffolds has shown improvements in tissue remodeling, wound healing, bone healing, immunomodulation, and vascular performance. Thus, EV delivery via biopolymeric scaffolds is becoming an increasingly popular approach to tissue engineering. Although there are many types of natural and synthetic biopolymers, the overarching goal for many tissue engineers is to utilize biopolymers to restore defects and function as well as support host regeneration. Functionalizing biopolymers by incorporating EVs works toward this goal. Throughout this review, we will characterize extracellular vesicles, examine various biopolymers as a vehicle for EV delivery for therapeutic purposes, potential mechanisms by which EVs exert their effects, EV delivery for tissue repair and immunomodulation, and the challenges associated with the use of EVs in scaffolds.

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“…Gelatin, derived from collagen hydrolysis, − can be used as a hemostatic agent as it is able to activate platelets and induce platelet aggregation . Currently, a variety of absorbable hemostatic materials based on gelatin or gelatin composites are available, such as three-dimensional nanofiber sponges, porous microspheres, and shear-thinning hydrogel . However, these hemostatic materials still have some limitations.…”

Section: Introductionmentioning

confidence: 99%

Shape-Recoverable Macroporous Nanocomposite Hydrogels Created via Ice Templating Polymerization for Noncompressible Wound Hemorrhage

Teng

Xia

Qian

et al. 2022

ACS Biomater. Sci. Eng.

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Uncontrolled hemorrhage resulting from severe trauma or surgical operations remains a challenge. It is highly important to develop functional materials to treat noncompressible wound bleeding. In this work, a shape-recoverable macroporous nanocomposite hydrogel was facilely created through ice templating polymerization. The covalently cross-linked gelatin networks provide a robust framework, while the Laponite nanoclay disperses into the three-dimensional matrix, enabling mechanical reinforcement and hemostatic functions. The resultant macroporous nanocomposite hydrogel possesses an inherent interconnected macroporous structure and rapid deformation recovery. In vitro assessments indicate that the hydrogel displays good cytocompatibility and a low hemolysis ratio. The hydrogel shows a higher coagulation potential and more erythrocyte adhesion compared to the commercial gauze and gelatin sponge. The noncompressible liver hemorrhage models also confirm its promising hemostasis performance. This strategy of combining a nano-enabled solution with ice templating polymerization displays great potential to develop appealing absorbable macroporous biomaterials for rapid hemostasis.

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Porous gelatin microspheres for controlled drug delivery with high hemostatic efficacy

Cited by 22 publications

References 31 publications

Rational Design of High‐Performance Keratin‐Based Hemostatic Agents

Rational Design of High‐Performance Keratin‐Based Hemostatic Agents

Biomaterials and Extracellular Vesicle Delivery: Current Status, Applications and Challenges

Shape-Recoverable Macroporous Nanocomposite Hydrogels Created via Ice Templating Polymerization for Noncompressible Wound Hemorrhage

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