Porous chitosan microspheres as microcarriers for 3D cell culture

Huang, Lixia; Xiao, Lin; Poudel, Abishek Jung; Li, Jixiang; Zhou, Ping; Gauthier, Mario; Liu, Haiqing; Wu, Zhihong; Yang, Guang

doi:10.1016/j.carbpol.2018.09.021

Cited by 76 publications

(54 citation statements)

References 54 publications

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“…The 2D conventional cell culture systems performed on typical tissue culture plates, in which roughly dose experiments are performed for in vitro imaging and drug applications. But, the 2D conventional cell culture systems has been questioned because of potential changes in morphology and gene expression, often resulting in distortions in cell behavior and biological functions of cultured cells as compared with cells in natural organisms and tissues (Fang & Eglen, ; Huang et al., ; Jin et al., ). As for the animal model, the animals model has many uncontrollable factors such as endogenous growth factors and lack of an immune system (Jin et al., ).To better get the cell morphology, behavior and functions, the concept of 3D cell culture was proposed, that is cell growth in a 3D environment with sufficient and multidirectional cell–cell interactions mimicking the in vivo architecture of natural organs and tissues (Achilli, Meyer, & Morgan, ; Kehr, ).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and Antiproliferative Activities of Cyanidin‐3‐O‐Glucoside (C3G) Liposome in Caco‐2 Cells Cultivated in 2D and 3D Cell Culture Models

Liang

Qian

Guan

et al. 2019

Journal of Food Science

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The aim of this study was to obtain adequate and detailed information about the antioxidant and antiproliferative activities of C3G and C3G liposomes in different cell culture models. The Caco‐2 cells were cultured in 2D and 3D cell culture models, the H2O2 was used to construct the cell damage model and then the cells treated with C3G and C3G liposomes. The antioxidant activity and antiproliferative activities of C3G liposomes on Caco‐2 cells were investigated. We observed the morphology of cells and measured the cell viability, the activity of glutathione (GSH), superoxide dismutase (SOD), total antioxidant capacity (T‐AOC), and the content of malondialdehyde (MDA) in Caco‐2 cells treated with H2O2, C3G, and C3G liposomes. The results showed that the Caco‐2 cells cultured in the 3D culture model formed a 3D structure and tight spheroids and showed the increase of cell activity in 3D cell culture model, compared with the 2D cell culture model. The C3G and C3G liposomes can enhance the activities of GSH, SOD, and T‐AOC but decrease the MDA content after H2O2 treatment, while the changes were different in 2D and 3D cells culture models. This study revealed that the results obtained from the 2D cell model may be inaccurate compared with the results obtained from the 3D cell model. Practical Application The results of this study showed that the results obtained from the 2D cell model may be inaccurate compared with the results obtained from the 3D cell model. Our work provides a method for evaluating antioxidant activity of C3G liposomes in different cell models and provided certain theoretical basis for the follow‐up research.

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Section: Introductionmentioning

confidence: 99%

Antioxidant and Antiproliferative Activities of Cyanidin‐3‐O‐Glucoside (C3G) Liposome in Caco‐2 Cells Cultivated in 2D and 3D Cell Culture Models

Liang

Qian

Guan

et al. 2019

Journal of Food Science

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“…On the other hand, the emergence of recombinant collagen extracted from plants has important advantages as a scaffold in its own right or if used to functionalize the surfaces of the materials above. Furthermore, these materials have been modified, either as microspheres or bulk, to possess the porosity necessary for diffusion of nutrients through dynamic or static bioreactors (Oh et al, 2009;Wu et al, 2011;García Cruz et al, 2012;Varley et al, 2017;Huang et al, 2018;Specht et al, 2018). In order to scale an animal-free product with similarities to that of native animal tissue, the need for fetal bovine serum, costeffective engineering processes, antibiotic dependence, scaffold development, and cell line(immortalized vs. primary and cell co-culture) needs to be addressed (Specht et al, 2018;Stephens et al, 2018;Lynch and Pierrehumbert, 2019).…”

Section: Resultsmentioning

confidence: 99%

Scaffolds for 3D Cell Culture and Cellular Agriculture Applications Derived From Non-animal Sources

Campuzano

Pelling

2019

Front. Sustain. Food Syst.

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For decades, two-dimensional cell culture has been regarded as a major tool in cellular and molecular biology due to its simplicity, reproducibility and reliable nature. However, it is now recognized that 2D cell culture underrepresents the in vivo environment of living cells. The development and use of 3D scaffolds and biomaterials provide researchers an ability to more closely mimic the in vivo environment. However, many biomaterials are of animal origin, leading to variability, environmental and ethical concerns. Here we present three animal-free scaffolds: decellularized plant tissue, chitin/chitosan and recombinant collagen. Decellularized plant tissue provides a wide array of structures with varying biochemical, topographical and mechanical properties; chitin/chitosan-based scaffolds have shown synergistic bactericidal effects and improved cell-matrix interaction; and lastly, recombinant collagen has the potential to closely resemble native tissue, as opposed to the other two. These benefits, alongside potential scalability and tunability, open the door to applications beyond the biomedical realm, such as innovations in cellular agriculture and future food technologies.

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“…The achieved biomimetic surface showed enhanced bioactivity and dispersion ability in the cell medium [53]. By using a process combining micro-emulsification and thermally induced phase separation (TIPS), we prepared chitosan microspheres with a highly porous structure as microcarriers for 3D cell culture [54]. Besides, the water-in-oil (W/O) emulsification process was combined with the freeze-drying process to prepare aerogel microspheres from the hybrid of poly(vinyl alcohol) (PVA) and cellulose nanofibril (CNF) [55]; while the combination of the oil-in-water (O/W) single-emulsion method and porogen leaching-phase separation process was applied to prepare PLGA porous microspheres with a size of approximately 50 µm for cell microcarrier application [56].…”

Section: Fabrication Techniques For Three-dimensional (3d) Cell Micromentioning

confidence: 99%

“…Micro-emulsification and thermally induced phase separation (TIPS) 150 [54] Combination of the water-in-oil (W/O) emulsification process and the freeze-drying process…”

Section: Non-porous Microspherementioning

confidence: 99%

Biopolymer-Based Microcarriers for Three-Dimensional Cell Culture and Engineered Tissue Formation

Huang

Abdalla

Xiao

et al. 2020

IJMS

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The concept of three-dimensional (3D) cell culture has been proposed to maintain cellular morphology and function as in vivo. Among different approaches for 3D cell culture, microcarrier technology provides a promising tool for cell adhesion, proliferation, and cellular interactions in 3D space mimicking the in vivo microenvironment. In particular, microcarriers based on biopolymers have been widely investigated because of their superior biocompatibility and biodegradability. Moreover, through bottom-up assembly, microcarriers have opened a bright door for fabricating engineered tissues, which is one of the cutting-edge topics in tissue engineering and regeneration medicine. This review takes an in-depth look into the recent advancements of microcarriers based on biopolymers—especially polysaccharides such as chitosan, chitin, cellulose, hyaluronic acid, alginate, and laminarin—for 3D cell culture and the fabrication of engineered tissues based on them. The current limitations and potential strategies were also discussed to shed some light on future directions.

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Porous chitosan microspheres as microcarriers for 3D cell culture

Cited by 76 publications

References 54 publications

Antioxidant and Antiproliferative Activities of Cyanidin‐3‐O‐Glucoside (C3G) Liposome in Caco‐2 Cells Cultivated in 2D and 3D Cell Culture Models

Antioxidant and Antiproliferative Activities of Cyanidin‐3‐O‐Glucoside (C3G) Liposome in Caco‐2 Cells Cultivated in 2D and 3D Cell Culture Models

Scaffolds for 3D Cell Culture and Cellular Agriculture Applications Derived From Non-animal Sources

Biopolymer-Based Microcarriers for Three-Dimensional Cell Culture and Engineered Tissue Formation

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