Porous chitosan-hyaluronic acid scaffolds as a mimic of glioblastoma microenvironment ECM

Florczyk, Stephen J.; Wang, Kui; Jana, Soumen; Wood, David L.; Sytsma, Samara K.; Sham, Jonathan G.; Kievit, Forrest M.; Zhang, Miqin

doi:10.1016/j.biomaterials.2013.09.034

Cited by 188 publications

(160 citation statements)

References 65 publications

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“…Among the ECM components, an important role has been demonstrated for HA, able to regulate the glioma cell phenotype [231] or to increase stem-like properties of glioblastoma cells [232]. For this reason, hydrogels containing HA have been proposed as a scaffold for neuronal tumors [233].…”

Section: D Tumoral Cns Cultures On Scaffoldsmentioning

confidence: 99%

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In Vitro Modeling of Tissue-Specific 3D Microenvironments and Possibile Application to Pediatric Cancer Research

Pizzimenti¹

2014

J. Pediatr. Oncol.

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A large body of evidence indicates that three dimensional (3D) cancer models are superior to two-dimensional (2D) ones in better representing the in vivo phenomena. Indeed, 3D models allow recapitulating in vitro the in vivo features observed in solid tumors (e.g. cell polarity, cell-cell/cell-matrix interactions, biochemical/metabolic gradients, anchorage-independent growth and hypoxia). Moreover, it is well established that the microenvironment plays a fundamental role in regulating tumor development and behavior, including drug resistance. Thus, innovative models able to mimic this complexity represent attractive tools in cancer research. In this review article, we provide a comprehensive review of the application of 3D culture systems in pediatrics' cancer research. In particular, 3D in vitro/ex vivo models of the most common pediatric tumors, such as leukemias, lymphomas and malignancies of the nervous system, will be considered.

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Section: D Tumoral Cns Cultures On Scaffoldsmentioning

confidence: 99%

“…Moreover, several other matrix compositions have been tested with the aim of better reproducing the in vivo microenvironment. Thus, invasion assays have been performed in HA-based hydrogel [230,[233][234][235]]. …”

Section: D Models For Testing Motility/invasion Of Cns Tumorsmentioning

confidence: 99%

In Vitro Modeling of Tissue-Specific 3D Microenvironments and Possibile Application to Pediatric Cancer Research

Pizzimenti¹

2014

J. Pediatr. Oncol.

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“…to promote CD133 overexpression, making it useful for the study of glioma CSCs. 53 Porous HA-chitosan scaffolds have also been used to culture multicellular spheroids composed of glioblastoma 54 and non-small cell lung cancer cells, 55 noting increased spheroid formation, stem-like properties, and invasiveness in both cases. Additionally, non-small cell lung cancer cells revealed more EMT markers as well as exhibited 5-6 times higher resistance to cisplatin and 16-56 times higher resistance to methotrexate compared to conventional 2D cultures.…”

Section: Biomaterials For Csc Growthmentioning

confidence: 99%

The Role of Biomaterials on Cancer Stem Cell Enrichment and Behavior

Ordikhani

Kim

Zustiak

2015

JOM

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The theory of cancer stem cells (CSCs) and their role in cancer metastasis, tumorigenicity and resistance to therapy is slowly shifting the emphasis on the search for cancer cure: more evidence is surfacing that a successful therapy should be geared against this rare cancer cell population. Unfortunately, CSCs are difficult to culture in vitro which severely limits the progress of CSC research. This review gives a brief overview of CSCs and their microenvironment, with particular focus on studies that used in vitro biomaterial-based models and biomaterial/CSC interfaces for the enrichment of CSCs. Biomaterial properties relevant to CSC behaviors are also addressed. While the discussed research field is still in its infancy, it appears that in vitro cancer models that include a biomaterial can support CSC enrichment and this has proved indispensable to the study of their biology as well as the development of novel cancer therapies.

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“…Primary cells from glioma patient tumor material exposed to increasing concentrations of HA responded with rounded morphology and reduced migration, suggesting that HA concentrations may affect glioma cell behavior. [62] Consistently, addition of HA to porous chitosan scaffolds [63] or to artificial hydrogels [64] increased the expression of stem cell markers and VEGF and HIF-1, respectively. However, the finding that increasing matrix stiffness -by adding agarose to a collagen I matrix -blocks glioma invasiveness, [19] suggested that stiffness alone and independent of ligand binding acted as a critical determinant for primary brain tumor cell function.…”

Section: The Impact Of the Embedding Matrix On The Behavior Of The Tumentioning

confidence: 68%

Dissecting brain tumor growth and metastasis in vitro and ex vivo

Grotzer¹,

Neve²,

Baumgärtner³

2016

JCMT

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Local infiltration and distal dissemination of tumor cells hamper efficacy of current treatments against central nervous system (CNS) tumors and greatly influence mortality and therapy-induced longterm morbidity in survivors. A number of in vitro and ex vivo assay systems have been established to better understand the infiltration and metastatic processes, to search for molecules that specifically block tumor cell infiltration and metastatic dissemination and to pre-clinically evaluate their efficaciousness. These systems allow analytical testing of tumor cell viability and motile and invasive capabilities in simplified and well-controlled environments. However, the urgent need for novel anti-metastatic therapies has provided an incentive for the further development of not only classical in vitro methods but also of novel, physiologically more relevant assay systems including organotypic brain slice culture. In this review, using publicly available peer-reviewed primary research and review articles, we provide an overview of a selection of in vitro and ex vivo techniques widely used to study growth and dissemination of primary metastatic brain tumors. Furthermore, we discuss how our steadily increasing knowledge of tumor biology and the tumor microenvironment could be integrated to improve current research methods for metastatic brain tumors. We believe that such rationally improved methods will ultimately increase our understanding of the biology of brain tumors and facilitate the development of more efficacious anti-metastatic treatments. Local infiltration and distal dissemination of tumor cells hamper efficacy of current treatments against central nervous system (CNS) tumors and greatly influence mortality and therapy-induced long-term morbidity in survivors. A number of in vitro and ex vivo assay systems have been established to better understand the infiltration and metastatic processes, to search for molecules that specifically block tumor cell infiltration and metastatic dissemination and to pre-clinically evaluate their efficaciousness. These systems allow analytical testing of tumor cell viability and motile and invasive capabilities in simplified and well-controlled environments. However, the urgent need for novel anti-metastatic therapies has provided an incentive for the further development of not only classical in vitro methods but also of novel, physiologically more relevant assay systems including organotypic brain slice culture. In this review, using publicly available peer-reviewed primary research and review articles, we provide an overview of a selection of in vitro and ex vivo techniques widely used to study growth and dissemination of primary metastatic brain tumors. Furthermore, we discuss how our steadily increasing knowledge of tumor biology and the tumor microenvironment could be integrated to improve current research methods for metastatic brain tumors. We believe that such rationally improved methods will ultimately increase our understanding of the biology of brain tumors ...

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Porous chitosan-hyaluronic acid scaffolds as a mimic of glioblastoma microenvironment ECM

Cited by 188 publications

References 65 publications

In Vitro Modeling of Tissue-Specific 3D Microenvironments and Possibile Application to Pediatric Cancer Research

In Vitro Modeling of Tissue-Specific 3D Microenvironments and Possibile Application to Pediatric Cancer Research

The Role of Biomaterials on Cancer Stem Cell Enrichment and Behavior

Dissecting brain tumor growth and metastasis in vitro and ex vivo

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