Porous chitosan-gelatin scaffold containing plasmid DNA encoding transforming growth factor-β1 for chondrocytes proliferation

Guo, Ting; Zhao, Jianning; Chang, Jianbin; Ding, Zhi; Hong, Hao; Chen, Jiangning; Zhang, Junfeng

doi:10.1016/j.biomaterials.2005.08.015

Cited by 149 publications

(97 citation statements)

References 39 publications

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“…A notable finding of this study was that covalently linking as little as 10 mg of plasmid DNA to a type II collagen-GAG scaffold can result in the prolonged overexpression of a growth factor, thus allowing (nonviral) gene transfer to be safely joined to tissue engineering. Previous work using naked plasmid DNA alone incorporated within scaffolds has also demonstrated elevated levels of encoded protein, but using plasmid loads that were significantly higher (on the order of milligram levels per scaffold) 8,14,25,26 compared to the current study. The significantly lower amounts of plasmid needed in the present study to produce elevated and prolonged IGF-1 expression levels may be explained by lipid-mediated transfection along with the controlled delivery of plasmid, enhancing the conditions for gene uptake.…”

Section: Discussioncontrasting

confidence: 54%

Collagen scaffolds for nonviral IGF-1 gene delivery in articular cartilage tissue engineering

2007

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This study investigated the use of a type II collagenglycosaminoglycan (CG) scaffold as a nonviral gene delivery vehicle for facilitating gene transfer to seeded adult articular chondrocytes to produce an elevated, prolonged and local expression of insulin-like growth factor (IGF)-1 for enhancing cartilage regeneration. Gene-supplemented CG (GSCG) scaffolds were synthesized by two methods: (1) soaking a pre-cross-linked CG scaffold in a plasmid solution followed by a freeze-drying process, and (2) chemically cross-linking the plasmid DNA to the scaffold. Two different plasmid solutions were also compared: (1) naked plasmid IGF-1 alone, and (2) plasmid IGF-1 with a lipid transfection reagent. Plasmid release studies revealed that cross-linking the plasmid to the CG scaffold prevented passive bolus release of plasmid and resulted in vector release controlled by scaffold degradation. In chondrocyte-seeded GSCG scaffolds, prolonged and elevated IGF-1 expression was enhanced by using the cross-linking method of plasmid incorporation along with the addition of the transfection reagent. The sustained level of IGF-1 overexpression resulted in significantly higher amounts of tissue formation, chondrocyte-like cells, GAG accumulation, and type II collagen production, compared to control scaffolds. These findings demonstrate that CG scaffolds can serve as nonviral gene delivery vehicles of microgram amounts of IGF-1 plasmid (o10 mg per scaffold) to provide a locally sustained therapeutic level of overexpressed IGF-1, resulting in enhanced cartilage formation.

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Section: Discussioncontrasting

confidence: 54%

Collagen scaffolds for nonviral IGF-1 gene delivery in articular cartilage tissue engineering

2007

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“…A nonexhaustive overview of the most recent publications, subdivided by application, for either collagen or gelatine alone or in a combination of other biopolymers is summarized in Table 2 which clearly indicates that gelatine has a wider-application range within the field of both soft and hard-tissue engineering. (Spira et al, 2004) skin replacement artificial skin dermis (Harriger et al, 1998) skin tissue engineering Tangsadthakun et al, 2006) artificial skin (Choi et al, 1999;Lee et al, 2003) soft tissue adhesives (McDermott et al, 2004) Surgery hemostatic agent (Cameron, 1978;Browder & Litwin, 1986) plasma expander suture wound dressing and repair (Rao, 1995) skin replacement (artificial skin) nerve repair and conduits blood vessel prostheses (Auger et al, 1998;McGuigan et al, 2006, Amiel et al, 2006 small intestine ( Chiu et al, 2009) liver -chitosan/gelatine scaffold (Jiankang et al, 2007) wound dressing (Tucci & Ricotti, 2001) nerve regenerationchitosan/gelatin scaffolds (Chiono et al, 2008) blod vesels ( Mironov et al, 2005) Orthopaedic born, tendon and ligament repair cartilage reconstruction -collagen (Stone, 1997), composite of collagen type II/chondroitin/hyaluronan (Jančar et al, 2007) articular cartilagecollagen/chitosan (Yan et al, 2010) bone substitutegelatine/hydroxyapatite (Chang et al, 2007) hard tissue regenerationgelatine/hydroxyapatite ( Kim et al, 2005) cartilage (Lien et al, 2010) cartilage defects regenerationchitosan/ gelatine (Guo et al, 2006), ceramic/ gelatine bone substitutegelatine/tricalcium phosphate (Yao et al, 2005) Ophthalmology corneal graft (Lass et al, 1986) vitreous implants artificial tears (Kaufman et al, 1994) tape and retinal reattachment contact lenses eye disease treatment (http.....) ocu...…”

Section: Collagen Vs Gelatine As Biomaterialsmentioning

confidence: 99%

Collagen- vs. Gelatine-Based Biomaterials and Their Biocompatibility: Review and Perspectives

Gorgieva¹,

Kokol²

2011

Biomaterials Applications for Nanomedicine

244

250

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“…Points of F-actin and vinculin colocalization have been shown to be sites where chondrocytes adhere to the articular cartilage ECM (47). Many researchers have chosen to focus on the 3-D study of cell-seeded matrices in an effort to engineer articular cartilage in vitro for implantation in vivo (7,48). For these purposes, we have recently developed a self-assembly process (27).…”

Section: Discussionmentioning

confidence: 99%

Isolation and chondroinduction of a dermis‐isolated, aggrecan‐sensitive subpopulation with high chondrogenic potential

Deng

Athanasiou

2007

Arthritis & Rheumatism

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Objective. To develop a process that yields tissueengineered articular cartilage constructs from skinderived cells.Methods. Dermis-isolated, aggrecan-sensitive (DIAS) cells were isolated using a modified rapid adherence process. The chondrogenic potential was measured by quantitative reverse transcriptase-polymerase chain reaction, enzyme-linked immunosorbent assay, and immunohistochemistry. Filamentous actin (Factin) and vinculin organization was detected using fluorescence microscopy.Results. The rapid adherence process led to a selection of DIAS cells, <10% of the entire population. DIAS cells displayed greater chondroinduction potential, as evidenced by the formation of large numbers of chondrocytic nodules on aggrecan-coated surfaces. In addition, these cells showed higher gene expression and protein production in terms of chondrocytic markers when compared with unpurified dermis cells. Similar patterns of F-actin and vinculin organization were observed between DIAS cells and chondrocytes. Threedimensional constructs from chondroinduced DIAS cells produced greater amounts of cartilage matrix than constructs from the rest of the dermis populations.Conclusion. These findings show a series of steps that work together to form tissue-engineered articular cartilage constructs using DIAS cells. Since skin presents a minimally invasive, relatively abundant cell source for tissue engineering, this study offers evidence of an efficient and stable technique to form cartilage constructs for future cartilage regeneration with autologous cells from skin.

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Porous chitosan-gelatin scaffold containing plasmid DNA encoding transforming growth factor-β1 for chondrocytes proliferation

Cited by 149 publications

References 39 publications

Collagen scaffolds for nonviral IGF-1 gene delivery in articular cartilage tissue engineering

Collagen scaffolds for nonviral IGF-1 gene delivery in articular cartilage tissue engineering

Collagen- vs. Gelatine-Based Biomaterials and Their Biocompatibility: Review and Perspectives

Isolation and chondroinduction of a dermis‐isolated, aggrecan‐sensitive subpopulation with high chondrogenic potential

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