Porous antibody-containing protein microparticles as novel carriers for ELISA

Neumann, Marina M.; Volodkin, Dmitry

doi:10.1039/c9an01888c

Cited by 7 publications

(4 citation statements)

References 24 publications

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“…The vaterite polymorph is a promising carrier for delivering drugs and bioactive agents for clinical applications [ 32 ]. It is of note that vaterite has successfully been utilized to assemble polymer-based structures, such as the layer-by-layer assembled capsules [ 34 ] or polymer beads (i.e., pure protein particles [ 35 , 36 ]). This approach is based on sequential deposition of polymers upon the surface of vaterite crystals, followed by decomposition of the vaterite matrix, and finally, the formulation of a polymer-based network (so-called capsules or particles).…”

Section: Modification Of Various Surfaces With Vaterite Cacomentioning

confidence: 99%

Modification of Surfaces with Vaterite CaCO3 Particles

et al. 2022

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Former studies have demonstrated a strong interest toward the crystallization of CaCO3 polymorphs in solution. Nowadays, CaCO3 crystallization on solid surfaces is extensively being studied using biomolecules as substrates for the control of the growth aiming at various applications of CaCO3. Calcium carbonate exists in an amorphous state, as three anhydrous polymorphs (aragonite, calcite and vaterite), and as two hydrated polymorphs (monohydrocalcite and ikaite). The vaterite polymorph is considered as one of the most attractive forms due to its large surface area, biocompatibility, mesoporous nature, and other features. Based on physical or chemical immobilization approaches, vaterite can be grown directly on solid surfaces using various (bio)molecules, including synthetic polymers, biomacromolecules such as proteins and peptides, carbohydrates, fibers, extracellular matrix components, and even biological cells such as bacteria. Herein, the progress on the modification of solid surfaces by vaterite CaCO3 crystals is reviewed, focusing on main findings and the mechanism of vaterite growth initiated by various substances mentioned above, as well as the discussion of the applications of such modified surfaces.

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Section: Modification Of Various Surfaces With Vaterite Cacomentioning

confidence: 99%

Modification of Surfaces with Vaterite CaCO3 Particles

et al. 2022

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“…Recently, antibody-containing protein microparticles for the ELISA-based detection of human immunoglobulin G were produced by Neumann and Volodkin (2020) [187]. BSA particles can be achieved.…”

Section: Beads Formed From Porous Templatesmentioning

confidence: 99%

“…Recently, antibody-containing protein microparticles for the ELISA-based detection of human immunoglobulin G were produced by Neumann and Volodkin (2020) [187]. BSA and goat anti-human immunoglobulin G were co-synthesised into CaCO 3 crystals, reaching loading efficiencies of up to 70%, followed by cross-linking with glutaraldehyde and dissolution with EDTA.…”

Section: Beads Formed From Porous Templatesmentioning

confidence: 99%

Biopolymer-Based Multilayer Capsules and Beads Made via Templating: Advantages, Hurdles and Perspectives

Vikulina

Campbell

2021

Nanomaterials

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One of the undeniable trends in modern bioengineering and nanotechnology is the use of various biomolecules, primarily of a polymeric nature, for the design and formulation of novel functional materials for controlled and targeted drug delivery, bioimaging and theranostics, tissue engineering, and other bioapplications. Biocompatibility, biodegradability, the possibility of replicating natural cellular microenvironments, and the minimal toxicity typical of biogenic polymers are features that have secured a growing interest in them as the building blocks for biomaterials of the fourth generation. Many recent studies showed the promise of the hard-templating approach for the fabrication of nano- and microparticles utilizing biopolymers. This review covers these studies, bringing together up-to-date knowledge on biopolymer-based multilayer capsules and beads, critically assessing the progress made in this field of research, and outlining the current challenges and perspectives of these architectures. According to the classification of the templates, the review sequentially considers biopolymer structures templated on non-porous particles, porous particles, and crystal drugs. Opportunities for the functionalization of biopolymer-based capsules to tailor them toward specific bioapplications is highlighted in a separate section.

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“…Furthermore, by using a porous particle as a capturing 3D substrate, the kinetics involved in the substrate–analyte interaction is more similar to the binding of two free molecules in the solution, thus reducing the drawbacks connected to the diffusion-limited binding kinetics. Since incubation times cannot be infinite, increased diffusion and binding kinetics coupled with a larger capture surface could also lead to an improved LOD . For this purpose, the integration of dense beads and porous beads as 3D substrates in biomolecular assays turned out to be promising ,, and able to guarantee high-throughput analysis and easy integration into different sensing platforms. − By coupling these properties with high loading capability, a characteristic of the mesoporous structure, it is possible to design a versatile substrate for low-volume biomolecular detection at low concentrations.…”

Section: Introductionmentioning

confidence: 99%

Advanced ELISA-like Biosensing Based on Ultralarge-Pore Silica Microbeads

Calmo

Chiadò

Fiorilli

et al. 2020

ACS Appl. Bio Mater.

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In this work, functionalized porous silica-based materials, widely used in the literature as drug and biomolecule nanocarriers, were innovatively used as an effective three-dimensional (3D) substrate for the development of a specific biomolecular assay showing great versatility in terms of detection performance. One-pot synthesis of ultralarge-pore silica microbeads was optimized to develop an enzyme-linked immunosorbent (ELISA)-like DNA detection assay. Cocondensation synthesis enabled introducing thiol functionalities into the silica framework while preserving both the high specific surface area (560 m 2 /g) and large pore size (17 nm average diameter), which are essential to guaranteeing high loading capability. Indeed, the bead-capturing ability was proved by developing an ELISA-like assay for the detection of short DNA sequences (≈20 bp), both in labeled and label-free configurations. In particular, the suppression of unspecific binding on the bead surface by testing two different blocking agents was a matter of interest. The detection performances were evaluated and compared to the ones obtained by following the same detection protocol on a standard flat surface (two-dimensional, 2D), which is most commonly used for this purpose. The bead-based assay showed a limit of detection two times lower than the flat-surface assay, confirming the promising capturing ability due to the larger active surface area. Furthermore, compared to traditional ELISA, the bead-based assay showed an intrinsic larger dynamic range that can be tailored depending on the final amount of beads used for the colorimetric quantification.

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Porous antibody-containing protein microparticles as novel carriers for ELISA

Abstract: Synthesis of porous antibody-based protein microparticles as novel effective carriers for ELISA.

Cited by 7 publications

References 24 publications

Modification of Surfaces with Vaterite CaCO3 Particles

Modification of Surfaces with Vaterite CaCO3 Particles

Biopolymer-Based Multilayer Capsules and Beads Made via Templating: Advantages, Hurdles and Perspectives

Advanced ELISA-like Biosensing Based on Ultralarge-Pore Silica Microbeads

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