‘Porosome’ discovered nearly 20 years ago provides molecular insights into the kiss‐and‐run mechanism of cell secretion

Jena, Bhanu P.

doi:10.1111/jcmm.12598

Cited by 18 publications

(13 citation statements)

References 53 publications

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“…It could indicate that during embryonic development of the grass snake the content of zymogen granules was partially released to the intercalated ducts. This mode of secretion is connected with the incomplete fusion of the granule and cell membrane within the kiss‐and‐run mechanism, similarly as in humans (Jena, ).…”

Section: Discussionmentioning

confidence: 91%

Development of pancreatic acini in embryos of the grass snake Natrix natrix (Lepidosauria, Serpentes)

Kowalska

Rupik

2019

Journal of Morphology

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This study report about the differentiation of pancreatic acinar tissue in grass snake, Natrix natrix, embryos using light microscopy, transmission electron microscopy, and immuno‐gold labeling. Differentiation of acinar cells in the embryonic pancreas of the grass snake is similar to that of other amniotes. Pancreatic acini occurred for the first time at Stage VIII, which is the midpoint of embryonic development. Two pattern of acinar cell differentiation were observed. The first involved formation of zymogen granules followed by cell migration from ducts. In the second, one zymogen granule was formed at the end of acinar cell differentiation. During embryonic development in the pancreatic acini of N. natrix, five types of zymogen granules were established, which correlated with the degree of their maturation and condensation. Within differentiating acini of the studied species, three types of cells were present: acinar, centroacinar, and endocrine cells. The origin of acinar cells as well as centroacinar cells in the pancreas of the studied species was the pancreatic ducts, which is similar as in other vertebrates. In the differentiating pancreatic acini of N. natrix, intermediate cells were not present. It may be related to the lack of transdifferentiation activity of acinar cells in the studied species. Amylase activity of exocrine pancreas was detected only at the end of embryonic development, which may be related to animal feeding after hatching from external sources that are rich in carbohydrates and presence of digestive enzymes in the egg yolk. Mitotic division of acinar cells was the main mechanism of expansion of acinar tissue during pancreas differentiation in the grass snake embryos.

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Section: Discussionmentioning

confidence: 91%

Development of pancreatic acini in embryos of the grass snake Natrix natrix (Lepidosauria, Serpentes)

Kowalska

Rupik

2019

Journal of Morphology

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“…Nearly 38 trillion cells composing the human body communicate with each other via secreted biomolecules. In 1996, plasma membrane-associated 100-120 nm cup-shaped lipoprotein structures called porosomes were discovered in the exocrine pancreas and subsequently in the endocrine cells and neurons, that enable the secretion of biomolecules from cells (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13).…”

Section: Introductionmentioning

confidence: 99%

Possible Mechanism of Porosome-Mediated Exosome Release

Cho

Formosa

Zhvania

et al. 2022

Preprint

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Supramolecular cup-shaped lipoprotein structures called porosomes embedded in the cell plasma membrane mediate fractional release of intra-vesicular contents from cells during secretion. The presence of porosomes, have been documented in many cell types including neurons, acinar cells of the exocrine pancreas, growth hormone secreting cells of the pituitary, and mouse insulin-secreting beta cell insulinomas called Min6. Electron micrographs of the nerve terminal in rat brain and of Min6 cells, suggest that besides the docking, fusion and content release of neurotransmitter containing synaptic vesicles and insulin containing granules, multi-vesicular bodies and exosome-like vesicles are released at the porosome complex. To test this hypothesis, the porosome-associated calcium transporting ATPase 1 [SPCA1] encoded by the ATP2C1 gene, was knocked-out in Min6 cells using CRISPR, to study glucose-stimulated insulin and exosome release. In agreement with electron micrographs, results from the study demonstrate a loss of both glucose-stimulated insulin and exosome release in the ATP2C1 knockout Min6 cells. These results further confirm the role of the porosome complex in insulin secretion and establishes a new paradigm in porosome-mediated exosome release in beta cells of the endocrine pancreas.

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“…It has been suggested that vesicle swelling is involved in the exocytotic process [1,2,3]. In secretory vesicles, a specific set of ion channels expressed in the membrane has been proposed to contribute to the secretory granule swelling [4,5,6,7,8].…”

Section: Introductionmentioning

confidence: 99%

Gi Protein Modulation of the Potassium Channel TASK-2 Mediates Vesicle Osmotic Swelling to Facilitate the Fusion of Aquaporin-2 Water Channel Containing Vesicles

Centrone

Santo

Nicotera

et al. 2018

Cells

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Vesicle fusion is a fundamental cell biological process similar from yeasts to humans. For secretory vesicles, swelling is considered a step required for the expulsion of intravesicular content. Here this concept is revisited providing evidence that it may instead represent a general mechanism. We report the first example that non-secretory vesicles, committed to insert the Aquaporin-2 water channel into the plasma membrane, swell and this phenomenon is required for fusion to plasma membrane. Through an interdisciplinary approach, using atomic force microscope (AFM), a fluorescence-based assay of vesicle volume changes and NMR spectroscopy to measure water self-diffusion coefficient, we provide evidence that Gi protein modulation of potassium channel TASK-2 localized in AQP2 vesicles, is required for vesicle swelling. Estimated intravesicular K+ concentration in AQP2 vesicles, as measured by inductively coupled plasma mass spectrometry, was 5.3 mM, demonstrating the existence of an inwardly K+ chemical gradient likely generating an osmotic gradient causing vesicle swelling upon TASK-2 gating. Of note, abrogation of K+ gradient significantly impaired fusion between vesicles and plasma membrane. We conclude that vesicle swelling is a potentially important prerequisite for vesicle fusion to the plasma membrane and may be required also for other non-secretory vesicles, depicting a general mechanism for vesicle fusion.

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‘Porosome’ discovered nearly 20 years ago provides molecular insights into the kiss‐and‐run mechanism of cell secretion

Cited by 18 publications

References 53 publications

Development of pancreatic acini in embryos of the grass snake Natrix natrix (Lepidosauria, Serpentes)

Development of pancreatic acini in embryos of the grass snake Natrix natrix (Lepidosauria, Serpentes)

Possible Mechanism of Porosome-Mediated Exosome Release

Gi Protein Modulation of the Potassium Channel TASK-2 Mediates Vesicle Osmotic Swelling to Facilitate the Fusion of Aquaporin-2 Water Channel Containing Vesicles

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