Poro-viscoelastic behaviour of the zona pellucida: Impact of three-dimensional modelling on material characterisation

Karimian, Kian; Seydewitz, Robert; Töpfer, Dagmar; Böl, Markus

doi:10.1016/j.jmbbm.2022.105211

Cited by 6 publications

(2 citation statements)

References 82 publications

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“…Another explanation could be the poroelasticity of the zona pellucida [12]. In one study, the timescale of poroelasticity was cited as roughly three orders of magnitude faster than that of the relaxation in the zona pellucida [65]. While our shallow indentations likely did not induce great volume changes, this mechanism could have influenced our observed force response.…”

Section: Sharp Indentation: Local Response Reveals Softening Of the Z...mentioning

confidence: 83%

Profiling oocytes with neural networks from images and mechanical data

Lamont

Fropier

Abadie

et al. 2023

Journal of the Mechanical Behavior of Biomedical Materials

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Section: Sharp Indentation: Local Response Reveals Softening Of the Z...mentioning

confidence: 83%

Profiling oocytes with neural networks from images and mechanical data

Lamont

Fropier

Abadie

et al. 2023

Journal of the Mechanical Behavior of Biomedical Materials

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“…The fact that oocytes are virus-positive is of importance. The oocytes are usually surrounded by the zona pellucida (ZP), an extracellular matrix of glycoproteins building a three-dimensional structure which should prevent virus infection [22]. It is widely recognized that a virus infection is only possible after damaging the ZP.…”

Section: Discussionmentioning

confidence: 99%

Detection of porcine cytomegalovirus, a roseolovirus, in pig ovaries and follicular fluid: implications for somatic cells nuclear transfer, cloning and xenotransplantation

Hansen

Fischer

Krabben

et al. 2023

Virol J

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Background Porcine cytomegalovirus (PCMV) is a porcine roseolovirus (PCMV/PRV) which is widely distributed in pigs. Transmission of PCMV/PRV in preclinical xenotransplantations was shown to significantly reduce the survival time of the pig transplants in non-human primates. PCMV/PRV was also transmitted in the first transplantation of a pig heart into a human patient. To analyze how PCMV/PRV could be introduced into pig breeds, especially considering cloned transgenic pigs, and subsequently spread in breeding facilities, we screened ovaries and derived materials which are used to perform somatic cell nuclear transfer (SCNT). Methods DNA was isolated from ovarian tissues, follicular fluids, oocytes with cumulus cells, denuded oocytes and parthenotes. A real-time PCR with PCMV/PRV-specific primers and a probe was performed to detect PCMV/PRV. Furthermore, a Western blot assay using a recombinant fragment of the gB protein of PCMV/PRV was performed to screen for virus-specific antibodies in the follicular fluids. Results PCMV/PRV was found by real-time PCR in ovarian tissues, in the follicular fluid and in oocytes. In parthenotes the virus could not be detected, most-likely due to the low amount of DNA used. By Western blot assay specific antibodies against PCMV/PRV were found in 19 of 20 analyzed follicular fluids. Conclusion PCMV/PRV was found in ovarian tissues, in the follicular fluids and also in denuded oocytes, indicating that the virus is present in the animals of which the oocytes were taken from. Despite several washing steps of the denuded oocytes, which are subsequently used for microinjection or SCNT, the virus could still be detected. Therefore, the virus could infect oocytes during genetic modifications or stay attached to the surface of the oocytes, potentially infecting SCNT recipient animals.

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The full model of micropipette aspiration of cells: A mesoscopic simulation

Zhang

Cheng

et al. 2023

Acta Biomaterialia

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Poro-viscoelastic behaviour of the zona pellucida: Impact of three-dimensional modelling on material characterisation

Cited by 6 publications

References 82 publications

Profiling oocytes with neural networks from images and mechanical data

Profiling oocytes with neural networks from images and mechanical data

Detection of porcine cytomegalovirus, a roseolovirus, in pig ovaries and follicular fluid: implications for somatic cells nuclear transfer, cloning and xenotransplantation

The full model of micropipette aspiration of cells: A mesoscopic simulation

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