Pore Size Variability of DNA Nanomeshes by Polymerase Chain Reaction

Investigation of robust and efficient pathways to build DNA-organic molecule hybrid structures is fundamentally important to realize controlled placement of single molecules for potential applications, such as single-molecule electronic devices. Herein, we report a systematic investigation of synthetic processes for preparing organic molecule-DNA building blocks and their subsequent elongation to generate precise micrometer-sized structures. Specifically, optimal cross-coupling routes were identified to enable chemical linkages between three different organic molecules, namely, polyethylene glycol (PEG), poly(p-phenylene ethynylene) (PPE), and benzenetricarboxylate, with single-stranded (ss) DNA. The resulting DNA-organic molecule hybrid building blocks were purified and characterized by both denaturing gel electrophoresis and electrospray ionization mass spectrometry (ESI-MS). The building blocks were subsequently elongated through both the DNA hybridization and ligation processes to prepare micrometer-sized double-stranded (ds) DNA-organic molecule hybrid structures. The described synthetic procedures should facilitate future syntheses of various hybrid DNA-based organic molecular structures.

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The use of enzymes for construction of DNA-based objects and assemblies

Keller

Marx

2011

Chem. Soc. Rev.

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DNA has found wide applications in DNA-based nanotechnology due to its simplicity and predictability of its secondary structure. Selecting DNA for the nanoconstruction of objects and assemblies bears the inherent potential for manipulations and control by DNA modifying enzymes. In this tutorial review, we present an overview of the enzyme-catalysed construction of DNA-based objects and assemblies. It is illustrated how a diversity of enzyme-based biochemical reactions are transferred in nanotechnological applications.

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Pore Size Variability of DNA Nanomeshes by Polymerase Chain Reaction

Cited by 5 publications

References 28 publications

Surface‐Initiated, Reversible Polymerization from Surface‐Tethered Oligonucleotides by Enzymatic Processes

Surface‐Initiated, Reversible Polymerization from Surface‐Tethered Oligonucleotides by Enzymatic Processes

Syntheses of Organic Molecule−DNA Hybrid Structures

The use of enzymes for construction of DNA-based objects and assemblies

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