Pore Size Tunable Trypsin@ZIF-90 and Hydrogel Integrated Lateral Flow Point-of-Care Platform for ATP Detection

Liu, Wei; Deng, Yuan; Yuan, Yuni; Ouyang, Stephen L.; Liu, Qi; Chen, Xiao‐Qing

doi:10.1021/acsami.3c02888

Cited by 8 publications

(1 citation statement)

References 41 publications

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“…The results suggested that Au/Pt NPs were simply physically trapped within the ZIF-90 framework without participating in the crystallization process of ZIF-90. Building upon our previous work, 35 these results further substantiate the discernible dissimilarity in the mechanisms employed for bioenzymes and nanoenzymes encapsulation utilizing ZIF-90. The nitrogen adsorption isotherms and the pore size distribution data obtained through FLDFT calculations (Figure 2B,C) further support this observation, as they exhibit nearly overlapping characteristics within the same range.…”

Section: ■ Results and Discussionsupporting

confidence: 79%

Au/Pt@ZIF-90 Nanoenzyme Capsule-Based “Explosive” Signal Amplifier for “All-in-Tube” POCT

Liu,

Yao,

Liu

et al. 2024

Anal. Chem.

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The sensitive, convenient, and visual detection of low-concentration disease markers in biological samples has always been a priority in disease diagnosis. However, existing research has been problematic due to complex operation and unsatisfactory sensitivity. Consequently, an "explosive" signal amplification platform based on Au/Pt@ZIF-90 was developed for sensitive visual detection of disease markers. In this study, a controllable and explosively released Au/Pt nanoparticles (NPs) "nanoenzyme capsule" was prepared by encapsulating Au/Pt NPs with excellent peroxidase activity in ZIF-90. This was achieved by adjusting the particle size of ZIF-90 and the encapsulation amount of Au/Pt NPs. Using the prepared capsules as the signal output module and aptamer as the target recognition module, an "All-in-Tube" portable point-of-care (POC) platform was constructed by integrating the Au/Pt@ZIF-90/filter paper and TMB/strips into an Eppendorf (EP) tube. By utilizing specific competitive binding of targets to aptamers, the platform enabled the sensitive and convenient measurement of small molecular disease markers. Taking adenosine as the proof of concept, the portable detection achieved excellent sensitivity. Moreover, the platform can achieve universal detection of various targets by varying the aptamer sequence. This signal amplification strategy provides a design pattern for the detection of lowconcentration targets in biological samples and holds significant potential in the fields of disease diagnosis and environmental monitoring.

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Section: ■ Results and Discussionsupporting

confidence: 79%

Au/Pt@ZIF-90 Nanoenzyme Capsule-Based “Explosive” Signal Amplifier for “All-in-Tube” POCT

Liu,

Yao,

Liu

et al. 2024

Anal. Chem.

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Tailored Micromagnet Sorting Gate for Simultaneous Multiple Cell Screening in Portable Magnetophoretic Cell‐On‐Chip Platforms

Yoon,

Kang,

Kim

et al. 2024

Adv Funct Materials

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Conventional magnetophoresis techniques for manipulating biocarriers and cells predominantly rely on large‐scale electromagnetic systems, which is a major obstacle to the development of portable and miniaturized cell‐on‐chip platforms. Herein, a novel magnetic engineering approach by tailoring a nanoscale notch on a disk micromagnet using two‐step optical and thermal lithography is developed. Versatile manipulations are demonstrated, such as separation and trapping, of carriers and cells by mediating changes in the magnetic domain structure and discontinuous movement of magnetic energy wells around the circumferential edge of the micromagnet caused by a locally fabricated nano‐notch in a low magnetic field system. The motion of the magnetic energy well is regulated by the configuration of the nanoscale notch and the strength and frequency of the magnetic field, accompanying the jump motion of the carriers. The proposed concepts demonstrate that multiple carriers and cells can be manipulated and sorted using optimized nanoscale multi‐notch gates for a portable magnetophoretic system. This highlights the potential for developing cost‐effective point‐of‐care testing and lab‐on‐chip systems for various single‐cell‐level diagnoses and analyses.

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Recent Advances in Biomacromolecule‐Reinforced 2D Material (2DM) Hydrogels: From Interactions, Synthesis, and Functionalization to Biomedical Applications

Gu,

Cui,

et al. 2024

Adv Funct Materials

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Regenerative biomedicine has emerged as a forefront area in medical research, heralding a new era of therapeutic strategies. This review delineates the integration of 2D materials (2DMs) within the area of biomedical engineering, leveraging their superior physicochemical attributes for enhance biomedical outcomes. The synergistic interaction between biomacromolecules and 2DMs is explored, demonstrating their potential to mitigate the limitations inherent to each while simultaneously augmenting their beneficial properties. In particular, incorporating 2DMs into hydrogels highlights their capability to enhance the mechanical strength of hydrogels, providing a biomimetic scaffold for tissue engineering regeneration and cancer diagnosis and therapy. An overview of the synthetic methodologies are provided for 2DMs, elucidating their interaction dynamics with biomacromolecules. The review primarily concentrates on the applications of biomacromolecule‐reinforced 2DM hydrogels across various biomedical fields, including bone tissue engineering, wound healing, neural tissue engineering, cardiac tissue engineering, as well as in the delivery of drugs and genes, cancer therapy, and biosensing technologies. Finally, the review discusses the existing challenges and future outlook for developing and using biomacromolecule‐reinforced 2DM hydrogels, underlining their transformative potential in regenerative medicine.

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Pore Size Tunable Trypsin@ZIF-90 and Hydrogel Integrated Lateral Flow Point-of-Care Platform for ATP Detection

Cited by 8 publications

References 41 publications

Au/Pt@ZIF-90 Nanoenzyme Capsule-Based “Explosive” Signal Amplifier for “All-in-Tube” POCT

Au/Pt@ZIF-90 Nanoenzyme Capsule-Based “Explosive” Signal Amplifier for “All-in-Tube” POCT

Tailored Micromagnet Sorting Gate for Simultaneous Multiple Cell Screening in Portable Magnetophoretic Cell‐On‐Chip Platforms

Recent Advances in Biomacromolecule‐Reinforced 2D Material (2DM) Hydrogels: From Interactions, Synthesis, and Functionalization to Biomedical Applications

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