Pore size modulates in vitro osteogenesis of bone marrow mesenchymal stem cells in fibronectin/gelatin coated silk fibroin scaffolds

Ai, Chengchong; Liu, Ling; Goh, James Cho‐Hong

doi:10.1016/j.msec.2021.112088

Cited by 25 publications

(13 citation statements)

References 49 publications

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“…It can be seen that the OD value of each sample increased significantly with the extension of culture time, indicating that cells could normally grow and proliferate on different membrane surfaces. In addition, the OD values of freeze-dried composite membrane were significantly higher than those of the membrane dried in air or in oven, which further indicated that the porous structure of freeze-dried composite membrane was more conducive for cell proliferation and had good biocompatibility (Ai, et al, 2021). Comparing to the n-HA/BF membranes with 10%, 20% and 30%, the OD value enhanced significantly with the increase of n-HA content and the extension of culture time.…”

Section: Mtt Test Of Cell Culturementioning

confidence: 86%

Effect of Different Preparation Conditions On The Properties of Bamboo Fiber-Based Bioactive Composite Membrane

Liuyun

Jiang

et al. 2022

Preprint

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A novel nano-hydroxyapatite/bamboo fiber (n-HA/BF) bioactive composite membrane was obtained by a simple casting technique. The membrane forming mechanism and the effects of different forming membrane methods, drying methods and n-HA amounts on the properties of n-HA/BF membrane were investigated by Fourier Transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (SEM), contact angle, electromechanical universal tester, in vitro soaking in simulated body fluid (SBF) and in vitro cell cultureexperiment. The results demonstrated that the n-HA dispersity in BF matix was not affected by the prepartion condition, however, the morphologies of membrane was determined by the different preparation conditions owing to different hydrogen bond shrinkage. Moreover, the hydrophilicity of the composite membrane was improved under the condition of the membrane formation in oven, freeze drying and high addition content of n-HA, and the mechanical properties of composite membrane depended on n-HA content. In vitro soaking behavior indicated that the degradability and bone-like apatite deposition could be controled by differentpreparation conditions. And the cell proliferation experiment showed that the n-HA/BF composite membranes obtained under different preparation conditions were all non-toxic. The above results indicated that the n-HA/BF composite membrane could be obtained by a simple casting technique, and the properties could be controlled by adopting different preparation conditions, which would have a great promising as guide bone tissue regeneration (GBR) membrane, and the study would provide a new application for BF in biomedical field.

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Section: Mtt Test Of Cell Culturementioning

confidence: 86%

Effect of Different Preparation Conditions On The Properties of Bamboo Fiber-Based Bioactive Composite Membrane

Liuyun

Jiang

et al. 2022

Preprint

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“…The change of grain size and The grain orientation variations of the characteristic positions after bending are regulated as follows: The (001) grain orientation of the lower end plate is more chaotic than that of the upper one (Figure 14). The original orientation is weak due to the low Hall-Pitch coefficient for the aluminum matrix [4]. The texture of the bent upper end plate on width direction is stronger than that of the lower one.…”

Section: Microstructure Analysismentioning

confidence: 99%

“…SLM manufactured parts occupy widespread application because of light density [1][2][3] and complex modeling, which present challenges in mechanical machining [4]. For example, the SLM parts are manufactured into bracket connectors on the Airbus A350 XWB [5] and could also be designed as cooling channels with optimized mechanical and heat dissipation performance [6], which are employed in the combustion chambers or turbines of aircraft engine.…”

Section: Introductionmentioning

confidence: 99%

Force and Microstructure Variation of SLM Prepared AlMgSc Samples during Three-Point Bending

Nie

Zhang

et al. 2022

Materials

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Lightweight parts manufactured by metal selective laser melting (SLM) are widely applied in machinery industries because of their high specific strength, good energy absorption effect, and complex shape that are difficult to form by mechanical machining. These samples often serve in three-dimensional stress states. However, previous publications mainly focused on the unidirectional tensile/compressive properties of the samples. In this paper, AlMgSc samples with different geometric parameters were prepared by the SLM process, and the variation of force and microstructure during three-point bending were systematically investigated. The results demonstrate that the deformation resistance of these samples has good continuity without mutation in bending, even for brittle materials; the bending force-displacement curves exhibit representative variation stages during the entire bending process; the equivalent bending strength deduced from free bending formula is not applicable when compactability is less than 67%. The variations of grain orientation and size of the three representative bending layers also show regularity.

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“…[142] SF is an attractive biopolymer for bone tissue engineering because of its biocompatibility, good mechanical properties, and biodegradability. [143,144] However, the mechanical properties of SF still need to be improved to mimic native bone tissue, and since SF is proteinaceous, it is likely to degrade too rapidly in vivo, at a rate not matching the rate of bone regeneration. [143] Moreover, SF lacks sufficient bioactivity to enhance the adhesion, proliferation, and differentiation of osteoblast cells.…”

Section: Bone Biomaterialsmentioning

confidence: 99%

Immune Response to Silk Sericin–Fibroin Composites: Potential Immunogenic Elements and Alternatives for Immunomodulation

Boni

Bakadia

Osi

et al. 2021

Macromolecular Bioscience

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The unique properties of silk proteins (SPs), particularly silk sericin (SS) and silk fibroin (SF), have attracted attention in the design of scaffolds for tissue engineering over the past decades. Since SF has good mechanical properties, while SS displays bioactivity, scaffolds combining both proteins should exhibit complementary properties enhancing the potential of these materials. Unfortunately, SS-SF composites can generate chronic immune responses and their immunogenic element is not completely clear. The potential of SS-SF composites in tissue engineering, elements which may contribute to their immunogenicity, and alternatives for their preparation and design, to modulate the immune response and take advantage of their useful properties, are discussed in this review. It is known that SS can enhance 𝜷-sheet formation in SF, which may act as hydrophobic regions with a strong affinity for adsorption proteins inducing the chronic recruitment of inflammatory cells. Therefore, tailoring the exposure of hydrophobic regions at the scaffold surface should represent a viable strategy to modulate the immune response. This can be achieved by coating SS-SF composites with SS or other hydrophilic polymers, to take advantage of their antibiofouling properties. Research is still needed to realize the full potential of these composites for tissue engineering.

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Pore size modulates in vitro osteogenesis of bone marrow mesenchymal stem cells in fibronectin/gelatin coated silk fibroin scaffolds

Cited by 25 publications

References 49 publications

Effect of Different Preparation Conditions On The Properties of Bamboo Fiber-Based Bioactive Composite Membrane

Effect of Different Preparation Conditions On The Properties of Bamboo Fiber-Based Bioactive Composite Membrane

Force and Microstructure Variation of SLM Prepared AlMgSc Samples during Three-Point Bending

Immune Response to Silk Sericin–Fibroin Composites: Potential Immunogenic Elements and Alternatives for Immunomodulation

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