Pore size effect of collagen scaffolds on cartilage regeneration

Zhang, Qin; Lu, Hongxu; Naoki, Katsuhiko; Chen, Guoping

doi:10.1016/j.actbio.2013.12.042

Cited by 275 publications

(191 citation statements)

References 44 publications

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“…However, smaller pores induced a more 3D adhesion configuration for cells in smaller pores, where cells have a limited surface on which they can proliferate in monolayer and are thus stimulated to aggregate in 3D, as shown in Figure 2D. Cell aggregation is an important cue for phenotypic expression of chondrocyte markers in most published reports in the literature (3,(14)(15)(16)27). In addition, hypoxia is recognized as a stimulus leading to an increased expression of the cartilage phenotype in chondrocytes.…”

Section: Resultsmentioning

confidence: 99%

“…An appropriate physicochemical environment that accounts for a physiological intracellular stress state deriving from the cell-biomaterial interaction may favor the recovery of a phenotypic cell expression (1,2). The maintenance of such a physiological adhesive configuration, which is primarily related to the microarchitecture of the scaffold, (8,12,13,(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27). However, some results have indicated that chondrocyte phenotype and biosynthetic activity improved in collagen matrices containing smaller pores (28,29).…”

Section: Introductionmentioning

confidence: 99%

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The Effect of Scaffold Pore Size in Cartilage Tissue Engineering

Nava

Draghi

Giordano

et al. 2016

Journal of Applied Biomaterials & Functional Materials

104

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Introduction: The effect of scaffold pore size and interconnectivity is undoubtedly a crucial factor for most tissue engineering applications. The aim of this study was to examine the effect of pore size and porosity on cartilage construct development in different scaffolds seeded with articular chondrocytes. Methods: We fabricated poly-L-lactide-co-trimethylene carbonate scaffolds with different pore sizes, using a solvent-casting/particulate-leaching technique. We seeded primary bovine articular chondrocytes on these scaffolds, cultured the constructs for 2 weeks and examined cell proliferation, viability and cell-specific production of cartilaginous extracellular matrix proteins, including GAG and collagen. Results: Cell density significantly increased up to 50% with scaffold pore size and porosity, likely facilitated by cell spreading on the internal surface of bigger pores, and by increased mass transport of gases and nutrients to cells, and catabolite removal from cells, allowed by lower diffusion barriers in scaffolds with a higher porosity. However, both the cell metabolic activity and the synthesis of cartilaginous matrix proteins significantly decreased by up to 40% with pore size. We propose that the association of smaller pore diameters, causing 3-dimensional cell aggregation, to a lower oxygenation caused by a lower porosity, could have been the condition that increased the cell-specific synthesis of cartilaginous matrix proteins in the scaffold with the smallest pores and the lowest porosity among those tested. Conclusions: In the initial steps of in vitro cartilage engineering, the combination of small scaffold pores and low porosity is an effective strategy with regard to the promotion of chondrogenesis

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Effect of Scaffold Pore Size in Cartilage Tissue Engineering

Nava

Draghi

Giordano

et al. 2016

Journal of Applied Biomaterials & Functional Materials

104

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“…Several studies have reported that pore sizes were important factors that influenced cell adhesion and differentiation on the porous scaffolds 4,15,33,34) . In our study, the ACG sponge fabricated with lyophilization had a mean pore size of about 90-115 μm.…”

Section: Discussionmentioning

confidence: 99%

“…Lyophilization (also called freeze-drying) has been proven as an effective method to fabricate porous scaffolds 4,[12][13][14] . Porous collagen scaffolds were successfully fabricated using solvents such as ethanol and acetic acid, and glutaraldehyde for chemical crosslink reactions 15) . Gelatin scaffolds with controlled pore structures were also fabricated using lyophilization 14) .…”

Section: Introductionmentioning

confidence: 99%

Influence of lyophilization factors and gelatin concentration on pore structures of atelocollagen/gelatin sponge biomaterial

et al. 2017

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This study aimed to investigate influences of lyophilization factors and gelatin concentration on pore structures of ACG sponge. ACG sponges of different freezing temperatures (−30, −80 and −196 o C), freezing times (1, 2 and 24 h), gelatin concentrations (0.6%AC+0.15%G, 0.6%AC+0.6%G and 0.6%AC+2.4%G), and with 500 μM fluvastatin were fabricated. Pore structures including porosity and pore size were analyzed by scanning electron microscopy and ImageJ. The cytotoxic effects of ACG sponges were evaluated in vitro. Freezing temperature did not affect porosity while high freezing temperature (−30 o C) increased pore size. The high gelatin concentration group (0.6%AC+2.4%G) had decreased porosity and pore size. Freezing time and 500 μM fluvastatin did not affect pore structures. The cytotoxicity and cell proliferation assays revealed that ACG sponges had no cytotoxic effects on human mesenchymal stromal cell growth and proliferation. These results indicate that ACG sponge may be a good biomaterial scaffold for bone regeneration.

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“…For most of the porous scaffolds, pore size and porosity have great effect on cell attachment, growth, proliferation and differentiate [11]. Zhao et al [12], Zhang et al [13], Oh et al [14], Huri et al [15] prove cell behavior can be affected by the pore size, but there are still no convincing conclusions illustrating the relationship between pore size and cell behavior. Swieszkowski et al [16] developed two kinds of hybrid scaffolds via fused deposition modeling then seeded bone marrow mesenchymal cells (MSC) into the scaffolds.…”

Section: Introductionmentioning

confidence: 99%

A Novel Biphasic Scaffold with Gradient Pore Structures for Osteochondral Tissue Engineering

Pan¹,

Yin²,

Yang³

et al. 2017

Proceedings of the 2016 International Conference on Biological Engineering and Pharmacy (BEP 2016)

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Abstract-This study was aimed at using biomimetic strategies to construct a biphasic scaffold. Biphasic scaffolds were constructed using the 3-dimentional printing technology with two phases: the bony phase and the chondral phase. The bony phase was using the composition of poly (lactide-co-glycolide) (PLGA) and Polyethylene glycol PEG; the chondral phase was using hydrogel consisting of GelMA and hyaluronic acid. Also we designed a gradient pore size in the bony phase to better mimic the natural bone tissue. In vitro tests have shown the stratified scaffolds with gradient designs were effective for the multi-tissue formation.

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Pore size effect of collagen scaffolds on cartilage regeneration

Cited by 275 publications

References 44 publications

The Effect of Scaffold Pore Size in Cartilage Tissue Engineering

The Effect of Scaffold Pore Size in Cartilage Tissue Engineering

Influence of lyophilization factors and gelatin concentration on pore structures of atelocollagen/gelatin sponge biomaterial

A Novel Biphasic Scaffold with Gradient Pore Structures for Osteochondral Tissue Engineering

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