Pore-expanded mesoporous silica nanoparticles with alkanes/ethanol as pore expanding agent

Kao, Kun‐Che; Mou, Chung‐Yuan

doi:10.1016/j.micromeso.2012.09.030

Cited by 85 publications

(69 citation statements)

References 36 publications

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“…[52] Silica NPs with pore sizes ranging from 5 nm up to 40 nm have been utilized to load proteins of various molecular weights from cytochrome C (12 kDa) to immunoglobulin G (150 kDa). [53][54][55][56][57][58][59] Iron oxide@mesoporous silica NPs with 5-9 nm [60] and 10-15 nm [61] pores were also developed and loaded with biomolecules of sizes below 4 nm. [52] The large pore sizes available with the elaboration of novel inorganic…”

Section: Accepted M Manuscriptmentioning

confidence: 99%

Biodegradable Magnetic Silica@Iron Oxide Nanovectors with Ultra-Large Mesopores for High Protein Loading, Magnetothermal Release, and Delivery

Omar

Croissant

Alamoudi

et al. 2017

Journal of Controlled Release

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Abstract:The delivery of large cargos of diameter above 15 nm for biomedical applications has proved challenging since it requires biocompatible, stably-loaded, and biodegradable nanomaterials. In this study, we describe the design of biodegradable silica-iron oxide hybrid nanovectors with large mesopores for large protein delivery in cancer cells. The mesopores of the nanomaterials spanned from 20 to 60 nm in diameter and post-functionalization allowed the electrostatic immobilization of large proteins (e.g. mTFP-Ferritin, ~534 kDa). Half of the content of the nanovectors was based with iron oxide nanophases which allowed the rapid biodegradation of the carrier in fetal bovine serum and a magnetic responsiveness. The nanovectors released large protein cargos in aqueous solution under acidic pH or magnetic stimuli. The delivery of large proteins was then autonomously achieved in cancer cells via the silica-iron oxide nanovectors, which is thus a promising for biomedical applications.

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Section: Accepted M Manuscriptmentioning

confidence: 99%

Biodegradable Magnetic Silica@Iron Oxide Nanovectors with Ultra-Large Mesopores for High Protein Loading, Magnetothermal Release, and Delivery

Omar

Croissant

Alamoudi

et al. 2017

Journal of Controlled Release

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“…The addition of TEOS and ethanol to the solution containing micelles of surfactants with stirring initiates the formation of silica spheres whose mesopores are formed toward their centers [9]. Hexadecyltrimethylammonium bromide (CTAB) is a typical surfactant used in the preparation of mesoporous silica spheres [10,11]. The replacement of ethanol by acetone is also effective in producing mesopores in silica spheres with high reproducibility [12,13].…”

Section: Introductionmentioning

confidence: 99%

Preparation of dandelion-type silica spheres and their application as catalyst supports

et al. 2014

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Dandelion-type silica spheres with a dendrimer-like porous structure were prepared by adding pore modifiers into aqueous synthetic mixtures of tetraethylorthosilicate (TEOS), hexadecyltrimethylammonium bromide (CTAB), ammonium hydroxide, and acetone. The formation of silica spheres and their porous characteristics were investigated using various techniques, including electron microscopy, nitrogen adsorption, and thermogravimetric analysis. Benzyl acetate (BENA) was very effective in the formation of a dendrimer-like porous structure. However, the composition of TEOS, CTAB, acetone, and BENA strongly influenced the size and shape of the silica spheres and their porous structure. The synthetic mixture of 1 TEOS: 0.22 CTAB: 1.9 BENA: 0.32 NH 4 OH: 36 acetone: 236 H 2 O produced dandelion-type silica spheres with diameters of *300 nm. The phosphazenium hydroxide (PzOH) catalyst supported on the dandelion-type silica spheres prepared by adding BENA showed high catalytic performance in the transesterification of soybean oil with methanol due to its high feasibility for rapid access of large triglyceride molecules into the basic PzOH moieties incorporated in the pores.

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“…This was most notably demonstrated via the immobilization of lysozyme into pore expanded MSN (5.6 nm) and conventional MSN (2.5 nm). After comparing the results, it was observed that pore expanded MSN materials had larger adsorption capacity (420 mg lysozyme g -1 MSN) than the conventional MSN materials (340 mg lysozyme g-1 MSN) [41]. These results indicated that the pore expanded MSN materials would make superior nanovehicles for proteins.…”

Section: Pore Expanding Agentsmentioning

confidence: 93%

“…An alternate strategy for controlling the particle size, pore size, and pore structure is to tune the concentration of aqueous ammonia (NH 4 OH) in ethanol. This method generated multiple combinations of pore-expanded MSN [41]. Gu et al were able to synthesize large pore MSN (4.6 nm) materials with particle diameter less than 150 nm using a pore size mediator DMHA (N,N-dimethylhexadecylamine), the cationic surfactant CTAB (cetyltrimethylammonium bromide) acting as a templating agent, and the triblock copolymer Pluronic F127 as a particle growth inhibitor [42].…”

Section: Pore Expanding Agentsmentioning

confidence: 99%

Controlled release and intracellular protein delivery from mesoporous silica nanoparticles

Deodhar

Adams

Trewyn

2016

Biotechnology Journal

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Protein therapeutics are promising candidates for disease treatment due to their high specificity and minimal adverse side effects; however, targeted protein delivery to specific sites has proven challenging. Mesoporous silica nanoparticles (MSN) have demonstrated to be ideal candidates for this application, given their high loading capacity, biocompatibility, and ability to protect host molecules from degradation. These materials exhibit tunable pore sizes, shapes and volumes, and surfaces which can be easily functionalized. This serves to control the movement of molecules in and out of the pores, thus entrapping guest molecules until a specific stimulus triggers release. In this review, we will cover the benefits of using MSN as protein therapeutic carriers, demonstrating that there is great diversity in the ways MSN can be used to service proteins. Methods for controlling the physical dimensions of pores via synthetic conditions, applications of therapeutic protein loaded MSN materials in cancer therapies, delivering protein loaded MSN materials to plant cells using biolistic methods, and common stimuli‐responsive functionalities will be discussed. New and exciting strategies for controlled release and manipulation of proteins are also covered in this review. While research in this area has advanced substantially, we conclude this review with future challenges to be tackled by the scientific community.

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Pore-expanded mesoporous silica nanoparticles with alkanes/ethanol as pore expanding agent

Cited by 85 publications

References 36 publications

Biodegradable Magnetic Silica@Iron Oxide Nanovectors with Ultra-Large Mesopores for High Protein Loading, Magnetothermal Release, and Delivery

Biodegradable Magnetic Silica@Iron Oxide Nanovectors with Ultra-Large Mesopores for High Protein Loading, Magnetothermal Release, and Delivery

Preparation of dandelion-type silica spheres and their application as catalyst supports

Controlled release and intracellular protein delivery from mesoporous silica nanoparticles

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