Pore dynamics in lipid membranes

Gözen, İrep; Dommersnes, Paul

doi:10.1140/epjst/e2014-02228-5

Cited by 34 publications

(31 citation statements)

References 86 publications

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“…It is important to note that the non-trivial ruptures do not occur in extreme conditions but at moderate tensile stress, with similar values to those observed in living cells 28,29 . Such fractures are therefore anticipated to occur in vivo, although the findings are relatively new and currently very little is known regarding the formation of fractures in living organisms.…”

Section: Resultssupporting

confidence: 71%

A cellular automaton for modeling non-trivial biomembrane ruptures

Gupta

Reint

Gözen

et al. 2018

Preprint

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A novel cellular automaton (CA) for simulating biological membrane rupture is proposed. Constructed via simple rules governing deformation, tension, and fracture, the CA incorporates ideas from standard percolation models and bond-based fracture methods. The model is demonstrated by comparing simulations with experimental results of a double bilayer lipid membrane expanding on a solid substrate. Results indicate that the CA can capture non-trivial rupture morphologies such as floral patterns and the saltatory dynamics of fractal avalanches observed in experiments. Moreover, the CA provides insight into the poorly understood role of inter-layer adhesion, supporting the hypothesis that the density of adhesion sites governs rupture morphology.

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Section: Resultssupporting

confidence: 71%

A cellular automaton for modeling non-trivial biomembrane ruptures

Gupta

Reint

Gözen

et al. 2018

Preprint

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“…The existence of many small rigid regions within a liquid membrane may lead to percolation, sub-diffusive behavior and shear response[45]. The dynamics of spreading membranes on solid supports is mainly dominated by friction rather than viscosity[23]. Friction in conjunction with local Ca 2+ -induced rigidity can also hinder membrane flow and promote shear response in the distal bilayer.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, we also neglect the effect of the fluid surrounding the membrane. In fact, even in case of fully fluid-surrounded membranes, for example in vesicles, dissipation due to the flow of the membrane will dominate the pore dynamics compared to the dissipation caused by the water flowing through an opening pore, since the membrane is more viscous compared to water[23]. …”

Section: Methodsmentioning

confidence: 99%

Peridynamic Modeling of Ruptures in Biomembranes

et al. 2016

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We simulate the formation of spontaneous ruptures in supported phospholipid double bilayer membranes, using peridynamic modeling. Experiments performed on spreading double bilayers typically show two distinct kinds of ruptures, floral and fractal, which form spontaneously in the distal (upper) bilayer at late stages of double bilayer formation on high energy substrates. It is, however, currently unresolved which factors govern the occurrence of either rupture type. Variations in the distance between the two bilayers, and the occurrence of interconnections (“pinning sites”) are suspected of contributing to the process. Our new simulations indicate that the pinned regions which form, presumably due to Ca2+ ions serving as bridging agent between the distal and the proximal bilayer, act as nucleation sites for the ruptures. Moreover, assuming that the pinning sites cause a non-zero shear modulus, our simulations also show that they change the rupture mode from floral to fractal. At zero shear modulus the pores appear to be circular, subsequently evolving into floral pores. With increasing shear modulus the pore edges start to branch, favoring fractal morphologies. We conclude that the pinning sites may indirectly determine the rupture morphology by contributing to shear stress in the distal membrane.

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“…When a large positive tension is applied on a biomembrane, pore formation occurs in the membrane, causing rupture of cells or liposomes. To reveal the kinetics and the mechanism of tension-induced pore formation, giant unilamellar vesicles (GUVs) have been used [1][2][3][4][5]. By the use of a viscous solvent, Brochard-Wyart et al visualized directly at video rate the fast dynamics of pore openings and closings in a GUV which was stretched as a result of intense optical illumination [1].…”

Section: Introductionmentioning

confidence: 99%

Electrostatic interaction effects on tension-induced pore formation in lipid membranes

et al. 2015

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We investigated the effects of electrostatic interactions on the rate constant (k(p)) for tension-induced pore formation in lipid membranes of giant unilamellar vesicles under constant applied tension. A decrease in salt concentration in solution as well as an increase in surface charge density of the membranes increased k(p). These data indicate that k(p) increases as the extent of electrostatic interaction increases. We developed a theory on the effect of the electrostatic interactions on the free energy profile of the membrane containing a prepore and also on the values of k(p); this theory explains the experimental results and fits the experimental data reasonably well in the presence of weak electrostatic interactions. Based on these results, we conclude that a decrease in the free energy barrier of the prepore state due to electrostatic interactions is the main factor causing an increase in k(p).

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Pore dynamics in lipid membranes

Cited by 34 publications

References 86 publications

A cellular automaton for modeling non-trivial biomembrane ruptures

A cellular automaton for modeling non-trivial biomembrane ruptures

Peridynamic Modeling of Ruptures in Biomembranes

Electrostatic interaction effects on tension-induced pore formation in lipid membranes

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