Porcine uterus contains a population of mesenchymal stem cells

Miernik, Katarzyna; Karasiński, J

doi:10.1530/rep-11-0202

Cited by 45 publications

(56 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our cytometric results suggest that stem cells constitute a small population of the cells in the bovine uterus. This suggestion is consistent with studies on women, mice, and pigs, in which different stem cell identification approaches showed that in the uterine tissue of these species the pluripotent/multipotent cells populations were minor (Chan et al 2004, Teixeira et al 2008, Miernik & Karasinski 2012. Stem cells also persist in small amounts throughout life in other regenerative adult tissues such as epithelia, bone marrow, and dental pulp (Pittenger et al 1999, Gronthos et al 2000, Slack 2000.…”

Section: Discussionsupporting

confidence: 89%

Identification of pluripotent cells in bovine uterus: in situ and in vitro studies

Łupicka

Bodek

Shpigel³

et al. 2015

Reproduction

View full text Add to dashboard Cite

The aim of this study was to identify uterine pluripotent cells both in bovine uterine tissues as well in epithelial, stromal, and myometrial uterine cell populations. Moreover, the relationship of pluripotent markers expression with age and the uterine horn side was considered. Uterine tissue was collected from ipsilateral and contralateral horns (days 8-10 of the estrous cycle). Immunohistostaining for C-KIT, OCT3/4, NANOG, and SOX2 in uterine tissue was determined. mRNA expression of C-KIT, OCT3/4, NANOG and SOX2 was evaluated in uterine tissue relative to the age of the cow and uterine horn side. Gene and protein expression of these markers in the uterine luminal epithelial, stromal, and myometrial cells was evaluated by real-time PCR and western blotting respectively. The expression of pluripotent cell markers OCT3/4, NANOG, and SOX2 was identified by flow cytometry assay in epithelial, stromal, and myometrial cells. Multilineage differentiation of the bovine uterine cells was performed. mRNA expression of OCT3/4, NANOG, and SOX2 in uterine tissue was higher in the ipsilateral horn than in the contralateral horn. Flow cytometry assay revealed positive fluorescence for OCT3/4, NANOG, and SOX2 in all uterine cell types. Results showed the age-dependent expression of pluripotent markers in uterine tissue. Beside, the different expression of pluripotent cells in each horn of uterus suggests the influence of ovarian hormones on these characteristics. The highest mRNA and protein expression for pluripotent markers was observed in stromal cells among uterine cells, which indicates this population of cells as the main site of pluripotent cells in the cow uterus.

show abstract

Section: Discussionsupporting

confidence: 89%

Identification of pluripotent cells in bovine uterus: in situ and in vitro studies

Łupicka

Bodek

Shpigel³

et al. 2015

Reproduction

View full text Add to dashboard Cite

show abstract

“…Similar clonogenic efficiency has been found in early and late luteal phase of bovine oestrous cycle (Cabezas et al., ). Studies in porcine uterus and human endometrium have shown that the clonogenic activity of cells does not vary between phases of the cycle, suggesting that these cells are ovarian steroid‐independent hormones (Miernik & Karasinski, ; Schwab, Chan, & Gargett, ).…”

Section: Discussionmentioning

confidence: 99%

Characterization of mesenchymal stem cells in bovine endometrium during follicular phase of oestrous cycle

Lara

Rivera

Rojas

et al. 2017

Reprod Domestic Animals

View full text Add to dashboard Cite

Stem cells have been postulated as responsible for cell regeneration in highly and continuously regenerative tissues such as the endometrium. Few studies in cattle have identified and specified the presence of stem cells in the endometrium during the oestrous cycle. The aim of this study was to investigate the presence of mesenchymal stem cells (MSCs) in the bovine endometrium during the follicular phase (FP) of the oestrous cycle. Uterine tissue was collected in the time-frame comprising day 18 of the cycle and ovulation (day 0). We isolated, cultured and expanded four primary cell lines from endometrium and identified byRT-qPCR the expression of OCT4, SOX2 but not NANOG (undifferentiated/embryonic markers), CD44 (MSCs marker) and c-KIT (stem cell marker) genes; and the encoded Oct4, Sox2 and Cd44 proteins by Western blot or immunostaining of paraffin-embedded tissue in endometrium. We demonstrated that cells isolated from bovine endometrium displayed essentially the same gene expression pattern; however, at the protein level, Oct4 and Cd44 were not detected. Besides, they showed typical functional characteristics of MSCs such as fibroblast-like morphology, plastic adherence, high proliferative capacity, clone formation in vitro and the ability to differentiate into chondrogenic, osteogenic and adipogenic lineages. We obtained for the first time an extensive characterization of undifferentiated cells populations contained in the bovine endometrium during the FP of the oestrous cycle.

show abstract

“…Bone marrow, the most commonly studied source of MSC has been extensively reported since beginning [14-18]. Moreover, MSCs from other sources like umbilical cord blood [19], Wharton’s jelly [20-23], peripheral blood [24], amniotic fluid [25], endometrium [26, 27], neural progenitors [28], retinal progenitors [29], skin [30-32], synovial fluid [33-35] and aorta tunica media [36] have also been isolated successfully. MSCs from these sources exhibit stem cell properties like plastic adherence, multilineage differentiation, expression of CD markers and pluripotent genes.…”

Section: Characteristics Of Mscsmentioning

confidence: 99%

Research Advancements in Porcine Derived Mesenchymal Stem Cells

Bharti¹,

Shivakumar²,

Subbarao³

et al. 2016

CSCR

View full text Add to dashboard Cite

In the present era of stem cell biology, various animals such as Mouse, Bovine, Rabbit and Porcine have been tested for the efficiency of their mesenchymal stem cells (MSCs) before their actual use for stem cell based application in humans. Among them pigs have many similarities to humans in the form of organ size, physiology and their functioning, therefore they have been considered as a valuable model system for in vitro studies and preclinical assessments. Easy assessability, few ethical issues, successful MSC isolation from different origins like bone marrow, skin, umbilical cord blood, Wharton’s jelly, endometrium, amniotic fluid and peripheral blood make porcine a good model for stem cell therapy. Porcine derived MSCs (pMSCs) have shown greater in vitro differentiation and transdifferention potential towards mesenchymal lineages and specialized lineages such as cardiomyocytes, neurons, hepatocytes and pancreatic beta cells. Immunomodulatory and low immunogenic profiles as shown by autologous and heterologous MSCs proves them safe and appropriate models for xenotransplantation purposes. Furthermore, tissue engineered stem cell constructs can be of immense importance in relation to various osteochondral defects which are difficult to treat otherwise. Using pMSCs successful treatment of various disorders like Parkinson’s disease, cardiac ischemia, hepatic failure, has been reported by many studies. Here, in this review we highlight current research findings in the area of porcine mesenchymal stem cells dealing with their isolation methods, differentiation ability, transplantation applications and their therapeutic potential towards various diseases.

show abstract

Porcine uterus contains a population of mesenchymal stem cells

Cited by 45 publications

References 39 publications

Identification of pluripotent cells in bovine uterus: in situ and in vitro studies

Identification of pluripotent cells in bovine uterus: in situ and in vitro studies

Characterization of mesenchymal stem cells in bovine endometrium during follicular phase of oestrous cycle

Research Advancements in Porcine Derived Mesenchymal Stem Cells

Contact Info

Product

Resources

About