Porcine Toll-like receptors: Recognition of Salmonella enterica serovar Choleraesuis and influence of polymorphisms

Shinkai, Hiroki; Suzuki, Rintaro; Akiba, Masato; Okumura, Naohiko; Uenishi, Hirohide

doi:10.1016/j.molimm.2011.02.004

Cited by 27 publications

(30 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Furthermore, synonymous mutations in a gene can play a significant role in transcriptional regulation (Sauna and Kimchi-Sarfaty 2011; Sato et al 2012). Thus, similar to Shinkai et al (2011) who demonstrated polymorphisms in TLR5 and TLR2 alter the cellular response to S. Choleraesuis, our results highlight the importance of linking genetic variations that may influence the molecular function of a key transcriptional regulator (TLR4) with Salmonella shedding in swine.…”

Section: Resultssupporting

confidence: 89%

TLR4 single nucleotide polymorphisms (SNPs) associated with Salmonella shedding in pigs

et al. 2014

View full text Add to dashboard Cite

Toll-like receptor 4 (TLR4) is a key factor in the innate immune recognition of lipopolysaccharide (LPS) from Gram-negative bacteria. Previous studies from our group identified differences in the expression profile of TLR4 and genes affected by the TLR4 signaling pathway among pigs that shed varying levels of Salmonella, a Gram-negative bacterium. Therefore, genetic variation in this gene may be involved with the host’s immune response to bacterial infections. The current study screened for single nucleotide polymorphisms (SNPs) in the TLR4 gene and tested their association with Salmonella fecal shedding. Pigs (n = 117) were intranasally challenged at 7 weeks of age with 1 × 109 CFU of S. Typhimurium χ4232 and were classified as low or persistent Salmonella shedders based on the levels of Salmonella being excreted in fecal material. Salmonella fecal shedding was determined by quantitative bacteriology on days 2, 7, 14, and 20/21 post exposure, and the cumulative levels of Salmonella were calculated to identify the low (n = 20) and persistent (n = 20) Salmonella shedder pigs. From those 40 animals, the TLR4 region was sequenced, and 18 single nucleotide polymorphisms (SNPs) in TLR4 were identified. Twelve SNPs have been previously described and six are novel SNPs of which five are in the 5′ untranslated region and one is in intron 2. Single marker association test identified 13 SNPs associated with the qualitative trait of Salmonella fecal shedding, and seven of those SNPs were also associated with a quantitative measurement of fecal shedding (P < 0.05). Using a stepwise regression process, a haplotype composed of SNPs rs80787918 and rs80907449 (P ≤ 4.0 × 10−3) spanning a region of 4.9 Kb was identified, thereby providing additional information of the influence of those SNPs on Salmonella fecal shedding in pigs.Electronic supplementary materialThe online version of this article (doi:10.1007/s13353-014-0199-8) contains supplementary material, which is available to authorized users.

show abstract

Section: Resultssupporting

confidence: 89%

TLR4 single nucleotide polymorphisms (SNPs) associated with Salmonella shedding in pigs

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Several TLR have been shown to be responsible for recognition of Salmonella. Salmonella enterica Choleraesuis is recognized by pig TLR5 and TLR1/2 [35]. TLR5-mediated recognition of Salmonella plays a role in many host species.…”

Section: Resultsmentioning

confidence: 99%

“…TLR5-mediated recognition of Salmonella plays a role in many host species. Recent findings demonstrated that single amino acid exchanges in Salmonella flagellin alter species-specific host response (human, mouse, chicken) [36] as well as the occurrence of SNPs in TLR5 and TLR2 of different pig populations [35]. Beside those receptors TLR4, TLR9 (and/or TLR3) are involved in Salmonella enterica Typhimurium recognition [37].…”

Section: Resultsmentioning

confidence: 99%

Prediction and Comparison of SalmonellaHuman and SalmonellaArabidopsis Interactomes

Schleker

Garcı́a-Garcı́a

Klein‐Seetharaman

et al. 2012

Chemistry & Biodiversity

View full text Add to dashboard Cite

Salmonellosis caused by Salmonella bacteria is a food-borne disease and worldwide health threat causing millions of infections and thousands of deaths every year. This pathogen infects an usually broad range of host organisms including human and plants. A better understanding of the mechanisms of communication between Salmonella and its hosts requires identifying the interactions between Salmonella and host proteins. Protein-protein interactions (PPIs) are the fundamental building blocks of communication. Here we utilize the prediction platform BIANA to obtain the putative Salmonella-human and Salmonella-Arabidopsis interactomes based on sequence and domain similarity to known PPIs. A gold standard list of Salmonella-host PPIs served to validate the quality of the human model. 24,726 and 10,926 PPIs comprising interactions between 38 and 33 Salmonella effectors and virulence factors with 9,740 human and 4,676 Arabidopsis proteins, respectively, were predicted. Putative hub proteins could be identified and parallels between the two interactomes were discovered. This approach can provide insight into possible biological functions of so far uncharacterized proteins. The predicted interactions are available via a web interface which allows filtering of the database according to parameters provided by the user to narrow down the list of suspected interactions. The interactions are available via a webinterface at http://sbi.imim.es/web/SHIPREC.php

show abstract

“…Our explorations of polymorphisms using the cDNA sequences and ESTs presented here have been useful in characterizing the genetic features of pig breeds and populations [18,42]. We have also investigated polymorphisms in genes encoding pattern-recognition receptors [43-46] and have demonstrated that some of the polymorphisms observed so far in commercial pig and wild boar populations truly affect the ligand-recognition ability of the molecules encoded by the genes [47-49]. In addition, many ongoing studies are revealing the potential associations of gene polymorphisms with economically important traits in pigs [50].…”

Section: Resultsmentioning

confidence: 99%

Large-scale sequencing based on full-length-enriched cDNA libraries in pigs: contribution to annotation of the pig genome draft sequence

Uenishi

Morozumi²,

Toki³

et al. 2012

BMC Genomics

Self Cite

View full text Add to dashboard Cite

BackgroundAlong with the draft sequencing of the pig genome, which has been completed by an international consortium, collection of the nucleotide sequences of genes expressed in various tissues and determination of entire cDNA sequences are necessary for investigations of gene function. The sequences of expressed genes are also useful for genome annotation, which is important for isolating the genes responsible for particular traits.ResultsWe performed a large-scale expressed sequence tag (EST) analysis in pigs by using 32 full-length-enriched cDNA libraries derived from 28 kinds of tissues and cells, including seven tissues (brain, cerebellum, colon, hypothalamus, inguinal lymph node, ovary, and spleen) derived from pigs that were cloned from a sow subjected to genome sequencing. We obtained more than 330,000 EST reads from the 5′-ends of the cDNA clones. Comparison with human and bovine gene catalogs revealed that the ESTs corresponded to at least 15,000 genes. cDNA clones representing contigs and singlets generated by assembly of the EST reads were subjected to full-length determination of inserts. We have finished sequencing 31,079 cDNA clones corresponding to more than 12,000 genes. Mapping of the sequences of these cDNA clones on the draft sequence of the pig genome has indicated that the clones are derived from about 15,000 independent loci on the pig genome.ConclusionsESTs and cDNA sequences derived from full-length-enriched libraries are valuable for annotation of the draft sequence of the pig genome. This information will also contribute to the exploration of promoter sequences on the genome and to molecular biology-based analyses in pigs.

show abstract

Porcine Toll-like receptors: Recognition of Salmonella enterica serovar Choleraesuis and influence of polymorphisms

Cited by 27 publications

References 54 publications

TLR4 single nucleotide polymorphisms (SNPs) associated with Salmonella shedding in pigs

TLR4 single nucleotide polymorphisms (SNPs) associated with Salmonella shedding in pigs

Prediction and Comparison of SalmonellaHuman and SalmonellaArabidopsis Interactomes

Large-scale sequencing based on full-length-enriched cDNA libraries in pigs: contribution to annotation of the pig genome draft sequence

Contact Info

Product

Resources

About