Porcine somatic cell nuclear transfer using telomerase reverse transcriptase-transfected mesenchymal stem cells reduces apoptosis induced by replicative senescence

Abstract

Somatic cell nuclear transfer (SCNT) is an essential technique in biomedical research for the production of transgenic animals, conservation of endangered species, and establishment of embryonic stem cells (ESCs) (Lee et al., 2014). SCNT has been used to clone diverse animals such as pigs, mice, cattle, and non-human primates (Gouveia et al., 2020). Since pigs are not only favored for mass production but also due to their anatomical and physiological similarities to humans, for the establishment of disease models, production of bio-organs, and development

Comparative Analysis of Porcine Adipose- and Wharton’s Jelly-Derived Mesenchymal Stem Cells

“…Therefore, the comparison between the two types of MSCs during early and late passages has broadened the scope of understanding the potential applications of these cells based on characteristics such as proliferation rate, differentiation potential, and mitochondrial metabolism. MSCs are very useful cell types that have been used for clinical purposes in animals [ 57 ], humans [ 58 , 59 ], and cultured meat [ 60 ]. Recently, research on fat has gained significance in the field of cultured meat production.…”

CDDO-Me alleviates oxidative stress in human mesenchymal stem cells

“…Despite their unique usefulness, the therapeutic potential has not been consistent, and sometimes no improvement can be seen (Holan et al, 2016;Lohan et al, 2017). Given these difficulties, studies have shown that various chemical, physical, and genetic modification have improved the trophic effects of MSCs, e.g., adhesion, migration, survival, while limiting the senescence during an extended culture period (Jo et al, 2019;Jeon and Rho, 2020;Ocansey et al, 2020). Specifically, unfavorable microenvironment upon being cultured in vitro and being administrated in vivo precludes the clinical uses of cellbased therapeutic products (Li et al, 2019).…”