Porcine Small Intestinal Submucosa Graft for Repair of Anterior Urethral Strictures

Fiala, R.; Vidlář, Aleš; Vrtal, Radovan; Bělej, Kamil; Študent, Vladimír

doi:10.1016/j.eururo.2007.01.099

Cited by 141 publications

(71 citation statements)

References 28 publications

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“…SIS have already been successfully applied both experimentally and clinically for urethroplasties with a short length of urethral defects [7,8]. However, for repair of a long segment of urethra, an acellular matrix alone was often insufficient because of the limited distance over which the body is able to regenerate functional tissue.…”

Section: Discussionmentioning

confidence: 99%

“…Both preclinical [7] and clinical [8] trials have proved that small intestinal submucosa (SIS) as an onlay graft is feasible for urethroplasties with a short length of urethral strictures, which, unfortunately, revealed an increasing failure rate with long urethral defects. Cell-seeded tissue engineering may offer an alternative technique for the reconstruction of long urethral strictures [9,10].…”

Section: Introductionmentioning

confidence: 99%

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Urethral Reconstruction with Small Intestinal Submucosa Seeded with Oral Keratinocytes and TIMP-1 siRNA Transfected Fibroblasts in a Rabbit Model

Guo

Sa²,

Huang³

et al. 2015

Urol Int

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Background: To evaluate the effect of tissue inhibitor of metalloproteinase-1 small interfering RNA (TIMP-1 siRNA) transfected fibroblasts (FB) for urethral reconstruction. Materials and Methods: A ventral urethral mucosal defect was created. Substitution urethroplasty was performed with small intestinal submucosa (SIS) alone (8 rabbits, group 1), autogenic oral keratinocytes (OK)-seeded SIS (8 rabbits, group 2) or autogenic OK and TIMP-1 siRNA transfected FB-seeded SIS (8 rabbits, group 3). At 1 and 6 months after surgery (4 rabbits at each time point), retrograde urethrogram and histologic analysis were performed to evaluate the outcomes of urethroplasty. Results: TIMP-1 siRNA transfected FB decreased the secretion of type I collagen. Under retrograde urethrography, 5 rabbits in group 1, 6 in group 2 and 7 in group 3 maintained a wide urethral caliber. Histologically, inflammation and fibrosis were observed at 6 months in group 1. The speed of urothelium, smooth muscle and vessel regeneration in group 3 was faster than that in group 2. Comparison of smooth muscle-to-collagen ratio, epithelial layers, smooth muscle content and microvessel density among three groups revealed a significant increase (p < 0.05). Conclusions: TIMP-1 siRNA transfected FB could be used as a source of seed cell for urethral tissue engineering and could prevent the proliferation of urethral scar tissue.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Urethral Reconstruction with Small Intestinal Submucosa Seeded with Oral Keratinocytes and TIMP-1 siRNA Transfected Fibroblasts in a Rabbit Model

Guo

Sa²,

Huang³

et al. 2015

Urol Int

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“…Most reports have been focused on treating urethral strictures using SIS. Among them, most results were satisfactory (Table 2) (50)(51)(52)(53)(54)(55)(56). In the report of Fiala et al, fifty patients with urethral strictures received urethroplasty using SIS.…”

Section: Clinical Applicationmentioning

confidence: 99%

Tissue Engineering in Low Urinary Tract Reconstruction

Cao¹,

Xu²

2012

Tissue Regeneration - From Basic Biology to Clinical Application

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“…At a mean follow-up of 31 months, the success rate was 80%. 39 A second study reported 85% success when SIS was used as either an inlay or onlay patch graft with a mean follow-up of 21 months. However, when used ventrally, the outcome was not as favourable, with a failure reported in 6 of 10 adult patients.…”

Section: Urethramentioning

confidence: 99%

Tissue engineering in urology

Matoka

Cheng

2013

CUAJ

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Tissue engineering encompasses a multidisciplinary approach geared toward the development of biological substitutes designed to restore and maintain normal function in diseased or injured tissues. This article reviews the basic technology that is used to generate implantable tissue-engineered grafts in vitro that will exhibit characteristics in vivo consistent with the physiology and function of the equivalent healthy tissue. We also examine the current trends in tissue engineering designed to tailor scaffold construction, promote angiogenesis and identify an optimal seeded cell source. Finally, we describe several currently applied therapeutic modalities that use a tissue-engineered construct. While notable progress has clearly been demonstrated in this emerging field, these efforts have not yet translated into widespread clinical applicability. With continued development and innovation, there is optimism that the tremendous potential of this field will be realized.Can Urol Assoc J 2009;3(5):403-8 Résumé L'ingénierie tissulaire englobe une approche multidisciplinaire axée sur le développement de substituts biologiques en vue de rétablir et de maintenir la fonction normale de tissus lésés. L'article qui suit passe en revue la technologie fondamentale utilisée pour générer des greffons implantables produits par ingénierie in vitro et possédant des caractéristiques in vivo correspondant aux tissus sains équivalents sur les plans physiologique et fonctionnel. Nous examinons également les tendances actuelles en ingénierie tissulaire visant à adapter des échafaudages tissulaires, à promouvoir l'angiogenèse et à dégager une source optimale de cellules implantables. Enfin, nous décrivons plusieurs modalités thérapeu-tiques actuellement mises en application utilisant un échafaudage créé par ingénierie tissulaire. En dépit de progrès remarquables dans ce domaine en effervescence, les efforts déployés ne se sont pas encore traduits par une applicabilité clinique étendue. Des développements et des percées continus permettent d'être optimiste face au potentiel prodigieux de ce domaine.

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Porcine Small Intestinal Submucosa Graft for Repair of Anterior Urethral Strictures

Cited by 141 publications

References 28 publications

Urethral Reconstruction with Small Intestinal Submucosa Seeded with Oral Keratinocytes and TIMP-1 siRNA Transfected Fibroblasts in a Rabbit Model

Urethral Reconstruction with Small Intestinal Submucosa Seeded with Oral Keratinocytes and TIMP-1 siRNA Transfected Fibroblasts in a Rabbit Model

Tissue Engineering in Low Urinary Tract Reconstruction

Tissue engineering in urology

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