Porcine skin gelatin hydrolysate as a dipeptidyl peptidase IV inhibitor improves glycemic control in streptozotocin-induced diabetic rats

In vitro evidence for gut hormone stimulation release and dipeptidyl-peptidase IV inhibitory activity of protein hydrolysate obtained from cuttlefish ( Sepia officinalis ) viscera

“…Therefore, the active GLP-1 is extensively degraded by DPP-IV transmembrane active form anchored to the basal surface of the intestinal epithelial cells and in the luminal surface of the endothelial cells of the mucosal capillaries (Godinho et al, 2015;Mulvihill & Drucker, 2014). In this way, our results could be linked to those obtained in a recent study showing that an ultrafiltration fraction (< 1 kDa) of porcine skin gelatin hydrolysate, that harboring a DPP-IV inhibition activity in vitro, was able to improve glucose tolerance in diabetic rats after daily administration in correlation with the increase of insulin and the GLP-1 plasma level, the inhibition of plasma DPP-IV activity and the decrease of the plasma glucagon level (Huang, Hung, Jao, Tung, & Hsu, 2014). Further works are needed to identify the peptide sequences and the cellular mechanisms involved in bioactivities and to know if active peptides will be able to cross the intestinal barrier.…”

Screening and identification of DPP-IV inhibitory peptides from deer skin hydrolysates by an integrated approach of LC–MS/MS and in silico analysis

“…These two examples both indicate that a greater peptide sequence length increases the DPP-IV inhibitory activity.Therefore, medium length peptides are also a good resource for DPP-IV inhibitory peptides. Porcine skin gelatin hydrolysates (Huang, Hung, Jao, Tung, & Hsu, 2014) and salmon skin gelatin hydrolysates (Hsieh, Wang, Hung, Chen, & Hsu, 2015) have been proven to inhibit plasma DPP-IV in rats. Hyp-containing peptides were detected in human plasma after the ingestion of a collagen hydrolysate and exerted beneficial effects on human health (Shigemura, Kubomura, Sato, & Sato, 2014).…”

Utilisation of the isobole methodology to study dietary peptide–drug and peptide–peptide interactive effects on dipeptidyl peptidase IV (DPP-IV) inhibition

“…In terms of antidiabetic activity, small animal studies have demonstrated that the ingestion of Leu-Pro-Gln-Asn-Ile-Pro-Pro-Leu (β-casein f70-77, DPP-IV IC 50 = 160 μM) or a tryptic β-lactoglobulin hydrolysate containing Val-Ala-Gly-Thr-Trp-Tyr (β-lg f15-20, DPP-IV IC 50 = 35 174 μM) could lower plasma glucose following an oral glucose tolerance test 5,6 . Recently, it was shown that a porcine skin gelatin hydrolysate could inhibit plasma DPP-IV in rats as well as reducing serum glucose in the post-prandial phase 29 . However, little or no data appears to exist on the effect of foods on the 40 pharmacokinetics of Sitagliptin in vivo following food intake 30 .…”