Porcine RING Finger Protein 114 Inhibits Classical Swine Fever Virus Replication via K27-Linked Polyubiquitination of Viral NS4B

Zhang, Yuexiu; Zhang, Huawei; Zheng, Guanglai; Yang, Qian; Yu, Shaoxiong; Wang, Jinghan; Su, Li; Li, Lian-Feng; Qiu, Hua‐Ji

doi:10.1128/jvi.01248-19

Cited by 25 publications

(12 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been suggested that K27-linked ubiquitination of NS3 enhances protein–protein interactions and facilitates the formation of the NS2B3 complex [ 89 ]. However, this is a rather unexpected finding since this non-canonical ubiquitination has been previously shown to inhibit viral replication by promoting the proteasomal degradation of viral proteins from classical swine fever and porcine reproductive and respiratory syndrome viruses [ 90 , 91 ]. Further experiments will be required to shed more light on K27-linked ubiquitination of viral proteins.…”

Section: Lysine 27—a Major Player Of Innate Immunitymentioning

confidence: 99%

Beyond K48 and K63: non-canonical protein ubiquitination

2021

View full text Add to dashboard Cite

Protein ubiquitination has become one of the most extensively studied post-translational modifications. Originally discovered as a critical element in highly regulated proteolysis, ubiquitination is now regarded as essential for many other cellular processes. This results from the unique features of ubiquitin (Ub) and its ability to form various homo- and heterotypic linkage types involving one of the seven different lysine residues or the free amino group located at its N-terminus. While K48- and K63-linked chains are broadly covered in the literature, the other types of chains assembled through K6, K11, K27, K29, and K33 residues deserve equal attention in the light of the latest discoveries. Here, we provide a concise summary of recent advances in the field of these poorly understood Ub linkages and their possible roles in vivo.

show abstract

Section: Lysine 27—a Major Player Of Innate Immunitymentioning

confidence: 99%

Beyond K48 and K63: non-canonical protein ubiquitination

2021

View full text Add to dashboard Cite

show abstract

“…The RING finger protein family is widespread and functionally conserved in eukaryotes, from yeast to animals. The roles implicated in growth, oxidative stresses tolerance, response to the pathogen and signal transduction (Ardley, 2009;An et al, 2017;Zhang et al, 2019). Rfp17 was annotated to be involved in gamete generation in our results.…”

Section: The Key Dmgs Involved In Reproduction Morphology and Visiomentioning

confidence: 78%

Genome-Wide Differential DNA Methylation in Reproductive, Morphological, and Visual System Differences Between Queen Bee and Worker Bee (Apis mellifera)

et al. 2020

View full text Add to dashboard Cite

There are many differences in external morphology and internal physiology between the Apis mellifera queen bee and worker bee, some of which are relevant to beekeeping production. These include reproductive traits, body size, royal jelly secreting properties, and visual system development, among others. The identification of candidate genes that control the differentiation of these traits is critical for selective honeybee breeding programs. In this study, we compared the genomic methylation of queen bee and worker bee larvae at 3, 4, and 5 days of age by whole-genome bisulfite sequencing, and found that the basic characteristics of genomic methylation in queen and worker larvae were the same. There were approximately 49 million cytosines in the Apis larvae genome, of which about 90,000 were methylated. Methylated CpG sites accounted for 99% of the methylated cytosines, and methylation mainly occurred in exons. However, methylation levels of queen and worker larvae showed different trends with age: the methylation level of queen larvae varied with age in an inverted parabola, while the corresponding trend for worker larvae with resembled an exponential curve with a platform. The methylation level of queen larvae was higher than that of worker larvae at 3 days of age, lower than that of worker larvae at 4 days of age, and similar to that of worker larvae at 5 days old. The top 10 differentially methylated genes (DMGs) and 13 caste-specific methylated genes were listed, and correlations with caste determination were speculated. We additionally screened 38 DMGs between queen larvae and worker larvae involved in specific organ differentiation as well as reproduction, morphology, and vision differentiation during caste determination. These genes are potential molecular markers for selective breeding of A. mellifera to improve fecundity, royal jelly production, body size, and foraging, and represent candidate genes for investigating specialized functional segregation during the process of caste differentiation.

show abstract

“…PARP10 is ubiquitinated by RNF114 via K27-linked polyubiquitination, which enhances PARP10 enzymatic activity. More recently, porcine RNF114 was found to inhibit classical swine fever virus (CSFV) replication via the K27-linked polyubiquitination of viral NS4B [36]. These results indicate that RNF114-mediated polyubiquitination is complicated and that RNF114 may regulate substrate stability and activity.…”

Section: Discussionmentioning

confidence: 99%

RNF114 suppresses tumour metastasis through the regulation of PARP10

Zhao

Liang

Li³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background ADP-ribosylation is a multifunctional post-translational modification catalysed by intracellular ADP-ribosyltransferases. Previously, we demonstrated that the mono-ADP-ribosyltransferase PARP10 suppresses tumour metastasis through the negative regulation of Aurora A kinase activity. However, the mechanisms of PARP10 regulation and modification were unclear. Methods Interaction of RNF114 and PARP10 was identified by exogenous and endogenous reciprocal immunoprecipitation (IP) assays and pull-down assays. Ubiquitination of PARP10 by RNF114 was analysed by in vivo ubiquitination assays. Potential effects of ubiquitination on PARP10’s activity was detected by western blots and pull-down assays. Potential role of RNF114 in tumour metastasis were determined by migration and invasion assays and in vivo metastasis assay. Results That E3 ubiquitin ligase RNF114 is a partner of PARP10 was identified by IP assays. The auto-mono-ADP-ribosylation of PARP10 is required for the interaction of RNF114 and PARP10. RNF114 ubiquitinates PARP10 through K27-linked polyubiquitination, which enhances PARP10 enzymatic activity. Moreover, RNF114 deficiency promotes tumour cell migration and tumour metastasis through the regulation of PARP10 and its downstream signalling pathway. Conclusions Our findings identify RNF114 as a novel functional regulator of PARP10 and provide evidence of crosstalk between the components of K27-linked polyubiquitination and mono-ADP ribosylation.

show abstract

Porcine RING Finger Protein 114 Inhibits Classical Swine Fever Virus Replication via K27-Linked Polyubiquitination of Viral NS4B

Cited by 25 publications

References 49 publications

Beyond K48 and K63: non-canonical protein ubiquitination

Beyond K48 and K63: non-canonical protein ubiquitination

Genome-Wide Differential DNA Methylation in Reproductive, Morphological, and Visual System Differences Between Queen Bee and Worker Bee (Apis mellifera)

RNF114 suppresses tumour metastasis through the regulation of PARP10

Contact Info

Product

Resources

About