Porcine platelet lysate as a supplement for animal cell culture

Abstract

A novel supplementation of cell growth media based on a porcine platelet lysate was developed for culture of animal-derived cells. The platelet lysate was produced from porcine blood and contained lysate of platelets and plasma components. It showed satisfactory microbiological integrity and it carried only low amount of endotoxins (<10 EU/mL). The porcine platelet lysate supported well proliferation of Vero (African green monkey transformed kidney epithelial cells), Chinese hamster ovary (CHO) and hybridoma cells comparable to fetal bovine serum (FBS). Platelet lysate shows promise as a viable choice over FBS as it can be produced in large quantities, high lot-to-lot consistency and with an attractive price structure. Furthermore it is a strong alternative to FBS for ethical reasons. It is expected that it can be used as a general supplementation for most animal cells for research studies on the proliferation of cells and their expression of products.

The effects of platelet lysate on maturation, fertilization and embryo development of NMRI mouse oocytes at germinal vesicle stage

“…Anna et al in 2007 and Lohmann et al in 2010 added PL to the cell culture media and the results showed that PL had good potential to support cell culturing and proliferation [22,23]. Pazoki et al in 2014 also used PL in pre-antral follicle culture medium for the first time and the result demonstrated that PL has an acceptable effect on oocyte growth [24].…”

“…In addition, there is some evidence that growth factors, cytokines, IGF and EGF can affect maturation and follicle development [27,28]. In recent years, some studies have been conducted to improve the culture medium by adding different supplements like PL [22][23][24]. In this study, the maturation medium was enriched by different concentration of PL and maturation, fertilization and subsequent development were monitored.…”

“…PL has the components which are necessary for maturation, like EGF, IGF, cytokines and microelements [17][18][19][20][21] and seems to be a good source of these factors for improving maturation and embryo development. Anna et al in 2007 and Lohmann et al in 2010 added PL to the cell culture media and the results showed that PL had good potential to support cell culturing and proliferation [22,23]. Pazoki et al in 2014 also used PL in pre-antral follicle culture medium for the first time and the result demonstrated that PL has an acceptable effect on oocyte growth [24].…”

“…Other studies demonstrated that PL is replete with proteins and microelements along with growth factors such as fibroblast growth factor (FGF), TGFb, PDGF, IGF and EGF as well as attachment factors like fibrinogen and serotonin which are vital for cell culturing and folliculogenesis [20,21]. In recent years, some projects have been carried out using PL as a new additive supplement to improve media for cell culturing, and the results were impressive [22][23][24].…”

Cultivo e irradiação de fibroblastos humanos em meio enriquecido com lisado de plaquetas para obtenção de camada de sustentação em cultura de células de epiderme

“…Tais preocupações, impulsionaram diversos pesquisadores a buscar alternativas aos componentes de origem animal empregados nesses métodos, focando suas atenções principalmente sobre o Soro Fetal Bovino, por ser este uma fonte de nutrientes, presente no meio de cultura de vários tipos celulares (Aldén et al, 2007).…”