Porcine liver vascular bed in Biodur E20 corrosion casts

Eberlová, Lada; Liška, Václav; Mírka, Hynek; Gregor, Tomáš; Tonar, Zbyněk; Pálek, Richard; Skála, Martin; Brůha, Jan; Vyčítal, Ondřej; Kalusová, Kristýna; Haviar, Stanislav; Králíčková, Milena; Lametschwandtner, Alois

doi:10.5603/fm.a2015.0094

Cited by 14 publications

(24 citation statements)

References 16 publications

(21 reference statements)

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“…Resins that cure to more resilient, flexible types of casts are for instance Biodur E20 Plus (Biodur Products GmbH) or PU4ii (VasQtec). Biodur E20 Plus is an epoxy‐based resin with a viscosity of about 170–190 mPas (Eberlova et al, ) that can be used to cast similar types of specimens as for the Batson's #17 kit, and to which a specific plasticizer (Biodur AE 20) can be added to even increase the flexibility of the resulting cast. PU4ii, a polyurethane resin, is mainly used in microvascular corrosion cast studies.…”

Section: Choice Of Casting Mediummentioning

confidence: 99%

“…Most often, it is referred to as essential to rinse the system to prevent residual blood to form clots that may obstruct the downstream channels. Often, it is also recommended to add heparin to the rinsing solution for the same reason (Eberlova et al, ). However, in a study performed by Martin‐Orti et al (), no beneficial effects of the anticoagulant or rinsing step on the complete filling of the vascular system could be observed.…”

Section: Principles and Technique Of Corrosion Castingmentioning

confidence: 99%

“…As the polymerization step is initiated as soon as the catalyst is added to the mixture (for Batson's #17 this is the promoter that is added subsequently to the catalyst), all preparations for the casting procedure must be completed prior to this addition (Eberlova et al, ). Typically, the base solution, dilutors, dyes and other components are combined first and rigorously stirred to a homogeneous mixture.…”

Section: Principles and Technique Of Corrosion Castingmentioning

confidence: 99%

“…The choice of casting medium from the wide variety in casting media currently available is mainly determined by the purposes the resulting cast has to serve, the size of the specimen and the modalities of subsequent examination (Eberlova et al, ), whilst the financial aspect is also to be considered. However, the successful outcome of corrosion casting relies on much more than solely the choice of the casting medium.…”

Section: Introduction and Historical Overviewmentioning

confidence: 99%

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Corrosion casting in anatomy: Visualizing the architecture of hollow structures and surface details

Cornillie

Casteleyn

Horst³

et al. 2019

Anat Histol Embryol

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Corrosion casting is the technique by which a solid, negative replica is created from a hollow anatomical structure and liberated from its surrounding tissues. For centuries, different types of hardening substances have been developed to create such casts, but nowadays, thermosetting polymers are mostly used as casting medium. Although the principle and initial set‐up are relatively easy, producing high‐quality casts that serve their intended purpose can be quite challenging. This paper evaluates some of the more popular casting resins that are currently available and provides a step‐by‐step overview of the corrosion casting procedure, including surface casts of anatomical structures. Hurdles and pitfalls are discussed, along with possible solutions to circumvent them, based on personal experience by the authors.

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Section: Choice Of Casting Mediummentioning

confidence: 99%

Section: Principles and Technique Of Corrosion Castingmentioning

confidence: 99%

Section: Principles and Technique Of Corrosion Castingmentioning

confidence: 99%

Section: Introduction and Historical Overviewmentioning

confidence: 99%

See 2 more Smart Citations

Corrosion casting in anatomy: Visualizing the architecture of hollow structures and surface details

Cornillie

Casteleyn

Horst³

et al. 2019

Anat Histol Embryol

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“…Mapping of the volume fractions of the main porcine liver components remains to be performed. As shown in recent studies (Debbaut et al 2012(Debbaut et al , 2014b, this may be done economically by combining the histological approach with a three-dimensional analysis and stereological quantification of hepatic microvessels using high-resolution X-ray microtomography of vascular corrosion casts (Eberlova et al 2016;Ji r ık et al 2016). Moreover, quantitative data regarding the size and density of hepatocytes may also be combined with multilevel modeling of hepatic perfusion (Debbaut et al 2014a;Peeters et al 2015).…”

Section: Distribution Of Larger Mnhep Correlates With a Larger Volumementioning

confidence: 99%

Stereological analysis of size and density of hepatocytes in the porcine liver

et al. 2016

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The porcine liver is frequently used as a large animal model for verification of surgical techniques, as well as experimental therapies. Often, a histological evaluation is required that include measurements of the size, nuclearity or density of hepatocytes. Our aims were to assess the mean number-weighted volume of hepatocytes, the numerical density of hepatocytes, and the fraction of binuclear hepatocytes (BnHEP) in the porcine liver, and compare the distribution of these parameters among hepatic lobes and macroscopic regions of interest (ROIs) with different positions related to the liver vasculature. Using disector and nucleator as design-based stereological methods, the morphometry of hepatocytes was quantified in seven healthy piglets. The samples were obtained from all six hepatic lobes and three ROIs (peripheral, paracaval and paraportal) within each lobe. Histological sections (thickness 16 μm) of formalin-fixed paraffin-embedded material were stained with the periodic acid-Schiff reaction to indicate the cell outlines and were assessed in a series of 3-μm-thick optical sections. The mean number-weighted volume of mononuclear hepatocytes (MnHEP) in all samples was 3670 ± 805 μm (mean ± SD). The mean number-weighted volume of BnHEP was 7050 ± 2550 μm . The fraction of BnHEP was 4 ± 2%. The numerical density of all hepatocytes was 146 997 ± 15 738 cells mm of liver parenchyma. The porcine hepatic lobes contained hepatocytes of a comparable size, nuclearity and density. No significant differences were identified between the lobes. The peripheral ROIs of the hepatic lobes contained the largest MnHEP with the smallest numerical density. The distribution of a larger MnHEP was correlated with a larger volume of BnHEP and a smaller numerical density of all hepatocytes. Practical recommendations for designing studies that involve stereological evaluations of the size, nuclearity and density of hepatocytes in porcine liver are provided.

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From identifying polymeric resins to corrosion casting applications

Reyes

D’hooge

Cornillie

2022

J of Applied Polymer Sci

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Corrosion casting is an important manufacturing technique to replicate anatomical structures. Its execution is often based on the injection of resins that can be polymerized starting at room temperature in the presence of an accelerator, which suits most of the practical requirements. In the present contribution, we highlight the main challenges of the corrosion casting technique, addressing a multidisciplinary research angle connecting the fields of life science, veterinary science, materials science, rheology, and polymer reaction engineering. We pay attention to (commercial) material availability, casting medium selection criteria, specimen preparation, maceration, injection possibilities, cleaning procedures, and detail of replication. We start the discussion from general principles and recommendations to then include major examples for each corrosion casting characteristic addressed.

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Porcine liver vascular bed in Biodur E20 corrosion casts

Abstract: Background: Pigs are frequently used as animal models in experimental medicine. To identify processes of vascular development or regression, vascular elements

Cited by 14 publications

References 16 publications

Corrosion casting in anatomy: Visualizing the architecture of hollow structures and surface details

Corrosion casting in anatomy: Visualizing the architecture of hollow structures and surface details

Stereological analysis of size and density of hepatocytes in the porcine liver

From identifying polymeric resins to corrosion casting applications

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