Porcine intact and wounded skin responses to atmospheric nonthermal plasma

Wu, Andrew S.; Kalghatgi, Sameer; Dobrynin, Danil; Sensenig, Rachel; Cerchar, Ekaternia; Podolsky, Erica R.; Dulaimi, Essel; Paff, Michelle; Wasko, Kimberly; Arjunan, Krishna Priya; Garcia, Kristin; Fridman, Gregory; Balasubramanian, Manjula; Ownbey, Robert T.; Barbee, Kenneth A.; Fridman, Alexander; Friedman, Gary D.; Joshi, Suresh G.; Brooks, Ari D.

doi:10.1016/j.jss.2012.02.039

Cited by 71 publications

(57 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…At high plasma power density of 1.4 W/cm 2 , this FE-DBD device was capable of killing melanoma cells after 15 s treatment time [27]. In a further study, it has been shown that this device, depending on parameters such as plasma-exposure time and power, can damage intact porcine skin [28], clearly demonstrating the need for safety assessments when using DBD devices in medical applications.…”

Section: Introductionmentioning

confidence: 86%

The topical use of non-thermal dielectric barrier discharge (DBD): Nitric oxide related effects on human skin

et al. 2015

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 86%

The topical use of non-thermal dielectric barrier discharge (DBD): Nitric oxide related effects on human skin

et al. 2015

View full text Add to dashboard Cite

“…Wound healing is influenced by many factors including blood coagulation [1], inflammation [2], fibroplasia [3], collagen deposition [4] and wound contraction [5]. In recent years, novel designed wound dressing have been suggested to accelerate wound healing.…”

Section: Introductionmentioning

confidence: 99%

Solution blowing of chitosan/PLA/PEG hydrogel nanofibers for wound dressing

Zhou

et al. 2016

Fibers Polym

View full text Add to dashboard Cite

In this study, a kind of hydrogel nanofibers were successfully fabricated via solution blowing of chitosan (CS) and polylactic acid (PLA) solutions mixed with various contents of polyethylene glycol (PEG) to offer hydration. The nanofibers with PEG content varying were average 341-376 nm in diameter with smooth surface and distributed randomly forming three-dimension (3D) mats. Glutaraldehyde (GA) vapor was then applied to impart stability, and the cross-linking reaction mainly occurred between GA and hydroxyl groups which was confirmed by XPS. The hydrogel nanofibers showed quick absorption behavior, high equilibrate water absorption and good air permeability which could help the mats absorbing excess exudates, creating a moist wound healing environment and oxygen exchanging in wound healing. The mats also exhibited good antibacterial activities against E. coil. The combination advantages of nanofibers mats and hydrogel will help it find promising application in wound healing.

show abstract

“…Von Woedtke et al 18 classified plasma for medical application according to two general principles: indirect use of plasma to treat surfaces, materials, or devices for medical applications, and application of physical plasma on or within the human body. Sterilization of medical materials or devices (e.g., medical implants, catheters, or materials in blood purification systems) is the main use of indirect plasmas for medical purposes; 20 whereas physical plasmas are under intense study for applications in wound healing, 19,20 blood coagulation and skin regeneration, 21 dentistry, 22 and apoptosis of cancer cells. 23 To date, research on ACP sterilization has demonstrated effective reduction of clinically important microorganisms in their planktonic state or in biofilms.…”

Section: Introductionmentioning

confidence: 99%

Dielectric Barrier Discharge Atmospheric Cold Plasma for Inactivation of Pseudomonas aeruginosa Biofilms

Ziuzina¹,

Patil²,

Cullen

et al. 2014

Plasma Med

View full text Add to dashboard Cite

ABSTRACT:In recent years, atmospheric cold plasma (ACP) has been widely investigated for potential application as an alternative decontamination technology in biomedical and healthcare sectors. In this study, the antimicrobial efficacy of ACP against Pseudomonas aeruginosa biofilms was investigated. The 48-h biofilms were treated inside sealed polypropylene containers with a high-voltage dielectric barrier discharge (DBD) ACP (80 kV RMS ) and subsequently stored for 24 h at room temperature. Treatment for 60 s by either the direct or indirect mode of ACP exposure (inside or outside plasma discharge, respectively) reduced bacterial populations by an average of 5.4 log cycles from an initial 6.6 log 10 CFU/mL. Increasing the treatment time from 60 s to 120 s and 300 s reduced biofilms to undetectable levels. According to XTT assay (a metabolic activity assay), an extended treatment time of 300 s was necessary to reduce metabolic activity of cells in biofilms by an average of 70%. Further investigation of biofilm viability by confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM) demonstrated that extended ACP treatment had a detrimental effect on the viability of P. aeruginosa through disintegration of both bacterial cells and the biofilm matrix. The results of this study demonstrate the potential of a novel, in-package, high-voltage ACP decontamination approach for the inactivation of bacterial biofilms.

show abstract

Porcine intact and wounded skin responses to atmospheric nonthermal plasma

Cited by 71 publications

References 18 publications

The topical use of non-thermal dielectric barrier discharge (DBD): Nitric oxide related effects on human skin

The topical use of non-thermal dielectric barrier discharge (DBD): Nitric oxide related effects on human skin

Solution blowing of chitosan/PLA/PEG hydrogel nanofibers for wound dressing

Dielectric Barrier Discharge Atmospheric Cold Plasma for Inactivation of Pseudomonas aeruginosa Biofilms

Contact Info

Product

Resources

About