Porcine hepatocyte isolation and reversible immortalization mediated by retroviral transfer and site-specific recombination

Meng, Fan; Chen, Zhi Shui; Han, Meng; Hu, Xin; He, Xia; Liu, Yong; We, He; Huang, Wei; Guo, Hui; Zhou, Ping

doi:10.3748/wjg.v16.i13.1660

Cited by 28 publications

(36 citation statements)

References 22 publications

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“…15 The commonly used acupoint in treating gastrointestinal diseases is Zusanli (ST36). 16,17 ST36 is located on the Stomach Meridian and its action, harmonizes qi and blood, adjusts the spleen and stomach, and improves general weakness; its traditional therapeutic properties are ideally suited to treating POI. 18 Electroacupuncture at ST36 at a specific frequency can improve gastrointestinal functional diseases.…”

Section: Introductionmentioning

confidence: 99%

Zusanli (ST36) acupoint injection for preventing postoperative ileus: A systematic review and meta-analysis of randomized clinical trials

Wang¹,

Yun-hai²,

Xu³

et al. 2015

Complementary Therapies in Medicine

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Summary Objective To evaluate the preventive effect of Zusanli (ST36) acupoint injections with various agents, for postoperative ileus (POI). Methods We searched electronic databases for randomized controlled trials from inception to 1st February 2015 evaluating ST36 acupoint injection for preventing POI. Revman 5.2.0 was used for data analysis with effect estimates presented as mean difference (MD) with 95% confidence interval (CI). Statistical heterogeneity was tested using I2 (defined as significant if I2 > 75%). We used a random effects model (REM) for pooling data with significant heterogeneity. Results Thirty trials involving 2967 participants were included. All trials were assessed as high risk of bias (poor methodological quality). For time to first flatus, meta-analysis favored ST36 acupoint injection of neostigmine (MD −20.70 h, 95% CI −25.53 to −15.87, 15 trials, I2 = 98%, REM), vitamin B1 (MD −11.22 h, 95% CI −17.01 to −5.43, 5 trials, I2 = 98%, REM), and metoclopramide (MD −15.65 h, 95% CI −24.77 to −6.53, 3 trials, I2 = 94%, REM) compared to usual care alone. Meta-analysis of vitamin B1 favored ST36 acupoint injection compared to intra-muscular injection (MD −17.21 h, 95% CI −21.05 to −13.36, 4 trials, I2 = 89%, REM). Similarly, for time to bowel sounds recovery and first defecation, ST36 acupoint injection also showed positive effects. Conclusions ST36 acupoint injections with various agents may have a preventive effect for POI. Safety is inconclusive as few of included trials reported adverse events. Due to the poor methodological quality and likely publication bias further robust clinical trials are required to arrive at a definitive conclusion.

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Section: Introductionmentioning

confidence: 99%

Zusanli (ST36) acupoint injection for preventing postoperative ileus: A systematic review and meta-analysis of randomized clinical trials

Wang¹,

Yun-hai²,

Xu³

et al. 2015

Complementary Therapies in Medicine

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“…Acupuncture improves GI motility in laparotomy animal models [12131415] and has a positive effect on GI motility in healthy volunteers, patients with diabetic gastroparesis, and those who have undergone transabdominal hysterectomy and colonic surgery [161718192021]. However, the role of acupuncture for reducing POI is less clear, and data from Chinese and Western literature are scarce.…”

Section: Discussionmentioning

confidence: 99%

Effect of Acupuncture on Postoperative Ileus after Distal Gastrectomy for Gastric Cancer

et al. 2017

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PurposeAcupuncture has recently been accepted as a treatment option for managing postoperative ileus (POI) and various functional gastrointestinal disorders. Therefore, we conducted a prospective randomized study to evaluate the effect of acupuncture on POI and other surgical outcomes in patients who underwent gastric surgery.Materials and MethodsThirty-six patients who underwent distal gastrectomy for gastric cancer from March to December 2015 were randomly assigned to acupuncture or non-acupuncture (NA) groups at 1:1 ratio. The acupuncture treatment was administered treatment once daily for 5 consecutive days starting at postoperative day 1. The primary outcome measure was the number of remnant sitz markers in the small intestine on abdominal radiograph. The secondary outcome measure was the surgical outcome, including the times to first flatus, first defecation, start of water intake, and start of soft diet, as well as length of hospital stay and laboratory findings.ResultsThe acupuncture group had significantly fewer remnant sitz markers in the small intestine on postoperative days 3 and 5 compared to those in the NA group. A significant difference was observed in the numbers of remnant sitz markers in the small intestine with respect to group differences by time (P<0.0001). The acupuncture group showed relatively better surgical outcomes than those in the NA group, but the differences were not statistically significant.ConclusionsIn this clinical trial, acupuncture promoted the passage of sitz markers, which may reflect the possibility of reducing POI after distal gastrectomy.

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“…Following transfection with the pCAGMerCreMer/Puro R , a resultant clone, 16-T3, was isolated for further studies based on the Cre/LoxP recombination efficiency. Reversible immortalization of rat and porcine hepatocytes has also been reported [18,19].…”

Section: Reversibly Immortalized Human Hepatocytesmentioning

confidence: 92%

Controlled Expansion of Mammalian Cell Populations by Reversible Immortalization

Noguchi¹,

Kobayashi²

2013

J Biotechnol Biomaterial

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In 1996, reversible immortalization using SV40 large T antigens and the Cre/LoxP system was successfully achieved with primary human fibroblasts. The concept of reversible immortalization involves introducing an immortalizing agent, SV40 large T antigens, into primary cells, expanding the cells in the culture, and finally, efficiently removing the immortalizing agent using Cre/LoxP site-specific recombination. The resulting cell population is essentially identical to the initial primary cells, but greatly increased in number. Since this report, reversible immortalization has been realized with hepatocytes, pancreatic β-cells, hepatic stellate cells, endothelial cells, renal epithelial cells and myogenic cells. This method facilitates the study of cell transplantation as well as cell differentiation, the cell cycle and senescence, by allowing one to control cell proliferation.

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Porcine hepatocyte isolation and reversible immortalization mediated by retroviral transfer and site-specific recombination

Abstract: Acupuncture did not prevent PPOI and was not useful for treating PPOI once it had developed in this population.

Cited by 28 publications

References 22 publications

Zusanli (ST36) acupoint injection for preventing postoperative ileus: A systematic review and meta-analysis of randomized clinical trials

Zusanli (ST36) acupoint injection for preventing postoperative ileus: A systematic review and meta-analysis of randomized clinical trials

Effect of Acupuncture on Postoperative Ileus after Distal Gastrectomy for Gastric Cancer

Controlled Expansion of Mammalian Cell Populations by Reversible Immortalization

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