Porcine extracellular matrix scaffolds in reconstructive urology: An ex vivo comparative study of their biomechanical properties

Davis, Niall F.; Callanan, Anthony; McGuire, Barry B.; Mooney, Rory; Flood, Hugh D.; McGloughlin, Timothy M.

doi:10.1016/j.jmbbm.2010.11.005

Cited by 18 publications

(20 citation statements)

References 27 publications

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“…The mechanical properties of multilayered UBM have been investigated and our tensile-testing findings are consistent with results from prior studies [4,13,23]. Interestingly, researchers have previously attempted to manipulate the material properties of UBM for different surgical subspecialties by varying the number of layers (from 4 to 8) and by modifying the sterilisation treatments used for manufacturing [13].…”

Section: Discussionsupporting

confidence: 80%

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Mechanical characterisation of unidirectional and cross-directional multilayered urinary bladder matrix (UBM) scaffolds

Callanan

Davis

Walsh

et al. 2012

Medical Engineering & Physics

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Section: Discussionsupporting

confidence: 80%

“…Typically, ECMs are prepared from porcine organs as porcine mammalian models are easily sourced and possess similar physiological parameters to humans [4]. Initially, the porcine organ is manually harvested post mortem and subjected to thorough preparation techniques prior to surgical implantation.…”

Section: Introductionmentioning

confidence: 99%

Mechanical characterisation of unidirectional and cross-directional multilayered urinary bladder matrix (UBM) scaffolds

Callanan

Davis

Walsh

et al. 2012

Medical Engineering & Physics

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“…The resulting viscoelastic parameters (E 1 , E 2 , and g) were significantly higher for the decellularized pcECM than for the native tissue, implying an increased stiffness and apparent viscosity due to decellularization, which is also consistent with the measured increase in the Young's modulus. This increased stiffness, previously reported elsewhere, 7,10,12,55 and demonstrated herein for the decellularized pcECM using both analysis methods, can possibly be attributed to a denser and more compact ECM mainly composed of collagen fibers 10,46,56 as a result of the loss of cellular components. Yet the toe border strain (presented in the Supplementary Data) was similar for both the pcECM and native tissue, implying that the preservation of collagen and nonload-bearing proteins, 41,56 such as elastin, is also consistent with our previously reported results showing a minimal loss of such ECM components during decellularization.…”

Section: Discussionsupporting

confidence: 80%

A Mathematical Model for Analyzing the Elasticity, Viscosity, and Failure of Soft Tissue: Comparison of Native and Decellularized Porcine Cardiac Extracellular Matrix for Tissue Engineering

Bronshtein

Au-Yeung

Sarig

et al. 2013

Tissue Engineering Part C: Methods

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The clinical success of tissue-engineered constructs commonly requires mechanical properties that closely mimic those of the human tissue. Determining the viscoelastic properties of such biomaterials and the factors governing their failure profiles, however, has proven challenging, although collecting extensive data regarding their tensile behavior is straightforward. The easily calculated Young's modulus remains the most reported mechanical measure, regardless of its limitations, even though single-relaxation-time (SRT) models can provide much more information, which remain scarce due to a lack of manageable tools for implementing these models. We developed an easy-to-use algorithm for applying the Zener SRT model and determining the elastic moduli, viscosity, and failure profiles of materials under different mechanical tests in a user-independent manner. The algorithm was validated on the data resulting from tensile tests on native and decellularized porcine cardiac tissue, previously suggested as a promising scaffold material for cardiac tissue engineering. This analysis yields new and more accurate measurements such as the elastic moduli and viscosity, the model's relaxation time, and information on the factors governing the materials' failure profiles. These measurements indicate that the viscoelasticity and strength of the decellularized acellular extracellular matrix (ECM) are similar to those of native tissue, although its elasticity and apparent viscosity are higher. Nonetheless, reseeding and culturing the ECM with mesenchymal stem cells was shown to partially restore the mechanical properties lost after decellularization. We propose this algorithm as a platform for soft-tissue analysis that can provide comparable and unbiased measures for characterizing viscoelastic biomaterials commonly used in tissue engineering.

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“…Color images available online at www.liebertonline.com/tea rived from the porcine urinary bladder has been used in multiple pre-clinical applications for site-specific remodeling of a variety of soft tissues. [54][55][56][57][58][59][60][61][62][63] However, commercially available biologic scaffolds composed of ECM are derived from a number of species and organs. 64 It is possible, and in fact likely, that a similar cryptic peptide would be found in ECMs from other sources.…”

Section: Figmentioning

confidence: 99%

Recruitment of Progenitor Cells by an Extracellular Matrix Cryptic Peptide in a Mouse Model of Digit Amputation

Agrawal

Tottey

Johnson

et al. 2011

Tissue Engineering Part A

159

136

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Biologic scaffolds composed of extracellular matrix (ECM) have been used successfully in preclinical models and humans for constructive remodeling of functional, site-appropriate tissue after injury. The mechanisms underlying ECM-mediated constructive remodeling are not completely understood, but scaffold degradation and sitedirected recruitment of both differentiated and progenitor cells are thought to play critical roles. Previous studies have shown that degradation products of ECM scaffolds can recruit a population of progenitor cells both in vitro and in vivo. The present study identified a single cryptic peptide derived from the a subunit of the collagen III molecule that is chemotactic for a well-characterized perivascular stem cell in vitro and causes the site-directed accumulation of progenitor cells in vivo. The oligopeptide was additionally chemotactic for human cortical neural stem cells, rat adipocyte stem cells, C2C12 myoblast cells, and rat Schwann cells in vitro. In an adult murine model of digit amputation, treatment with this peptide after mid-second phalanx amputation resulted in a greater number of Sox2 + and Sca1 + ,Lin -cells at the site of injury compared to controls. Since progenitor cell activation and recruitment are key prerequisites for epimorphic regeneration in adult mammalian tissues, endogenous site-directed recruitment of such cells has the potential to alter the default wound healing response from scar tissue toward regeneration.

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Porcine extracellular matrix scaffolds in reconstructive urology: An ex vivo comparative study of their biomechanical properties

Cited by 18 publications

References 27 publications

Mechanical characterisation of unidirectional and cross-directional multilayered urinary bladder matrix (UBM) scaffolds

Mechanical characterisation of unidirectional and cross-directional multilayered urinary bladder matrix (UBM) scaffolds

A Mathematical Model for Analyzing the Elasticity, Viscosity, and Failure of Soft Tissue: Comparison of Native and Decellularized Porcine Cardiac Extracellular Matrix for Tissue Engineering

Recruitment of Progenitor Cells by an Extracellular Matrix Cryptic Peptide in a Mouse Model of Digit Amputation

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