Porcine cumulus cell influences ooplasmic mitochondria–lipid distributions, GSH–ATP contents and calcium release pattern after electro-activation

Cui, Maosheng; Yang, Fan; Wu, Yue; Hao, Ze-Dong; Liu, S.; Chen, X.J.; Zeng, Shenming

doi:10.1016/j.theriogenology.2008.08.008

Cited by 24 publications

(24 citation statements)

References 48 publications

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“…Denuded mature oocytes unquestionably present differences in Ca 2+ homeostasis. In fact, the duration of Ca 2+ rise was reported to be higher but with lower amplitude in denuded mature pig oocytes compared with those matured in the presence of CC: COC or denuded oocytes cultured with CC added to culture medium [17]. Also, the activation of denuded mature oocytes mediated through Ca 2+ peaks seems to be hampered, interfering with cytoskeleton and organelles migration, namely, LD and cortical granules, with repercussions in membrane block to polyspermy.…”

Section: Oocyte Quality and Developmental Competence Acquisitionmentioning

confidence: 99%

“…This CC expression profile can be useful as predictors of oocyte quality [3, 15]. Furthermore, the simultaneous expansion of compact layers of CC surrounding the oocyte and deposition of mucoelastic material in the extracellular matrix is implicated in supporting both the nuclear maturation and the cytoplasmic maturation [3, 17, 19]. The beneficial effect of CC during oocyte growth to stimulate competence acquisition to further support embryonic development is therefore unequivocal.…”

Section: Oocyte Quality and Developmental Competence Acquisitionmentioning

confidence: 99%

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A Role of Lipid Metabolism during Cumulus-Oocyte Complex Maturation: Impact of Lipid Modulators to Improve Embryo Production

Prates

Nunes

Pereira

2014

Mediators of Inflammation

115

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Oocyte intracellular lipids are mainly stored in lipid droplets (LD) providing energy for proper growth and development. Lipids are also important signalling molecules involved in the regulatory mechanisms of maturation and hence in oocyte competence acquisition. Recent studies show that LD are highly dynamic organelles. They change their shape, volume, and location within the ooplasm as well as their interaction with other organelles during the maturation process. The droplets high lipid content has been correlated with impaired oocyte developmental competence and low cryosurvival. Yet the underlying mechanisms are not fully understood. In particular, the lipid-rich pig oocyte might be an excellent model to understand the role of lipids and fatty acid metabolism during the mammalian oocyte maturation and their implications on subsequent monospermic fertilization and preimplantation embryo development. The possibility of using chemical molecules to modulate the lipid content of oocytes and embryos to improve cryopreservation as well as its biological effects during development is here described. Furthermore, these principles of lipid content modulation may be applied not only to germ cells and embryo cryopreservation in livestock production but also to biomedical fundamental research.

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Section: Oocyte Quality and Developmental Competence Acquisitionmentioning

confidence: 99%

Section: Oocyte Quality and Developmental Competence Acquisitionmentioning

confidence: 99%

A Role of Lipid Metabolism during Cumulus-Oocyte Complex Maturation: Impact of Lipid Modulators to Improve Embryo Production

Prates

Nunes

Pereira

2014

Mediators of Inflammation

115

View full text Add to dashboard Cite

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“…In this respect, the composition of IVM medium to support recovery processes may be important. Also it is highly plausible that presence of cumulus cells and maintaining cumulus functions (e.g., gap junctional communications between cumulus cells and oocyte) is indispensable for this process because cumulus cells and the junctions have basic importance for porcine oocytes to acquire developmental competence and normal mitochondrial functions during maturation [68,69]. In addition, the timing of vitrification during the GV stage seems to have a huge impact on porcine oocytes to regain their meiotic competence.…”

Section: Immature Oocytes; Low Survival But the Developmental Abilitymentioning

confidence: 99%

Factors Affecting Cryopreservation of Porcine Oocytes

2012

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“…Through these pass pyruvate (Paradis, 2009), ATP and glutathione (Cui et al, 2009) and cumulus cells can arrest oocyte meiosis via generating oocyte maturation inhibitors (Chaning et al, 1980). Cumulus cells undergo expansion induced by the pre-ovulatory luteinizing hormone (LH) surge (Assidi et al, 2010).…”

Section: In Vitro Evaluation Of the Reldan 22 ® Insecticide Effects Omentioning

confidence: 99%

In Vitro Testing of the Insecticide Reldan 22 on Swine Oocyte Maturation

Miclea¹,

Cosma²,

Zăhan³

et al. 2016

BUASVMCN-ASB

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Chlorpyrifos (Reldan 22®) is an widely used insecticide for the control of insect pests in agriculture and in residential areas. It is classified as moderately toxic by the United States Environmental Protection Agency and has been quantified in human biological fluids. Our aim was to test the toxicity of Chlorpyrifos (Reldan 22 ® ) and investigate its effects in an in vitro model using swine oocyte maturation. Swine oocytes from ovaries harvested in a commercial slaughterhouse were cultured for 44-45h in M199 supplemented with the following Reldan 22 ® concentrations: 0.1, 0.5, 1 or 2 µl/ml. Cumulus oophorous expansion was assessed and oocytes were denuded and stained with 1 µg/ml fluorescein diacetate to estimate viability. Afterwards, oocytes were fixed in a 60% methanol in DPBS solution and stained with 50 µg/ml propidium iodide to observe the DNA stage. Our research shows that the Reldan 22 ® insecticide stimulated cumulus expansion to an extent but reduced oocyte viability which was accompanied by an increase in the number of immature oocytes and a decrease in the percentages of gametes that resumed meiosis. This leads us conclude that its presence in the oocyte environment is toxic for development at concentrations 0.5, 1 and 2 µl/ml.

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Porcine cumulus cell influences ooplasmic mitochondria–lipid distributions, GSH–ATP contents and calcium release pattern after electro-activation

Cited by 24 publications

References 48 publications

A Role of Lipid Metabolism during Cumulus-Oocyte Complex Maturation: Impact of Lipid Modulators to Improve Embryo Production

A Role of Lipid Metabolism during Cumulus-Oocyte Complex Maturation: Impact of Lipid Modulators to Improve Embryo Production

Factors Affecting Cryopreservation of Porcine Oocytes

In Vitro Testing of the Insecticide Reldan 22 on Swine Oocyte Maturation

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