Porcine circovirus 2 (PCV2) induces a procoagulant state in naturally infected swine and in cultured endothelial cells

Marks, Fernanda Simone; Reck, José; Almeida, Laura Lopes de; Berger, Markus; Corrêa, Ana Grasielle Dionísio; Driemeier, David; Barcellos, David Emílio Santos Neves de; Guimarães, Jorge A.; Termignoni, Carlos; Canal, Cláudio Wageck

doi:10.1016/j.vetmic.2009.08.004

Cited by 16 publications

(24 citation statements)

References 32 publications

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“…4,13,22,23 PCV2 antigen was detected in endothelial cells, smooth muscle cells of the tunica media, infiltrating leukocytes, intravascular and perivascular macrophages, and intravascular monocytes in the present study and in previous reports. 4,11,[13][14][15]17,[21][22][23] Furthermore, intimal spindle cell proliferation was found in a few cases (data not shown) and was recently reported in a cesarean-derived/colostrum-deprived pig inoculated with PCV2b. 13 No correlation was found between the number of infected cells and the presence or severity of the vascular lesions, suggesting a host immune system-induced alteration rather than a direct PCV2-induced lesion.…”

Section: Discussionsupporting

confidence: 55%

Association of Porcine Circovirus Type 2 With Vascular Lesions in Porcine Pneumonia

et al. 2011

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The aim of this study was to evaluate the vasculature in porcine circovirus type 2-infected (PCV2-infected) lungs and to identify the PCV2 subtypes involved in porcine pneumonia. Pulmonary samples from 140 pigs, 2 weeks to 7 months of age, from 36 Hungarian commercial herds with clinical signs of respiratory disease were examined for the presence of respiratory pathogens, with bacterial culture, pathologic evaluation, and immunohistochemistry for PCV2, porcine reproductive respiratory syndrome virus, and swine influenza virus. PCV2 was the most commonly identified pathogen (49 cases) among the 74 of 140 cases (53%) with respiratory pathogens. PCV2 was detected immunohistochemically in the wall of 13% to 100% of pulmonary vessels (mean, 89%) in 38 of 49 cases (78%). Detection of PCV2 antigen was positively correlated with the presence of vascular lesions (P < .001, odds ratio [OR]: 159.54). Other pathogens capable of vascular injury in swine were found in 29 of 49 of the PCV2-positive cases (59%). The probability of detecting vascular lesions in PCV2-infected lung was higher than in infection with porcine reproductive respiratory syndrome virus (P < .002, OR: 14.63), Pasteurella multocida infection (P < .001, OR: 5.75), or Streptococcus spp. infection (not significant, OR: 1.45). Sequence analysis of open reading frame 2 amplicons was possible in 6 PCV2-positive cases, from which 5 cases proved to be PCV2b subtype and 1 case, PCV2a subtype. In conclusion, PCV2 antigen was commonly colocalized with pulmonary vascular lesions in pneumonia in Hungarian swine, and PCV2b was the dominant subtype.

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Section: Discussionsupporting

confidence: 55%

Association of Porcine Circovirus Type 2 With Vascular Lesions in Porcine Pneumonia

et al. 2011

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“…Porcine circovirus 2 (PCV2) belongs to the genus Circovirus of the family Circoviridae (Marks et al, 2010), and is known to cause post-weaning multisystemic wasting syndrome (PMWS) in pigs (Chae, 2005), primarily at 5-16 weeks (Marks et al, 2010). The clinical features of PMWS include progressive weight loss, lymphadenopathy, jaundice and respiratory disease (Chae, 2005), accompanied by increased litter mortality that is significantly related to sow PCV2 status (Calsamiglia et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

TLR2/MyD88/NF-κB signalling pathway regulates IL-8 production in porcine alveolar macrophages infected with porcine circovirus 2

Qin

Qiao

2016

Journal of General Virology

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Porcine circovirus 2 (PCV2) is the primary cause of post-weaning multisystemic wasting syndrome, in which it stimulates a strong IL-8 response that is associated with chronic inflammation as well as lesions in the lymphoid organs. However, the mechanism underlying PCV2-induced IL-8 production is still unclear. In the present study, we demonstrated that increased IL-8 expression during PCV2 infection depends on Toll-like receptor (TLR2), but not TLR4 or TLR9 signalling pathways in porcine alveolar macrophages. Moreover, we found that impairment of the MyD88/NF-kB signalling pathway by MyD88 knockdown or NF-kB inhibitors markedly decreased PCV2-induced IL-8 secretion. These results suggest that PCV2 induces IL-8 secretion via the TLR2/MyD88/NF-kB signalling pathway. Therefore, it is important to elucidate the molecular mechanisms of the PCV2-induced inflammatory response.

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“…In veterinary clinical medicine, some of the topics recently investigated about fibrinogen in farm animals (cattle, horses, pigs, dogs, excluding for the latter two the use as models of human disease; source: PubMed database; period: 2004-09) include: changes related to estrous cycle, pregnancy and parturition [16][17][18][19], liver [20] and heart disease [21][22][23], elective and non-elective surgery [24,25], poisoning/envenomation [26][27][28][29][30], parasite infestation [31][32][33], viral infection [34,35]. The issue most often on focus is however, by far, bacterial infection -especially in respiratory diseases [34,[36][37][38][39][40][41] -and such of its short-or long-term sequelae as septic shock with coagulopathy [42][43][44][45], laminitis [46], or osteomyelitis [47].…”

Section: Introductionmentioning

confidence: 99%

Any use in proteomics for low-tech approaches? Detecting fibrinogen chains of different animal species in two-dimensional electrophoresis patterns

Miller

Gianazza

Gemeiner

2010

Journal of Chromatography B

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Porcine circovirus 2 (PCV2) induces a procoagulant state in naturally infected swine and in cultured endothelial cells

Cited by 16 publications

References 32 publications

Association of Porcine Circovirus Type 2 With Vascular Lesions in Porcine Pneumonia

Association of Porcine Circovirus Type 2 With Vascular Lesions in Porcine Pneumonia

TLR2/MyD88/NF-κB signalling pathway regulates IL-8 production in porcine alveolar macrophages infected with porcine circovirus 2

Any use in proteomics for low-tech approaches? Detecting fibrinogen chains of different animal species in two-dimensional electrophoresis patterns

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