Porcine Bocavirus Infection Associated with Encephalomyelitis in a Pig, Germany1

Pfankuche, Vanessa Maria; Bodewes, Rogier; Hahn, Kerstin; Puff, Christina; Beineke, Andreas; Habierski, A.; Osterhaus, Albert D. M. E.; Baumgärtner, Wolfgang

doi:10.3201/eid2207.152049

Cited by 26 publications

(37 citation statements)

References 10 publications

(11 reference statements)

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“…Whether porcine bocavirus causes disease in pigs is controversial (reviewed in reference 32 ). This virus has been detected in the neurons of a piglet with encephalomyelitis ( 33 ) and has been experimentally shown to interfere with a key interferon signaling pathway ( 34 ); however, the high rate of viral coinfections in pigs has made it difficult to confirm a direct association with disease ( 32 ). Human bocaviruses have also been linked to disease, including pneumonia and other respiratory infections ( 35 , 36 ).…”

Section: Discussionmentioning

confidence: 99%

Viral Diversity of House Mice in New York City

Williams

Che

Garcia

et al. 2018

mBio

104

135

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The microbiome of wild Mus musculus (house mouse), a globally distributed invasive pest that resides in close contact with humans in urban centers, is largely unexplored. Here, we report analysis of the fecal virome of house mice in residential buildings in New York City, NY. Mice were collected at seven sites in Manhattan, Queens, Brooklyn, and the Bronx over a period of 1 year. Unbiased high-throughput sequencing of feces revealed 36 viruses from 18 families and 21 genera, including at least 6 novel viruses and 3 novel genera. A representative screen of 15 viruses by PCR confirmed the presence of 13 of these viruses in liver. We identified an uneven distribution of diversity, with several viruses being associated with specific locations. Higher mouse weight was associated with an increase in the number of viruses detected per mouse, after adjusting for site, sex, and length. We found neither genetic footprints to known human viral pathogens nor antibodies to lymphocytic choriomeningitis virus.

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Section: Discussionmentioning

confidence: 99%

Viral Diversity of House Mice in New York City

Williams

Che

Garcia

et al. 2018

mBio

104

135

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“…It is noteworthy that the closest neighbor of RoBoV is Ungulate bocaparvovirus 4 whose members are all porcine bocaviruses. The first member of Ungulate bocaparvovirus 4 is porcine bocavirus isolate H18 (PBoV-H18) identified in China 10 and other members include isolate KU14 from South Korea 28 , isolate S1142/13 from Germany 29 and isolates JOA011, JOA015 from East Africa 30 . Besides sequence identity, RoBoV possesses some genomic features which have already found in different species of porcine bocavirus.…”

Section: Discussionmentioning

confidence: 99%

Identification and characterization of a novel rodent bocavirus from different rodent species in China

Zhang

Song

Xiu

et al. 2018

Emerging Microbes & Infections

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Members in the genus Bocaparvovirus are closely related to human health and have a wide host range. The diverse hosts raise the possibility of crossing species barrier, which is a feature of emerging viruses. Among the mammalian hosts, rodents are generally acknowledged to be important reservoirs of emerging viruses. Here, rodent samples collected from six provinces and autonomous regions of China (Liaoning, Inner Mongolia, Tibet, Xinjiang, Guangxi and Yunnan) were used to investigate the prevalence and distribution of bocaparvoviruses. By using next-generation sequencing first, a partial non-structural protein 1 (NS1) gene belonging to a possible novel bocaparvovirus was discovered. Following this, PCR-based screening of NS1 gene was conducted in 485 rodent samples, with 106 positive results found in seven rodent species (Rattus norvegicus, Mus musculus, Apodemus agrarius, Cricetulus barabensis, Rattus flavipectus, Rattus rattus and Rhombomys opimus). Finally, six nearly full-length genomes and three complete CDS were obtained and the newly identified bocaparvovirus was tentatively named rodent bocavirus (RoBoV). RoBoV has three ORFs: NS1, NP1, and VP, which are characteristics of bocaparvoviruses. Phylogenetic analyses revealed that porcine bocavirus isolate PBoV-KU14, a member of Ungulate bocaparvovirus 4, was the most related virus to RoBoV, with 92.1–92.9% amino acid identities in NS1 protein. Alignments of RoBoV-related sequences showed RoBoV isolates could be classified into two clades, demonstrating an inter-host genetic diversity. The results indicate a potential interspecies transmission of RoBoV between rodents and swine and expand our knowledge on bocaparvoviruses in rodent populations.

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“…); additionally, PDCoV‐PEDV‐PKV co‐infection also processed certain proportion on PDCoV‐positive samples (20%, 11/55). PKV was also common pathogens causing diarrhoea and viral gastroenteritis in pigs, especially in piglets (Di, Angeloni, Tofani, Monini, & Ruggeri, ; Pfankuche et al., ) and it showed a high co‐infection rate with PEDV (Wang, Lan, & Yang, ; Zhao et al., ). PKV detection has not been included with most other studies of PDCoV; therefore, evidence is lacking to prove that PKV co‐infects more frequently than PEDV with PDCoV.…”

Section: Discussionmentioning

confidence: 99%

The detection and phylogenetic analysis of porcine deltacoronavirus from Guangdong Province in Southern China

Mai

Feng

Chen

et al. 2017

Transbound Emerg Dis

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Porcine deltacoronavirus (PDCoV) is a newly discovered coronavirus that causes diarrhoea, vomiting and dehydration in sucking and nursing piglets. It was first reported in Hong Kong in 2012 and has since been discovered in the United States, Canada, South Korea, mainland China, Thailand and Laos. PDCoV has been experimentally proved to lead to diarrhoea in swine and it was detected positive in pigs in Guangdong, southern China. In our study, 252 faecal and intestinal samples from sucking piglets and sows with diarrhoea were surveyed for common enteric viruses. We found a prevalence of PDCoV (21.8%), porcine epidemic diarrhoea virus (65.5%), transmissible gastroenteritis virus (0%), rotavirus group A (25.0%) and porcine kobuvirus (68.7%). We isolated 13 PDCoV strains and discovered that PDCoV infections were often co-infections with kobuvirus rather than the commonly linked porcine epidemic diarrhoea virus. Phylogenetic analysis of S gene and N gene revealed that 11 of 13 PDCoV strains belonged to Chinese lineage. As for the left two strains, one single strain (CHN-GD16-05) belonged to American and Korean lineages while another strain (CHN-GD16-03) was similar to a Thai strain, but only in the S gene. This suggested a possible recombination event between the Thai and the newly described Chinese strain.

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Porcine Bocavirus Infection Associated with Encephalomyelitis in a Pig, Germany1

Cited by 26 publications

References 10 publications

Viral Diversity of House Mice in New York City

Viral Diversity of House Mice in New York City

Identification and characterization of a novel rodent bocavirus from different rodent species in China

The detection and phylogenetic analysis of porcine deltacoronavirus from Guangdong Province in Southern China

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