Populus Tremula Plants with Reduced Expression of the 4-Coumarate-CoA Ligase Gene Demonstrate Defects of the  Rhizogenesis

Kovalitskaya, Yu. A.; P.Kovalenko, N.; Shestibratov, Konstantin A.

doi:10.14419/ijet.v7i4.36.25374

Cited by 2 publications

(2 citation statements)

References 25 publications

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“…The RT-qPCR method and the analysis of the obtained data were described in detail in Kovalitskaya et al [50]. Actin and ubiquitin were used as reference genes in the study presented here.…”

Section: Real-time Quantitative Pcr (Rt-qpcr) Analysismentioning

confidence: 99%

Various effects of the expression of the xyloglucanase gene from Penicillium canescens in transgenic aspen under semi-natural conditions

et al. 2020

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Background Recombinant carbohydrases genes are used to produce transgenic woody plants with improved phenotypic traits. However, cultivation of such plants in open field is challenging due to a number of problems. Therefore, additional research is needed to alleviate them. Results Results of successful cultivation of the transgenic aspens (Populus tremula) carrying the recombinant xyloglucanase gene (sp-Xeg) from Penicillium canescens in semi-natural conditions are reported in this paper for the first time. Change of carbohydrate composition of wood was observed in transgenic aspens carrying the sp-Xeg gene. The transformed transgenic line Xeg-2-1b demonstrated accelerated growth and increased content of cellulose in wood of trees growing in both greenhouse and outside in comparison with the control untransformed line Pt. The accelerated growth was observed also in the transgenic line Xeg-1-1c. Thicker cell-wall and longer xylem fiber were also observed in both these transgenic lines. Undescribed earlier considerable reduction in the wood decomposition rate of the transgenic aspen stems was also revealed for the transformed transgenic lines. The decomposition rate was approximately twice as lower for the transgenic line Xeg-2-3b in comparison with the control untransformed line Pt. Conclusion A direct dependence of the phenotypic and biochemical traits on the expression of the recombinant gene sp-Xeg was demonstrated. The higher was the level of the sp-Xeg gene expression, the more pronounced were changes in the phenotypic and biochemical traits. All lines showed phenotypic changes in the leave traits. Our results showed that the plants carrying the recombinant sp-Xeg gene do not demonstrate a decrease in growth parameters in semi-natural conditions. In some transgenic lines, a change in the carbohydrate composition of the wood, an increase in the cell wall thickness, and a decrease in the rate of decomposition of wood were observed.

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“…The RT-qPCR method and the analysis of the obtained data were described in detail in Kovalitskaya et al [50]. Actin and ubiquitin were used as reference genes in the study presented here.…”

Section: Real-time Quantitative Pcr (Rt-qpcr) Analysismentioning

confidence: 99%

Various effects of the expression of the xyloglucanase gene from Penicillium canescens in transgenic aspen under semi-natural conditions

et al. 2020

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“…RNA interference-mediated down-regulation of the 4-coumarate: coenzyme A ligase in Populus tremula alters lignification and plant growth [356] and rhizogenesis [357]. Metabolomic studies of these plants revealed strong changes in the biosynthesis of phenolic compounds, in particular the accumulation of glycosylated forms, which may indicate their cytotoxicity.…”

Section: Transgenic Trees and Allelopathymentioning

confidence: 99%

…Fell Upas Sits, the Hydra-Tree of Death †, or the Phytotoxicity of Trees

2019

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The use of natural products that can serve as natural herbicides and insecticides is a promising direction because of their greater safety for humans and environment. Secondary metabolites of plants that are toxic to plants and insects—allelochemicals—can be used as such products. Woody plants can produce allelochemicals, but they are studied much less than herbaceous species. Meanwhile, there is a problem of interaction of woody species with neighboring plants in the process of introduction or invasion, co-cultivation with agricultural crops (agroforestry) or in plantation forestry (multiclonal or multispecies plantations). This review describes woody plants with the greatest allelopathic potential, allelochemicals derived from them, and the prospects for their use as biopesticides. In addition, the achievement of and the prospects for the use of biotechnology methods in relation to the allelopathy of woody plants are presented and discussed.

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Populus Tremula Plants with Reduced Expression of the 4-Coumarate-CoA Ligase Gene Demonstrate Defects of the Rhizogenesis

Cited by 2 publications

References 25 publications

Various effects of the expression of the xyloglucanase gene from Penicillium canescens in transgenic aspen under semi-natural conditions

Various effects of the expression of the xyloglucanase gene from Penicillium canescens in transgenic aspen under semi-natural conditions

…Fell Upas Sits, the Hydra-Tree of Death †, or the Phytotoxicity of Trees

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