Populations of extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae are different in human-polluted environment and food items: a multicentre European study

Martak, Daniel; Guther, Julia; Verschuuren, Tess D.; Valot, Benoı̂t; Conzelmann, Nadine; Bunk, Stefanie; Riccio, María Eugenia; Salamanca, Elena; Meunier, Alexandre; Henriot, Charles P.; Brossier, Caroline; Bertrand, Xavier; Cooper, Ben; Harbarth, Stéphan Juergen; Tacconelli, Evelina; Fluit, Ad C.; Rodríguez‐Baño, Jesús; Kluytmans, Jan; Peter, Silke; Hocquet, Didier

doi:10.1016/j.cmi.2021.07.022

Cited by 22 publications

(13 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is therefore interesting to note that river swimming was the only significant (though rather weak) risk factor associated with 3GC-R E. coli carriage in urban dogs and this may suggest that urban river water in our study region may be contaminated with 3GC-R E. coli, as has been demonstrated in a recent European multicentre study. 28 In conclusion, these analyses suggest interventions that might be used to reduce 3GC-R E. coli carriage in dogs and, in so doing, reduce any resultant zoonotic threat. Limitations of this study include that we did not collect stool samples from dog owners or samples of the raw food these dogs had been fed.…”

Section: Discussionmentioning

confidence: 90%

Molecular ecology and risk factors for third-generation cephalosporin-resistant Escherichia coli carriage by dogs living in urban and nearby rural settings

Sealey

Hammond

Mounsey

et al. 2022

Journal of Antimicrobial Chemotherapy

View full text Add to dashboard Cite

Objectives To compare faecal third-generation cephalosporin-resistant (3GC-R) Escherichia coli isolates from dogs living in a city and in a rural area ∼30 km away; to compare isolates from dogs, cattle and humans in these regions; and to determine risk factors associated with 3GC-R E. coli carriage in these two cohorts of dogs. Methods Six hundred dogs were included, with faecal samples processed to recover 3GC-R E. coli using 2 mg/L cefotaxime. WGS was by Illumina and risk factor analyses were by multivariable linear regression using the results of an owner-completed survey. Results 3GC-R E. coli were excreted by 20/303 rural and 31/297 urban dogs. The dominant canine 3GC-R ST was ST963 (blaCMY-2), which also accounted for 25% of CMY-2-producing E. coli in humans. Phylogenetic overlap between cattle and rural dog CTX-M-14-producing E. coli ST117 was observed as well as acquisition of pMOO-32-positive E. coli ST10 by a rural dog, a plasmid common on cattle farms in the area. Feeding raw meat was associated with carrying 3GC-R E. coli in rural dogs, but not in urban dogs, where swimming in rivers was a weak risk factor. Conclusions Given clear zoonotic potential for resistant canine E. coli, our work suggests interventions that may reduce this threat. In rural dogs, carriage of 3GC-R E. coli, particularly CTX-M producers, was phylogenetically associated with interaction with local cattle and epidemiologically associated with feeding raw meat. In urban dogs, sources of 3GC-R E. coli appear to be more varied and include environments such as rivers.

show abstract

Section: Discussionmentioning

confidence: 90%

Molecular ecology and risk factors for third-generation cephalosporin-resistant Escherichia coli carriage by dogs living in urban and nearby rural settings

Sealey

Hammond

Mounsey

et al. 2022

Journal of Antimicrobial Chemotherapy

View full text Add to dashboard Cite

show abstract

“…4 These reservoirs are clearly interconnected, but the extent of transmission between these reservoirs remains uncertain. [5][6][7] AMR is problematic not only for pathogenic bacteria but also for the commensal intestinal microbiota. The WHO has identified Enterobacterales to be of critical importance, due to the dissemination of ESBLs, cephalosporinases (AmpC) and carbapenemases.…”

Section: Introductionmentioning

confidence: 99%

European-wide antimicrobial resistance monitoring in commensal Escherichia coli isolated from healthy food animals between 2004 and 2018

Jong¹,

Garch²,

Hocquet

et al. 2022

Journal of Antimicrobial Chemotherapy

Self Cite

View full text Add to dashboard Cite

Objectives To describe the susceptibility of Escherichia coli to medically important antibiotics, collected over four periods (2004–2006, 2008–2009, 2013–2014, 2017–2018), from food-producing animals at slaughter. Methods Intestinal contents from cattle, pigs and broilers were randomly sampled (5–6 countries/host; ≥4 abattoirs/country; one sample/animal/farm) for isolation of Escherichia coli; antimicrobial susceptibilities were centrally determined by CLSI agar dilution. Clinical breakpoints (CLSI) and epidemiological cut-off values (EUCAST) were applied for data interpretation. Results In total, 10 613 E. coli strains were recovered. In broilers, resistance percentages were the lowest (P ≤ 0.01) in the latest time period. A significant decrease in MDR over time was also observed for broilers and a tendency for a decrease for pigs. Resistance to meropenem and tigecycline was absent, and resistance to azithromycin was 0.2%–2.0%. Also, low resistance to third-generation cephalosporins (1.1%–7.4%) was detected in broilers. Resistance to colistin varied between 0.1%–4.8%. E. coli from broilers showed high resistance to ciprofloxacin (7.3%–23.3%), whereas for cattle and pigs this was 0.2%–2.5%. Low/moderate resistance to chloramphenicol (9.3%–21.3%) and gentamicin (0.9%–7.0%) was observed in pigs and broilers. The highest resistance was noted for ampicillin (32.7%–65.3%), tetracycline (41.3%–67.5%), trimethoprim (32.0%–35.7%) and trimethoprim/sulfamethoxazole (27.5%–49.7%) from pigs and broilers, with marked country differences. MDR peaked in pigs and broilers with 24 and 26 phenotypes, with 21.9%–26.2% and 18.7%–34.1% resistance, respectively. Conclusions In this pan-EU survey antibiotic susceptibility of commensal E. coli varied largely between antibiotics, animal species and countries. Resistance to critically important antibiotics for human medicine was absent or low, except for ciprofloxacin in broilers and ampicillin in pigs and broilers.

show abstract

“…Furthermore, of the 70-plus countries that have developed national antimicrobial resistance action plans in collaboration with global public health organizations, nearly all have omitted commitments to community-based WASH infrastructure (Essack 2021). This lack of prioritization may reflect a highincome country bias among scientists and policy makers who accept the generalizability of research conducted in settings with strong environmental controls, where the exchange of mobile resistance elements between food animals and the broader community is limited (Day et al 2019;Mughini-Gras et al 2019;Martak et al 2021).…”

Section: Rebalancing Priorities To Combat Antibiotic Resistancementioning

confidence: 99%

“…To date, the importance of environmental controls at the human-animal interface has not received the same level of attention as antibiotic use. This lack of prioritization may be due to high-income country bias, as studies from the UK (Day et al 2019) and the EU (Mughini-Gras et al 2019;Martak et al 2021) have identified only minimal sharing of antibiotic-resistant bacteria between humans and food animals. However, the situation could be vastly different in low-and especially middle-income countries, where environmental controls at the human-animal interface are often deficient or lacking altogether (Figure 1).…”

mentioning

confidence: 99%

Plugging the leaks: antibiotic resistance at human–animal interfaces in low‐resource settings

Nadimpalli,

Stegger,

Viau

et al. 2023

Frontiers in Ecol & Environ

View full text Add to dashboard Cite

Antibiotic resistance is one of the greatest public health challenges of our time. International efforts to curb resistance have largely focused on drug development and limiting unnecessary antibiotic use. However, in areas where water, sanitation, and hygiene infrastructure is lacking, we propose that bacterial flow between humans and animals can exacerbate the emergence and spread of resistant pathogens. Here, we describe the consequences of poor environmental controls by comparing mobile resistance elements among Escherichia coli recovered from humans and meat in Cambodia, a middle‐income country with substantial human–animal connectivity and unregulated antibiotic use. We identified identical mobile resistance elements and a conserved transposon region that were widely dispersed in both humans and animals, a phenomenon rarely observed in high‐income settings. Our findings indicate that plugging leaks at human–animal interfaces should be a critical part of addressing antibiotic resistance in low‐ and especially middle‐income countries.

show abstract

Populations of extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae are different in human-polluted environment and food items: a multicentre European study

Abstract: on behalf of the MODERN WP3 study group, Populations of extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae are different in human-polluted environment and food items: A multicentre European study,

Cited by 22 publications

References 30 publications

Molecular ecology and risk factors for third-generation cephalosporin-resistant Escherichia coli carriage by dogs living in urban and nearby rural settings

Molecular ecology and risk factors for third-generation cephalosporin-resistant Escherichia coli carriage by dogs living in urban and nearby rural settings

European-wide antimicrobial resistance monitoring in commensal Escherichia coli isolated from healthy food animals between 2004 and 2018

Plugging the leaks: antibiotic resistance at human–animal interfaces in low‐resource settings

Contact Info

Product

Resources

About