Population structures shift during the biodegradation of crude and fuel oil by an indigenous consortium

Canul-Chan, M.; Sánchez-González, Mónica Noel; González-Burgos, Araceli; Zepeda, Alejandro; Rojas-Herrera, Rafael

doi:10.1007/s13762-017-1362-7

Cited by 25 publications

(12 citation statements)

References 28 publications

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“…The most abundant OTU, denovo1, was closely related (97% similarity) to Limnobacter litoralis KP1-19 (Lu et al, 2011). Previous work detected the essential genes for aromatic compound degradation in Limnobacter (Vedler et al, 2013), and this microbial group responded to crude oil addition in concurrence with the presence of PAHs (Canul-Chan et al, 2017). The absence of this group in the dispersant treatment may suggest its growth was inhibited by the presence of dispersed oil or an inability to compete with other PAH degraders in dispersed oil.…”

Section: Discussionmentioning

confidence: 94%

Dispersant Enhances Hydrocarbon Degradation and Alters the Structure of Metabolically Active Microbial Communities in Shallow Seawater From the Northeastern Gulf of Mexico

et al. 2019

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Dispersant application is a primary emergency oil spill response strategy and yet the efficacy and unintended consequences of this approach in marine ecosystems remain controversial. To address these uncertainties, ex situ incubations were conducted to quantify the impact of dispersant on petroleum hydrocarbon (PHC) biodegradation rates and microbial community structure at as close as realistically possible to approximated in situ conditions [2 ppm v/v oil with or without dispersant, at a dispersant to oil ratio (DOR) of 1:15] in surface seawater. Biodegradation rates were not substantially affected by dispersant application at low mixing conditions, while under completely dispersed conditions, biodegradation was substantially enhanced, decreasing the overall half-life of total PHC compounds from 15.4 to 8.8 days. While microbial respiration and growth were not substantially altered by dispersant treatment, RNA analysis revealed that dispersant application resulted in pronounced changes to the composition of metabolically active microbial communities, and the abundance of nitrogen-fixing prokaryotes, as determined by qPCR of nitrogenase (nifH) genes, showed a large increase. While the Gammaproteobacteria were enriched in all treatments, the Betaproteobacteria and different families of Alphaproteobacteria predominated in the oil and dispersant treatment, respectively. Results show that mixing conditions regulate the efficacy of dispersant application in an oil slick, and the quantitative increase in the nitrogen-fixing microbial community indicates a selection pressure for nitrogen fixation in response to a readily biodegradable, nitrogen-poor substrate.

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Section: Discussionmentioning

confidence: 94%

Dispersant Enhances Hydrocarbon Degradation and Alters the Structure of Metabolically Active Microbial Communities in Shallow Seawater From the Northeastern Gulf of Mexico

et al. 2019

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“…Furthermore, microbial consortia can be isolated from a specific environment, enriched and stabilized to be used in bioremediation processes (Varjani 2017). Although complex pollutant mixtures can be selectively metabolized by individual micro-organisms, microbial consortia provide a superior metabolic versatility for the degradation of complete assortments of hydrocarbons (Deppe et al 2005;Joshi et al 2014;Canul-Chan et al 2017;Varjani 2017).…”

Section: Discussionmentioning

confidence: 99%

“…Consortium MR-01 has demonstrated the capacity to degrade aromatic molecules like benzene and toluene present in crude oil under aerobic conditions (Canul-Chan et al 2017). Phenol is a highly toxic petroleum derivative; hence, the ability of the consortium MR-01 to degrade this molecule was analysed.…”

Section: Discussionmentioning

confidence: 99%

“…The indigenous consortium MR-01 was isolated from oil-contaminated soil. This consortium was able to degrade 59% of crude oil components after 8 days and 34% of fuel oil components after 130 days (Canul-Chan et al 2017). The presence of these substrates changed the collective metabolic functionality and affected the population structure generating a great genera divergence.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of a phenol-adapted microbial community: degradation capacity, taxonomy and metabolic description

et al. 2019

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Aims In this study, the ability of the consortium MR‐01 to degrade phenol was determined. The effects of this chemical on the taxonomy and the metabolic behaviour were analysed through metagenomics. Methods and Results Consortium MR‐01 was acclimated in a sublethal concentration of phenol. After this process, the capacity to degrade this molecule was analysed. Results showed that degradation increased with the increment of the initial phenol concentration. Metagenomic analysis indicates that the consortium metabolized phenol under aerobic conditions using phenol 2‐monooxygenase and the meta‐cleavage pathway. Sequence of the enzymes involved in the phenol degradation was ascribed to the Actinomycetales and Chloroflexales orders, with relative abundances <1%. The most abundant genera were part of the Sphingomonadales order; however, the role of these species in the consortium is not clear. Conclusions Consortium MR‐01 degrades efficiently high concentrations of phenol. The participation of extremophiles in the degradation process and the emergence of beneficial metabolic dependencies between the community members are some of the strategies used by the consortium to survive and develop under harsh environmental conditions. Significance and Impact of the Study This is one of the few studies describing the taxonomy and metabolic profile of a phenol degrading consortium.

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“…The biodegradation potential of crude petroleum has been assessed by hydrocarbonoclastic bacteria isolated from Soummam wadi sediments (Yalaoui-Guellal et al, 2020). The hexadecane uptake has been identified using an oil-degrader consortium (Hernández-Martínez et al, 2019;Sandoval-Herazo et al, 2020), and the evaluation of the ability to degrade crude and fuel oil has been determined by a native microbial consortium (NMC) (Canul-Chan et al, 2018;Rani et al, 2020). Besides, the enhancement of microorganism potential to degrade petroleum hydrocarbons can be improved using bioreactors, such as packed-bed bioreactors (PBBRs) operated in continuous mode (Kureel et al, 2018;Sonwani et al, 2020), fluidized bed bioreactors (Kuyukina et al, 2017), inverse fluidized bed bioreactors (Mallikarjuna and Dash, 2020), and a PBBR (Hussain et al, 2015;Kumar et al, 2015;Berrelleza-Valdez et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Motor oil wastewater treatment in a packed bed bioreactor using immobilized native microbial consortium

Houbron¹,

Cruz-Carmona²,

Ponciano-Rosas³

et al. 2021

RMIQ

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Population structures shift during the biodegradation of crude and fuel oil by an indigenous consortium

Cited by 25 publications

References 28 publications

Dispersant Enhances Hydrocarbon Degradation and Alters the Structure of Metabolically Active Microbial Communities in Shallow Seawater From the Northeastern Gulf of Mexico

Dispersant Enhances Hydrocarbon Degradation and Alters the Structure of Metabolically Active Microbial Communities in Shallow Seawater From the Northeastern Gulf of Mexico

Analysis of a phenol-adapted microbial community: degradation capacity, taxonomy and metabolic description

Motor oil wastewater treatment in a packed bed bioreactor using immobilized native microbial consortium

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