Population structure of the glasshouse whitefly <scp><i>Trialeurodes vaporariorum</i></scp> (Hemiptera: Aleyrodidae) shows multiple introductions to the U.K.

Kareem, Ali A.; Port, Gordon; Wolff, Kirsten

doi:10.1111/afe.12314

Cited by 7 publications

(12 citation statements)

References 61 publications

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“…PCR was performed using species‐specific markers for the 16S rRNA genes in Portiera, Wolbachia , Rickettsia, Hamiltonella , and Cardinium and the 23S rRNA genes in Arsenophonus and Fritschea (Table S2) (Kapantaidaki et al, ). The protocol for PCR amplification was as described above for the mtCO1 sequencing (Kareem et al, ). To check the quality of the DNA samples that showed no PCR products for symbiotic bacteria, we tested for the primary endosymbiont P. aleyrodidarum using primers for the 16S rRNA gene (518F and 799R Chelius & Triplett, ).…”

Section: Methodsmentioning

confidence: 99%

“…Genomic DNA (gDNA) from adult whitefly was extracted, as described in Tsagkarakou, Tsigenopoulos, Gorman, Lagnel, and Bedford (2007) (see also Kareem, Port, & Wolff, 2019). Mitochondrial cytochrome oxidase subunit one (mtCOI) of three individuals of B. tabaci from each population was sequenced (a total of 42 sequences).…”

Section: Mtcoi Sequencingmentioning

confidence: 99%

“…A polymerase chain reaction (PCR) was performed using primers: C1-J-2195 (5' TTGATT TTT TGGTCATCC AGA AGT 3' (Frohlich, Torres-Jerez, Bedford, Markham, & Brown, 1999) and tRNA-1576 (5' TAT AAA TCT TAA ATTTAC TGC A 3' (Tsagkarakou et al, 2007). PCR reactions, PCR product purification, DNA sequencing, sequencing product purification and visualization were performed as described in Kareem et al(2019). The sequences were checked using Geneious, version 6.1.4 (Kearse et al, 2012) and compared with those available in GenBank using the BLAST algorithm of the National Center for Biotechnology Information (NCBI).…”

Section: Mtcoi Sequencingmentioning

confidence: 99%

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Bemisia tabaci in Iraq: Population structure, endosymbiont diversity and putative species

Kareem

Logan

Port

et al. 2020

J Applied Entomology

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Whiteflies (Hemiptera: Aleyrodidae) are major pests of many crops worldwide. Bemisia tabaci is a cryptic species complex composed of more than 39 putative species. Understanding which putative species of B. tabaci are predominant in an area is vital for effective pest management since they may vary considerably with respect to insecticide resistance, host plant range and virus transmission. Here, for the first time, the genetic diversity, the symbiont diversity and population structure of B. tabaci in Iraq were studied. Fourteen populations were analysed using mitochondrial cytochrome C oxidase subunit 1 (mtCO1) sequencing and microsatellite genotyping. Symbiotic bacteria were identified using 16S rRNA and 23S rRNA sequencing. MtCO1 sequencing detected two putative species of B. tabaci. The predominant putative species in Iraq was Middle East‐Asia Minor (MEAM) 1 subcladeB2. In addition, one individual was MEAM1‐subcladeB. The second putative species was a single individual of MEAM2. The microsatellite data indicated low genetic diversity, with no biologically informative clustering. All MEAM1 individuals harboured one primary symbiont, Portiera aleyrodidarum, and most (96%) have two secondary symbionts: Hamiltonella sp. and Rickettsia sp. This study has identified the genetic diversity and population structure of B. tabaci in Iraq. Further investigation is needed to update the pest status of B. tabaci in this region. The current data, combined with investigations into the capacity of the various putative species to transmit plant viruses, especially tomato yellow leaf curl virus, will aid pest management and horticultural production.

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Section: Methodsmentioning

confidence: 99%

Section: Mtcoi Sequencingmentioning

confidence: 99%

Section: Mtcoi Sequencingmentioning

confidence: 99%

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Bemisia tabaci in Iraq: Population structure, endosymbiont diversity and putative species

Kareem

Logan

Port

et al. 2020

J Applied Entomology

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“…USA). Sequencing products were purified using ethanol precipitation following Kareem et al (2018) and visualised on an ABI3130XL Gene Analyser.…”

Section: Amplification and Sanger Sequencing Of Mtdnamentioning

confidence: 99%

Population structure, gene flow and relatedness of Natterer’s bats in Northern England

Mordue

Aegerter²,

Mill

et al. 2021

Mamm Biol

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There have been significant declines in population numbers of many bat species in the United Kingdom, including Natterer’s bats Myotis nattereri, over the last century, largely due to anthropogenic changes. The philopatry, which temperate-zone bats often exhibit to their natal landscapes, in combination with anthropogenic threats, can lead to fragmentation, isolation and sub-division of populations. This may result in bottlenecks and declines in genetic diversity. Multi-scaled research is required to disentangle how the variation in the physical traits of bat species (e.g. affecting flight), as well as their social and behavioural traits (e.g. community size, migration, breeding systems), may affect the genetic health of populations and provide a potential buffer against fragmentation. We used microsatellite markers to characterise the genetic diversity and population structure present in Natterer’s bat colonies to determine whether summer roosting bat colonies were spatially differentiated or part of a meta-population. Analyses of population structure and measures of genetic relatedness suggest spatially differentiated populations of bats exhibit long term site fidelity to summer roosting sites, whilst high genetic diversity at sites indicates gene exchange occurs via swarming sites. Natterer’s bats in northern England may travel greater distances to swarming sites than has been previously documented.

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“…As a result, the intensity of photosynthesis decreases, the content of chlorophyll changes the colour of the leaves (Park andLee, 2002& Abdellseid et al, 2014). The infested cell can be more damaging to plants when infested with insects pests at the same time such as whitefly and aphids (Kareem et al, 2019). Currently, a chemical method is considered the most effective for controlling the spider mite T. urticae, especially abamectin a microbial derivative-based acaraicide and other synthetic insecticides such as organophosphorous and pyrethroids in Egypt.…”

Section: Introductionmentioning

confidence: 99%

Efficacy of Certain Insecticides for Controlling the Two Spotted Spider Mite, Tetranychus urticae (Koch) and Determination Residues of Abamectin on Some Quality Parameters of Cucumber Fruits

Salem

2020

Egyptian Academic Journal of Biological Sciences, F. Toxicology

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Field trials were conducted in El-Salhia, Sharkia governorate, to assess the effectiveness of insecticides (imidaclopride, lambdacyhalothrin, and abamectin) against the two-spotted spider mite, Tetranychus urticae, on cucumber crops. The trial used a randomised complete block design (RCBD) with three treatments: T1: abamectin, T2: lambda-cyhalothrin, T3: imidaclopride, and control, which was repeated three times. At varied harvest intervals of (2h), 1, 3, 5, 7, 9-, 11-, 13-, and 15days following insecticide applications, residues and abamectin dissipation were measured. The insecticide's persistence, dissipation, half-life value, and safe harvest interval in cucumber were estimated. Additionally, the effects of abamectin residues on various quality indices (total soluble sugars, glucose, acidity, total soluble solids, ascorbic acid, -carotene, dry matter, and protein) were assessed in the last three samples (7 and 15 days after applications). The results revealed that all the treatments reduced the two-spotted spider populations. Furthermore, the results showed that the treatments sprayed with abamectin gave the highest reduction percentage (96.32%), followed by lambda-cyhalothrin (92.22%) and imidaclopride (76.93%). The halflife (t12) values in cucumber fruits were 2.99 days, and the loss percentages of initial deposits in cucumber fruits were 8.96 mg/ kg. In conclusion, we encourage farmers to use abamectin according to the Ministry of Agriculture recommendation in combating T. urticae, in integrated pest management, as cucumbers can be safely consumed after 7 days of abamectin treatment.

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Population structure of the glasshouse whitefly Trialeurodes vaporariorum (Hemiptera: Aleyrodidae) shows multiple introductions to the U.K.

Cited by 7 publications

References 61 publications

Bemisia tabaci in Iraq: Population structure, endosymbiont diversity and putative species

Bemisia tabaci in Iraq: Population structure, endosymbiont diversity and putative species

Population structure, gene flow and relatedness of Natterer’s bats in Northern England

Efficacy of Certain Insecticides for Controlling the Two Spotted Spider Mite, Tetranychus urticae (Koch) and Determination Residues of Abamectin on Some Quality Parameters of Cucumber Fruits

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