Population structure of multidrug-resistant Mycobacterium tuberculosis clinical isolates in Colombia

Rodríguez-Castillo, Juan Germán; Llerena, Claudia; Argoty-Chamorro, Lorena; Guerra, Julio; Couvin, David; Rastogi, Nalin

doi:10.1016/j.tube.2020.102011

Cited by 5 publications

(12 citation statements)

References 29 publications

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“…Additionally, information from MTBC strains from Ecuador and Colombia was retrieved from the bibliography, as it has been done in similar studies ( 27 , 28 ). For the phylogenetic analysis described below, 190 MTBC strains from Colombia for the years 2012–2014 ( 4 ) and 385 MTBC strains from Ecuador from the years 2012–2016 ( 9 , 26 ) were included in the study.…”

Section: Methodsmentioning

confidence: 99%

“…However, total notified TB cases in both countries are lower than the estimates provided by the WHO, with 5,595 cases notified in Ecuador and 13,659 cases in Colombia, suggesting an underestimation of TB cases in both countries ( 8 , 13 , 14 ). The most predominant MTBC lineage in Colombia is lineage 4 (including sublineages like LAM, Haarlem, X, and S) but also lineage 2–sublineage Beijing ( 4 , 15 ). Colombia and Peru have a significantly higher presence of MTBC lineage 2–sublineage Beijing than other countries in South America, representing a potential risk for TB control in the region ( 4 , 15–22 ).…”

Section: Introductionmentioning

confidence: 99%

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A first insight into tuberculosis transmission at the border of Ecuador and Colombia: a retrospective study of the population structure of Mycobacterium tuberculosis in Esmeraldas province

Castro-Rodriguez,

Espinoza-Andrade,

Franco-Sotomayor

et al. 2024

Front. Public Health

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ObjectiveTuberculosis (TB) is a major public health concern in Ecuador and Colombia, considering that both countries are high-burden TB settings. Molecular epidemiology is crucial to understand the transmission dynamics of Mycobacterium tuberculosis complex (MTBC) and to identify active transmission clusters of regional importance.MethodsWe studied the potential transmission of TB between Colombia and Ecuador through the analysis of the population structure of MTBC lineages circulating in the Ecuadorian province of Esmeraldas at the border with Colombia. A total of 105 MTBC strains were characterized by 24-loci MIRU-VNTR and spoligotyping.ResultsMTBC lineage 4 is only present in Esmeraldas; no MTBC strains belonging to Lineage 2–sublineage Beijing were found despite its presence in other provinces of Ecuador and, in Colombia. Genotyping results revealed a high degree of diversity for MTBC in Esmeraldas: Neither active transmission clusters within this province nor including MTBC strains from Colombia or other provinces of Ecuador were found.ConclusionOur data suggest that tuberculosis dynamics in this rural and isolated area may be not related to highly transmitted strains but could be influenced by other health determinants that favor TB relapse such as poverty and poor health system access. Further studies including a larger number of MTBC strains from Esmeraldas are necessary to test this hypothesis.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A first insight into tuberculosis transmission at the border of Ecuador and Colombia: a retrospective study of the population structure of Mycobacterium tuberculosis in Esmeraldas province

Castro-Rodriguez,

Espinoza-Andrade,

Franco-Sotomayor

et al. 2024

Front. Public Health

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“…Contrary to these reports, in Buenaventura, a region of Valle del Cauca, the Beijing lineage, clearly associated with resistant cases has been identified [ 33 ]. Most recent reports on TB-MDR Colombian isolates using spoligotyping showed unique TB-MDR strains with no support of active circulation of MDR clones and no relation to specific lineage (Beijing vs. LAM) [ 36 ]. Moreover, there is an extensive circulation (53%) of orphan lineages between the indigenous population [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

“…Most recent reports on TB-MDR Colombian isolates using spoligotyping showed unique TB-MDR strains with no support of active circulation of MDR clones and no relation to specific lineage (Beijing vs. LAM) [ 36 ]. Moreover, there is an extensive circulation (53%) of orphan lineages between the indigenous population [ 36 ]. Both studies emphasize the importance of using WGS as the primary method for phylogenetic analysis due to high resolution and discriminatory power and potentially characterize new lineages and outbreaks [ 37 , 38 ].…”

Section: Discussionmentioning

confidence: 99%

Phylogenomic analysis and Mycobacterium tuberculosis antibiotic resistance prediction by whole-genome sequencing from clinical isolates of Caldas, Colombia

et al. 2021

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Mycobacterium tuberculosis (M. tuberculosis) was the pathogen responsible for the highest number of deaths from infectious diseases in the world, before the arrival of the COVID-19 pandemic. Whole genome sequencing (WGS) has contributed to the understanding of genetic diversity, the mechanisms involved in drug resistance and the transmission dynamics of this pathogen. The object of this study is to use WGS for the epidemiological and molecular characterization of M. tuberculosis clinical strains from Chinchiná, Caldas, a small town in Colombia with a high incidence of TB. Sputum samples were obtained during the first semester of 2020 from six patients and cultured in solid Löwenstein-Jensen medium. DNA extraction was obtained from positive culture samples and WGS was performed with the Illumina HiSeq 2500 platform for subsequent bioinformatic analysis. M. tuberculosis isolates were typified as Euro-American lineage 4 with a predominance of the Harlem and LAM sublineages. All samples were proven sensitive to antituberculosis drugs by genomic analysis, although no phenotype antimicrobial tests were performed on the samples, unreported mutations were identified that could require further analysis. The present study provides preliminary data for the construction of a genomic database line and the follow-up of lineages in this region.

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“…No obstante, la incidencia de tuberculosis no solo aumenta por las condiciones sociales. En la actualidad, el control inmunológico de la primoinfección está siendo puesto a prueba por situaciones como el incremento del número de pacientes positivos para HIV, el uso de esteroides como parte del tratamiento para la infección por SARS-Cov-2, la multirresistencia de las micobacterias y la administración de medicamentos inmunosupresores en pacientes con trasplantes o para el tratamiento de enfermedades reumatológicas y neoplásicas (5)(6)(7)(8). Por ello, se necesitan aproximaciones diagnósticas que nos permitan la demostración rápida y precisa de la micobacteria mediante técnicas de laboratorio complementarias.…”

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Coloración de Ziehl-Neelsen en el laboratorio de patología: rendimiento y contribución al diagnóstico de micobacterias en el lavado broncoalveolar

2022

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Introducción. La coloración de Ziehl-Neelsen, con más de 100 años de uso, continúa vigente mundialmente.Objetivo. Comparar el rendimiento de las pruebas diagnósticas utilizadas para la determinación de micobacterias en el laboratorio clínico de patología en muestras de lavado broncoalveolar.Materiales y métodos. Se revisaron retrospectivamente 737 muestras de lavado broncoalveolar procesadas en el 2019 y el 2020 en el Hospital San Vicente Fundación (Medellín, Colombia) y se compararon las características de tres pruebas diagnósticas realizadas en paralelo: la reacción en cadena de la polimerasa (PCR) para micobacterias con detección de resistencia, el cultivo, y la coloración de Ziehl-Neelsen.Resultados. Se catalogaron como enfermos a 93 de los 737 pacientes a partir de los resultados positivos en alguna de las tres pruebas. El cultivo tuvo una sensibilidad de 0,80, la PCR una de 0,76 y la coloración de Ziehl-Neelsen una de 0,51. Sin embargo, solo 5 de 75 (6,5 %) cultivos fueron positivos a las cuatro semanas y el resto lo fue a las ocho semanas. La PCR combinada con la coloración de Ziehl-Neelsen mejoró la sensibilidad de la PCR por sí sola, de 0,76 a 0,88, diferencia que fue estadísticamente significativa (p=0,022).Conclusión. En las muestras de lavado broncoalveolar, el cultivo sigue siendo la prueba con mejor sensibilidad. El uso conjunto de la prueba de PCR y la coloración de Ziehl-Neelsen mejora significativamente la sensibilidad de la primera, lo que compensa la demora relativa en la entrega de los resultados debida al tiempo requerido para la tinción de Ziehl-Neelsen.

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Population structure of multidrug-resistant Mycobacterium tuberculosis clinical isolates in Colombia

Cited by 5 publications

References 29 publications

A first insight into tuberculosis transmission at the border of Ecuador and Colombia: a retrospective study of the population structure of Mycobacterium tuberculosis in Esmeraldas province

A first insight into tuberculosis transmission at the border of Ecuador and Colombia: a retrospective study of the population structure of Mycobacterium tuberculosis in Esmeraldas province

Phylogenomic analysis and Mycobacterium tuberculosis antibiotic resistance prediction by whole-genome sequencing from clinical isolates of Caldas, Colombia

Coloración de Ziehl-Neelsen en el laboratorio de patología: rendimiento y contribución al diagnóstico de micobacterias en el lavado broncoalveolar

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