Population Structure ofCandida albicansfrom Three Teaching Hospitals in Ghana

Abstract

Previous studies on Candida species in a clinical setting in Ghana have shown a prevalence of Candida albicans. Despite this, very little is known about the various strain types and their population genetic structure. In this study three microsatellite loci, CAI, CAIII and CAVI, were used to investigate the population genetic structure of C. albicans from clinical isolates in Ghana. In all, 240 clinically unrelated C. albicans isolates were recovered from patients reporting at three teaching hospitals. All the isolates were heterozygous for at least one of the three loci, except for one isolate, which was homozygous for all three loci. Sixty-seven unique alleles and 240 different genotypes were generated by the three polymorphic microsatellite loci, resulting in a very high discriminatory potential of approximately 0.98. There was no significant difference in allele frequencies from the small number of anatomical sites sampled, regardless of the host conditions although high genotypic diversities were observed among the isolates. There was evidence for clonal reproduction, including over-expression of observed heterozygotes across the populations. The populations deviated significantly from Hardy-Weinberg equilibrium and pair-wise genotypic linkage disequilibria comparisons across the three loci were significant, also suggesting a clonal population. The overall Wright FIS for the three loci was negative, and the overall FST value was not significantly different from zero for the three loci analyzed, indicating a clonal and homogeneous population across the three sampling locations from Ghana.

Genotypic diversity and antifungal susceptibility pattern of Candida albicans species isolated from hospitalized paediatric patients with urinary tract infection in Iran

“…This may be attributable to the migration of the host population between wards. In this connection, Adjapong et al (2016) suggested that the high diversity and low structure within a population may be explained by sexual reproduction.…”

“…In this connection, Adjapong et al . (2016) suggested that the high diversity and low structure within a population may be explained by sexual reproduction.…”

Genotyping and Drug Resistance Profile of Clinical Isolates of Candida albicans from Vulvovaginal Candidiasis in the Eastern China

“…In addition, it is important to look into the population genetic structure and epidemiology of C. albicans infections because of increasing reports indicating dynamic changes in the antifungal susceptibility profile with different genotypes and geographic origins [ 6 ]. Thus, understanding the genetic diversity of C. albicans could help clinicians to implement appropriate diagnostic, therapeutic and preventive strategies [ 7 , 8 ]. Microsatellite analysis is highly reproducible with strong discriminatory power and is widely applied in molecular typing of fungal pathogens [ 9 ].…”

Genotyping and Drug Resistance Profile of Clinical Isolates of Candida Albicans From Vulvovaginal Candidiasis in The Eastern China

“…In addition, it is important to look into the population genetic characteristics of C. albicans infections owing to increasing reports indicating dynamic antifungal susceptibility pro le with different genotype and geographic origin [6]. Thus, understanding the genetic diversity of C. albicans could help clinicians to implement appropriate diagnostic, therapeutic and preventive strategies [7,8]. Microsatellite marker is highly reproducible with strong discriminatory potential and is widely applied in molecular typing [9].…”