Population structure in the Andaman keelback,Xenochrophis tytleri: geographical distance and oceanic barriers to dispersal influence genetic divergence on the Andaman archipelago

Abstract

Limited gene flow between populations due to geographic distance, presence of barriers or inherent low dispersal ability leads to the formation of genetically structured populations. Strong population structure indicates lowered levels or absence of gene flow which might lead to inbreeding and loss of genetic capacity to recuperate from anthropogenic stress and natural calamities. Terrestrial reptiles are generally known to have low dispersal abilities and few studies have explored drivers of their population structure on continental islands, where both anthropogenic stress and natural calamities are relatively common. We investigated the population structure and drivers of diversification of the Andaman keelback (Xenochrophis tytleri), an endemic, terrestrial and freshwater snake species in the Andaman archipelago, a continental group of islands in the Bay of Bengal. Data was collected from 86 individuals from seven islands and 78 individuals were sequenced for the gene Nuclear Dehydrogenase subunit 4 to identify the number of populations and distribution of genetic diversity across populations. We found 11 haplotypes on seven islands and observed high genetic differentiation between seven populations defined island-wise (FST = 0.82). We further tested the number of populations by incorporating spatial data into Bayesian Clustering Analysis (GENELAND) and identified six populations of the Andaman keelback. We tested for the influence of Isolation-by-distance on these populations. While the overall trend showed a positive correlation between geographic and genetic distance, a correlogram revealed that the positive correlation disappears beyond ∼20–40 km. We also tested for the presence of geographical barriers to gene flow using Monmonier’s algorithm (SPADS), which identified five barriers to dispersal confirming that there are oceanic barriers to dispersal for some island populations of the Andaman keelback. As the Andaman Islands are arranged almost in a straight line from North to South, our data are insufficient to tease apart the roles of geographical distance and barriers to gene flow. We conclude that salt waters between near islands are weak barriers and as the geographical distance between islands increases, so does the strength of the barrier.

The Andaman day gecko paradox: an ancient endemic without pronounced phylogeographic structure

“…5), even in the Greater Andaman Islands. A study on the Andaman Keelback (Fowlea tytleri) also detected structured populations through mitochondrial phylogeography 13 . However, all four species, including the Andaman Keelback, show unique haplotypes on Little Andaman, reflecting more restricted gene flow between Little Andaman and other islands (Fig.…”

“…There are just a handful of studies attempting to understand the effects of relatively recent island separation on populations of endemic terrestrial fauna in the Andaman Islands. Phylogeographic studies on four bat species 12 and the Andaman keelback snake (Fowlea tytleri (Blyth, 1863)) 13 showed substantial levels of genetic diversity, structure and varying degrees of inter-island population differentiation in mitochondrial DNA.…”