Abstract:Laboratory rabbits used in Taiwan are primarily supplied by the Livestock Research Institute (LRI) and the Animal Drugs Inspection Branch (ADIB) of the Animal Health Research Institute. An analysis of the genetic characteristics and structure of these populations would thus be a fundamental step in building a long-term management programme for maintaining stable animal quality and preserving the genetic variation among the populations. In this study, DNA samples were isolated from founders of 5 populations: Ne… Show more
“…Moreover, results in Table S2 showed that 57 % of loci were highly formative in terms of PIC values. The high percentage of formative PIC was also observed in several studies (Alves et al, 2015;El-Aksher et al, 2016;Lai et al, 2018). On the other hand, 89 % of the loci were significant in terms of HWE at three significance levels (P <0.05, P <0.01, and P <0.001), which is generally characteristic for an inbreeding situation in NARs.…”
Abstract. Having higher adaptability against abiotic stress, which
is characterized in rural areas in developing countries, local farm animal
genetic resources (FAGRs) are increasingly precarious for random and
unsystematic crossing with exotic breeds. In this study, 85
microsatellite loci were utilized to assess genetic diversity among native
Algerian rabbits (NARs) sampled from an area of 753 km (from north
to south) and 919 km (from east to west). Those distances covered
25 significant geographical points in seven rural areas (El Taref, Mostaganem,
Sidi Bel Abbès, M'Sila, Dar Chioukh, Faidh El Botma, and Laghouat). A
total of 558 alleles were observed in this study. The highest genetic
diversity was registered in the southern direction among NAR populations. The
mean number of alleles per locus (MNa) and the inbreeding coefficient (FIS)
were highest in Laghouat (4.482 and 0.232), while they were lowest in El Taref
(4.000 and 0.149). In the current study, the number of private alleles (Pa)
ranged from 9 to 23. In addition, the average of observed heterozygosity
(0.427) was lower than the expected value (0.524) due to high levels of
inbreeding. The discriminant analysis of principal components
(DAPC), the neighbor-joining tree (NJ), and the analysis of STRUCTURE software confirmed the
classification of populations according to geographical zones into four
main groups (east, west, south, and middle). The results of the current
study are useful for breeding improvement and conservation plan research in relation to
local animal genetic resources in Algeria.
“…Moreover, results in Table S2 showed that 57 % of loci were highly formative in terms of PIC values. The high percentage of formative PIC was also observed in several studies (Alves et al, 2015;El-Aksher et al, 2016;Lai et al, 2018). On the other hand, 89 % of the loci were significant in terms of HWE at three significance levels (P <0.05, P <0.01, and P <0.001), which is generally characteristic for an inbreeding situation in NARs.…”
Abstract. Having higher adaptability against abiotic stress, which
is characterized in rural areas in developing countries, local farm animal
genetic resources (FAGRs) are increasingly precarious for random and
unsystematic crossing with exotic breeds. In this study, 85
microsatellite loci were utilized to assess genetic diversity among native
Algerian rabbits (NARs) sampled from an area of 753 km (from north
to south) and 919 km (from east to west). Those distances covered
25 significant geographical points in seven rural areas (El Taref, Mostaganem,
Sidi Bel Abbès, M'Sila, Dar Chioukh, Faidh El Botma, and Laghouat). A
total of 558 alleles were observed in this study. The highest genetic
diversity was registered in the southern direction among NAR populations. The
mean number of alleles per locus (MNa) and the inbreeding coefficient (FIS)
were highest in Laghouat (4.482 and 0.232), while they were lowest in El Taref
(4.000 and 0.149). In the current study, the number of private alleles (Pa)
ranged from 9 to 23. In addition, the average of observed heterozygosity
(0.427) was lower than the expected value (0.524) due to high levels of
inbreeding. The discriminant analysis of principal components
(DAPC), the neighbor-joining tree (NJ), and the analysis of STRUCTURE software confirmed the
classification of populations according to geographical zones into four
main groups (east, west, south, and middle). The results of the current
study are useful for breeding improvement and conservation plan research in relation to
local animal genetic resources in Algeria.
“…Moreover, the genetic information provided by morphological and biochemical markers is limited; hence, they are not used to identify single base mutations. Owing to the difficultly in comprehensively evaluation the genetic background of laboratory animal, molecular marker technology have been introduced into the study of genetics, such as SSRs, which can provide sufficient genetic information, and have become available for detection of genetic diversity of laboratory animals [5]. However, as a third-generation DNA molecular marker technology and an excellent tool for population based genetic research [6,7], the research of using single nucleotide polymorphisms (SNPs) in genetic variability evaluation of closed colony is still inadequate.…”
The complex genetic architecture of closed colonies during successive passages poses a significant challenge in the understanding of the genetic background. Research on the dynamic changes in genetic structure for the establishment of a new closed colony is limited. In this study, we developed 51 single nucleotide polymorphism (SNP) markers for the rare minnow (Gobiocypris rarus) and conducted genetic diversity and structure analyses in five successive generations of a closed colony using 20 SNPs. The range of mean Ho and He in five generations was 0.4547–0.4983 and 0.4445–0.4644, respectively. No significant differences were observed in the Ne, Ho, and He (p > 0.05) between the five closed colony generations, indicating well-maintained heterozygosity. The F-statistics analysis revealed a relatively stable genetic structure of the closed colony. Furthermore, the genetic distance between the newer and older generations increased with the breeding generations in closed colonies. Our results confirmed previous findings in the same samples using microsatellite markers. The results will be beneficial for establishing genetic variability monitoring criteria and restoration of the wild population of the rare minnow and other laboratory fish.
<p>The limited rabbit resources in Egypt are threatened by the danger of extinction, whereas genetic diversity studies of native breeds could play a vital role in conservation and improvement of these breeds. In this study, 3 native rabbit breeds: Gabali (G), Baladi Red (BR) and Baladi Black (BB), in addition to New Zealand White (NZW), were genotyped using 12 microsatellite markers. All the typed microsatellites were polymorphic by average number of alleles 5.25 per locus. Observed and expected heterozygosity per locus averaged 0.62 and 0.68, respectively. The average polymorphic information content was 0.71 and the highest polymorphic information content was recorded in locus SOL33 by 0.85. All the studied loci except SAT7 and SAT2 showed deviation from Hardy-Weinberg equilibrium with significant level. The inbreeding coefficient of the individuals relative to the total population was 0.07. The within-population heterozygote deficit averaged 0.07 and ranged from 0.141 in BR to 0.015 in BB breeds. The highest pairwise differentiation among the populations was recorded between BB and NZW (0.071), while the lowest value was recorded between BR and both of G (0.038) and BB (0.039). The lowest pairwise Nei’s genetic distance was recorded between BR and BB (0.190), while the highest was recorded between NZW and BB breeds (0.409). BR and G populations were clustered together forming an admixed mosaic cluster. BR recorded the highest contribution in the aggregate genetic diversity based on the three prioritisation methods used.</p>
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