Population structure and genetic analysis of narrow-clawed crayfish (Astacus leptodactylus) populations in Turkey

Abstract

The genetic differentiation among Turkish populations of the narrow-clawed crayfish was investigated using a partial sequence of cytochrome oxidase subunit I gene (585 bp) of 183 specimens from 17 different crayfish populations. Median joining network and all phylogenetic analyses disclosed a strong haplotype structure with three prominent clades diverged by a range between 20 and 50 mutations and substantial inter-group pairwise sequence divergence (5.19-6.95 %), suggesting the presence of three distinct clades within the Anatolian populations of Astacus leptodactylus. The divergence times among the three clades of Turkish A. leptodactylus are estimated to be 4.96-3.70 Mya using a molecular clock of 1.4 % sequence divergence per million years, pointing to a lower Pliocene separation. The high level of genetic variability (H d = 95.8 %, π = 4.17 %) and numerous private haplotypes suggest the presence of refugial populations in Anatolia unaffected by Pleistocene habitat restrictions. The pattern of genetic variation among Turkish A. leptodactylus populations, therefore, suggests that the unrevealed intraspecific genetic structure is independent of geographic tendency and congruent with the previously reported geographic distribution and number of subspecies (A. l. leptodactylus and A. l. salinus) of A. leptodactylus.

Monitoring of noble, signal and narrow-clawed crayfish using environmental DNA from freshwater samples

“…To minimize erroneous identification of crayfish, validated identification keys that abstain from using colour patterns should preferably be used [27] coupled with analysis of genetic markers. A cube of approximately 0.5 cm 2 of tail muscles were collected from each specimen for sequence analysis, allowing us to key nucleotide sequence variation with morphological variation and match with the already known phylogenetic structures of European crayfish [29, 30]. For A .…”

Kerevitlerde Genetik Şifre Belirlemenin Önemi ve Kullanılan Yöntemler

“…Furthermore, researchers have found the highest genetic diversity in the Black Sea basin and especially in the Western Balkans and other Black Sea populations. And also, Akhan et al (2014) investigated the genetic differentiation among Turkish populations of the narrow-clawed crayfish using a partial sequence of cytochrome oxidase subunit I gene (585 bp) of 183 specimens from 17 different crayfish populations. From this study they disclosed a strong haplotype structure with three prominent clades diverged by a range between 20 and 50 mutations and substantial inter-group pairwise sequence divergence (5.19-6.95 %), They determined the high level of genetic variability (Hd = 95.8 %, p = 4.17 %) and numerous private haplotypes.…”

Untitled

“…Besides protein-based methods, more reliable DNAbased methods are also being used (Agerberg, 1990;Fevolden et al, 1994). Single nucleotide polymorphism (SNP) (Akhan et al, 2014), amplified fragment length polymorphism (AFLP) (Chiesa et al, 2011), restricted fragment length polymorphism (RFLP) Grandjean et al, 1997;Gouin et al, 2003) and single sequence repeat (SSR) Yue et al, 2010;Gouin et al, 2011;Iorgu et al, 2011, Vorburger et al, 2014Ming et al, 2014) are some of the very useful methods that have been used for analyzing both population genetics and systematic classification of crayfish. These DNA-based methods provide rapid and accurate results that facilitate species identification and distinction between closely related species and subspecies of fish, crayfish and other organisms.…”

“…The taxonomists discover nearly 10 new species every year (Crandall and Buhay, 2008). Crayfish are represented by six indigenous and five nonindigenous species in Europe and by two species (Astacus leptodactylus and Austropotamobius torrentium) in Turkey (Harlıoğlu and Güner, 2006;Akhan et al, 2014).…”

“…Among these species, A. leptodactylus distributes in most of Turkish freshwater; while Austropotamobius torrentium distributes only in Trace region of Turkey. It is well known that crayfish have been transferred between water bodies due to anthropogenic effects (Akhan et al, 2014) such as introduction of crayfish into newly constructed dam lakes by fisherman or government fisheries officials. Monitoring of the crayfish species in water bodies is essential for their successful stock management and conservation of genetic diversity , which in turn requires fast methods of species identification.…”