Population structure and characterization of viridans group streptococci (VGS) including Streptococcus pneumoniae isolated from adult patients with cystic fibrosis (CF)

Maeda, Yasunori; Elborn, J. Stuart; Parkins, Michael D.; Reihill, James; Goldsmith, Colin E.; Coulter, Wilson A.; Mason, Charlene; Millar, B. Cherie; Dooley, James; Lowery, Colm; Ennis, Madeleine; Rendall, J.; Moore, John E.

doi:10.1016/j.jcf.2010.11.003

Cited by 52 publications

(44 citation statements)

References 21 publications

(17 reference statements)

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“…Oral commensal streptococci, including S. parasanguinis, have been shown to be prevalent in CF pulmonary infections (Maeda et al, 2011), which historically have been dominated by the presence of the major CF pathogen P. aeruginosa. Interestingly, the occurrence of oral commensal streptococci in the CF lung environment has been associated with improved lung function (Filkins et al, 2012); however, the mechanism by which S. parasanguinis benefits CF patients is unclear.…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, viridans streptococci, including Streptococcus parasanguinis, Streptococcus gordonii and Streptococcus sanguinis, have been detected in the sputum of cystic fibrosis (CF) patients (Maeda et al, 2011). Moreover, some oral commensal bacteria have been associated with improved lung function in patients with CF (Filkins et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Nitrite reductase is critical for Pseudomonas aeruginosa survival during co-infection with the oral commensal Streptococcus parasanguinis

Scoffield

2016

Microbiology

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Pseudomonas aeruginosa is the major aetiological agent of chronic pulmonary infections in cystic fibrosis (CF) patients. However, recent evidence suggests that the polymicrobial community of the CF lung may also harbour oral streptococci, and colonization by these micro-organisms may have a negative impact on P. aeruginosa within the CF lung. Our previous studies demonstrated that nitrite abundance plays an important role in P. aeruginosa survival during co-infection with oral streptococci. Nitrite reductase is a key enzyme involved in nitrite metabolism. Therefore, the objective of this study was to examine the role nitrite reductase (gene nirS) plays in P. aeruginosa survival during co-infection with an oral streptococcus, Streptococcus parasanguinis. Inactivation of nirS in both the chronic CF isolate FRD1 and acute wound isolate PAO1 reduced the survival rate of P. aeruginosa when co-cultured with S. parasanguinis. Growth of both mutants was restored when co-cultured with S. parasanguinis that was defective for H 2 O 2 production. Furthermore, the nitrite reductase mutant was unable to kill Drosophila melanogaster during co-infection with S. parasanguinis. Taken together, these results suggest that nitrite reductase plays an important role for survival of P. aeruginosa during co-infection with S. parasanguinis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Nitrite reductase is critical for Pseudomonas aeruginosa survival during co-infection with the oral commensal Streptococcus parasanguinis

Scoffield

2016

Microbiology

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“…The correlation observed between the fractional representation of Streptococcus and clinically stable disease (i.e., outpatients) prompted us to further characterize the streptococcal species in the patient samples. A selection of 13 samples (8 outpatients and 5 inpatients for which sufficient sputum-derived gDNA was available) was analyzed on the HOMIM, which contains probes for the detection of ϳ300 oral species, including the majority of Streptococcus species previously identified in CF patient sputum samples (17). Each analyzed sputum gDNA sample is scored based on relative intensity of hybridization for each probe on the microarray and is assigned a semiquantitative value of 0 to 5, where 0 is no hybridization above background and 5 is intense hybridization to probe.…”

Section: Fig 1 Characterization Of the Polymicrobial Communities Of Smentioning

confidence: 99%

“…Recent reports describe the prevalence of viridans streptococci in the analysis of patient sputum, including the salivarius, milleri (or anginosus), and mitis streptococcus groups (17). The Streptococcus milleri group (SMG), which includes S. anginosus, S. constellatus, and S. intermedius, may play a significant role in the pathogenesis of the CF lung (18,28) by influencing microbial community structures during periods of clinical stability or by causing acute exacerbation in diseased patients (27).…”

mentioning

confidence: 99%

Prevalence of Streptococci and Increased Polymicrobial Diversity Associated with Cystic Fibrosis Patient Stability

Filkins

Hampton

Gifford

et al. 2012

Journal of Bacteriology

171

200

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e Diverse microbial communities chronically colonize the lungs of cystic fibrosis patients. Pyrosequencing of amplicons for hypervariable regions in the 16S rRNA gene generated taxonomic profiles of bacterial communities for sputum genomic DNA samples from 22 patients during a state of clinical stability (outpatients) and 13 patients during acute exacerbation (inpatients). We employed quantitative PCR (qPCR) to confirm the detection of Pseudomonas aeruginosa and Streptococcus by the pyrosequencing data and human oral microbe identification microarray (HOMIM) analysis to determine the species of the streptococci identified by pyrosequencing. We show that outpatient sputum samples have significantly higher bacterial diversity than inpatients, but maintenance treatment with tobramycin did not impact overall diversity. Contrary to the current dogma in the field that Pseudomonas aeruginosa is the dominant organism in the majority of cystic fibrosis patients, Pseudomonas constituted the predominant genera in only half the patient samples analyzed and reported here. The increased fractional representation of Streptococcus in the outpatient cohort relative to the inpatient cohort was the strongest predictor of clinically stable lung disease. The most prevalent streptococci included species typically associated with the oral cavity (Streptococcus salivarius and Streptococcus parasanguis) and the Streptococcus milleri group species. These species of Streptococcus may play an important role in increasing the diversity of the cystic fibrosis lung environment and promoting patient stability.

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“…We decided to set this cut-off both for economic reasons (reduction of plate numbers) and because it is widely accepted for distinguishing potentially significant pathogens from colonisers 17 . another study that used selective mitis-salivarius agar with 1% tellurite to recover viridans group streptococci was able to recover smG in 8.9% (4/45) of adult cF patients, all isolates being S. anginosus 18 . Because smG have already been correlated with clinical data in cF patients with exacerbated disease and our data are in accordance with these findings, we did not analyse cases of smG positive cF patients further.…”

Section: Discussionmentioning

confidence: 98%

The Streptococcus milleri group in chronic obstructive pulmonary disease

Navrátilová¹,

Bardoň²,

Novotný³

et al. 2016

BIOMED PAP

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a,eBackground and Aims. S. anginosus, constellatus and intermedius, also known as the Streptococcus milleri group (SMG) are three streptococcal species more frequently detected in cases of invasive disease, abscesses and empyema in particular. Recent research suggests they play a role in exacerbations of cystic fibrosis (CF). Owing to poor recovery on standard culture media and difficult differentiation from non-pathogenic streptococci, SMG may be underdiagnosed in routine settings. We aimed to establish the incidence of SMG in chronic obstructive pulmonary disease (COPD) patients compared to CF patients and to examine possible links of SMG to exacerbations that plays a key role in progression of COPD. Methods. Altogether, 90 respiratory tract samples of patients suffering from CF or COPD were examined during the period from July 2012 to December 2013. Semi-selective McKay agar was used for primary cultivation of SMG and MALDI TOF MS was used for species identification that was confirmed by biochemical profiling and specific PCR. Results. We confirmed the presence of SMG in CF (17.6% incidence in adult patients) and newly established its presence in COPD (10.3% incidence). In COPD, SMG was detected in 4 cases of acute exacerbations, where no other bacterial pathogen was detected. In 3/4 cases, increased CRP level indicated bacterial infection as a cause of the exacerbation and in all 3 cases, patients recovered during antibiotic treatment. Conclusions. Our data indicate SMG may act as opportunist pathogens able to cause exacerbations in COPD.

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Population structure and characterization of viridans group streptococci (VGS) including Streptococcus pneumoniae isolated from adult patients with cystic fibrosis (CF)

Cited by 52 publications

References 21 publications

Nitrite reductase is critical for Pseudomonas aeruginosa survival during co-infection with the oral commensal Streptococcus parasanguinis

Nitrite reductase is critical for Pseudomonas aeruginosa survival during co-infection with the oral commensal Streptococcus parasanguinis

Prevalence of Streptococci and Increased Polymicrobial Diversity Associated with Cystic Fibrosis Patient Stability

The Streptococcus milleri group in chronic obstructive pulmonary disease

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