Population status and habitat modelling of high value vulnerable <i>Ferula jaeschkeana</i> for conservation in North-Western Himalaya

Paul, Shiv; Samant, S. S.; Lal, Madan; Sharma, Lipika; Ram, Jeet

doi:10.5958/0975-6892.2019.00017.0

Cited by 5 publications

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“…The plant is distributed across the Central Asia, Westward throughout the Mediterranean region to Northern Africa (Mabberley 2008). In India plant is reported from different part of Jammu and Kashmir, Himachal Pradesh and Uttarakhand (Smith and Krikorian 1988;Samant et al 2007;Sharma 2015;Dawa et al 2018;Paul et al 2019). Plant is one of the important medicinal herbs and used to cure different ailments in different parts of the Himalaya, roots and resin of the plant is used to cure rheumatism, toothache, wounds, poultices, tumors, chronic wounds and ulcers (Samant et al 1998;Khalilova and Saidkhodzhaev 1998;Samant et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Somatic embryogenesis and plant regeneration in Ferula jaeschkeana Vatke: a threatened medicinal herb

Sharma

Khajuria

2020

Vegetos

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An efficient in vitro propagation protocol for an important medicinal endangered herb Ferula jaeschkeana has been developed through somatic embryogenesis. 12 week old friable calli were derived from petiole explants on a medium supplemented with 2.0 mgl −1 2,4-D and 1.0 mgl −1 Kn and used as mother source for somatic embryogenesis. Different auxins (2,4-D, IBA, IAA and NAA) in different concentrations (0.2-4.0 mgl −1 ) was used to produce embryogenic callus. Eighty four percent calli produced globular embryos on a medium supplemented with 4.0 mgl −1 2,4-D. The globular embryo then mature on culture medium (Control) or supplemented with different addictives (auxin, proline and ABA), maturation was significantly affected by ABA, proline and number of subcultures with maximum number of mature embryo (32.1 ± 0.2) was formed on medium fortified with 2.0 mgl −1 proline. Mature embryos had two well-developed cotyledons and an elongated hypocotyl root axis. 78% of these embryos were transformed into plantlets when placed in culture medium fortified with 0.2 + 0.01 + 0.05 mgl −1 (BAP:IBA:GA 3 ) respectively. Regenerated plants were hardened in vermiculite with a 23% survival rate. The regenerated plants showed normal diploid chromosome number (2n = 22) and leaf morphology was comparable to in vivo plant.

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