Population Shifts in Heterotrophic Bacteria in a Tributary of the Saint John River as Measured by Taxometrics

Holder-Franklin, M. A.; Franklin, M.; Cashion, P.; Cormier, Chantale; Wuest, Lawrence

doi:10.1007/978-3-642-67034-3_10

Cited by 20 publications

(7 citation statements)

References 4 publications

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“…It is known that there can be diurnal and seasonal shifts in bacterial populations as a result of short and longer term changes in environn~ental conditions (Holder-Franklin et al 1978;Sneath et al 1981;Hollohan et al 1986 ). Temporal changes in species and biotypes can be expected and further investigation may reveal even greater diversity than shown in this study.…”

Section: Discussionmentioning

confidence: 98%

Numerical analysis and the application of random amplified polymorphic DNA polymerase chain reaction to the differentiation of Vibrio strains from a seasonally cold ocean

Martin-Kearley

Gow²,

Péloquin³

et al. 1994

Can. J. Microbiol.

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Eighty regional strains of Vibrio isolated from the seasonally cold waters of coastal Newfoundland, and a number of Vibrio reference cultures, were studied. The regional strains had been isolated from the brown macroalga Alaria esculenta and the giant scallop Placopecten magellanicus and were known to grow at 4 degrees C. The strains were grouped according to their arginine-dihydrolase reactions and examined by numerical analysis. According to phenotypic properties the arginine-dihydrolase positive strains closely resembled Vibrio splendidus biovar I. Most clusters of the arginine-dihydrolase negative strains appeared to be unique but the closest phenotypic resemblance among some strains was with Vibrio ordalii. Some strains were examined using the random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) technique for fingerprinting and it was shown that the regional strains were significantly different from either V. splendidus biovar I or V. ordalii. Generally, the strains from seaweed clustered separately from those that were from scallops. Strains in some clusters, especially those from the seaweed, were able to utilize most of the compounds that were tested as sole sources of carbon and energy.

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Section: Discussionmentioning

confidence: 98%

Numerical analysis and the application of random amplified polymorphic DNA polymerase chain reaction to the differentiation of Vibrio strains from a seasonally cold ocean

Martin-Kearley

Gow²,

Péloquin³

et al. 1994

Can. J. Microbiol.

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“…The use of multivariate analysis, however, did prove to be a fruitful approach to pursue this phenomenon. The advantages in microbial ecology of reducing the variability of a diverse population to its most salient factors is now becoming well documented (11,12). The formation of five distinct types of aquatic environments based on the factors of salinity, heterotrophic uptake, and particulate load was both fascinating and pertinent to this investigation.…”

Section: Discussionmentioning

confidence: 99%

Attached and free-floating bacteria in a diverse selection of water bodies

1982

Deep Sea Research Part B. Oceanographic Literature Review

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The contribution of attached and free-floating bacteria to the bacterial numbers and heterotrophic uptake in 44 diverse aquatic environments was studied. A factor analysis reduced the variability of the raw data base to three major factors explaining 53.6% of total variance. These factors were (i) salinity, (ii) heterotrophic uptake, and (iii) particulate load. A cluster analysis categorized the 44 habitats into five distinct environmental types based on these three factors. There was no significant pattern in the distribution of attached versus free-floating bacteria when assessed by epifluorescent microscopy. However the contribution of attached bacteria to the uptake of an amino acids mix was reduced in marine waters. Heavy particulate loads resulted in an increased percentage uptake of amino acids and glucose from the attached bacteria. Uptake response was found to be substrate specific especially in oliogotrophic freshwater. Amino acid uptake was more associated with the attached fraction, whereas glucose uptake was mediated more by the free-floating fraction.

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“…The taxonomic data were supported by genetic studies i.e., DNA analysis and DNA/DNA hybridization. (Holder-Franklin et al, 1978;Holder-Franklin et al, 1981;Holder-Franklin, 1981). Sites on the St. John River and several of its tributaries in New Brunswick were selected which were downstream from or located at areas with reduced dissolved oxygen levels.…”

Section: Population Studies Using Multivariate Statisticsmentioning

confidence: 99%

Aquatic microorganisms: processes, populations, and molecular solutions to environmental problems

Holder-Franklin

1992

J Aquat Ecosyst Stress Recov

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Studies on aquatic ecosystems at the trophic level of bacteria include population succession and the movement of species through the water column. Factor and path analysis of environmental parameters and the bacterial profiles indicate that the bacterial populations are under the control of environmental factors. The most important environmental factors in the Canadian study reported here are temperature followed by oxygen levels, nutrient levels, and ion concentrations. A major revolution in investigative approaches has begun in aquatic bacterial population studies using technology based on molecular methods. Finger print analysis of bacterial 16S RNA (molecular phylogeny) has not only changed the classification of bacteria but also the approach to solving environmental problems. The bacterial groups have been placed into species that are more functionally and ecologically aligned. Uncultured mixed biomass can be examined by gene probes for both procaryotes and eucaryotes to identify specific nucleotide sequences. Aquatic ecosystem health is maintained by the balanced biota and the process of biodegradation is an important stage in bioremediation. Control of toxic wastes in the waters and groundwaters can be accomplished by in situ bioremediation using indigenous microorganisms as demonstrated by the field study reported by Litchfield et al. (1990).

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Population Shifts in Heterotrophic Bacteria in a Tributary of the Saint John River as Measured by Taxometrics

Cited by 20 publications

References 4 publications

Numerical analysis and the application of random amplified polymorphic DNA polymerase chain reaction to the differentiation of Vibrio strains from a seasonally cold ocean

Numerical analysis and the application of random amplified polymorphic DNA polymerase chain reaction to the differentiation of Vibrio strains from a seasonally cold ocean

Attached and free-floating bacteria in a diverse selection of water bodies

Aquatic microorganisms: processes, populations, and molecular solutions to environmental problems

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