Population Research of Genetic Polymorphism at Amino Acid Position 631 in Chicken Mx Protein with Differential Antiviral Activity

Seyama, Takahito; Ko, Jae Hong; Ohe, Mika; Sasaoka, Noboru; Okada, Ayumi; Gomi, Hiroshi; Yoneda, Akihiro; Ueda, Junji; Nishibori, Masahide; Okamoto, Susumu; Maeda, Yoshizane; Watanabe, Tomomasa

doi:10.1007/s10528-006-9040-3

Cited by 39 publications

(42 citation statements)

References 25 publications

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“…Results have shown that mice carrying the MX1 gene with a large deletion or a nonsense mutation are influenza virus-susceptible, have decreased metabolic stability of MX1 mRNA, and lack the ability to synthesize the MX1 protein (Staeheli et al 1988). A single amino acid substitution between Asn and Ser at position 631 in the chicken MX1 protein has been reported to determine resistant and sensitive antiviral activity (Seyama et al 2006;Wang et al 2012). In our study, the g. 143196228 G>A mutation leads to a substitution of arginine to histidine at position 419 in predicted N-terminal GTPase domain, which might alter the MX1 function and regulate the activity of GTPase.…”

Section: Discussionmentioning

confidence: 99%

Genetic variations of the bovine MX1 and their association with mastitis

Chen¹,

Wang²,

Yu³

et al. 2017

Czech J. Anim. Sci.

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The primary agent of mastitis is a wide spectrum of bacterial strains; however, viral-related mastitis has also been reported. The MX dynamin-like GTPase 1 (MX1) gene has been demonstrated to confer positive antiviral responses to many viruses, and may be a suitable candidate gene for the study of disease resistance in dairy cattle. The present study was conducted to investigate the genetic diversity of the MX1 gene in Chinese cattle breeds and its effects on mastitis in Holstein cows. First, polymorphisms were identified in the complete coding region of the bovine MX1 gene in 14 Chinese cattle breeds. An association study was then carried out, utilizing polymorphisms detected in Holstein cows to determine the associations of these single nucleotide polymorphisms (SNPs) with mastitis. We identified 13 previously reported SNPs in Chinese domestic cattle and four of them in Holstein cattle. A novel 12 bp indel was also discovered in Holstein cattle. In addition, haplotype frequencies and linkage disequilibrium of four SNPs detected in Holstein cows were investigated. Analysis of these four SNPs in Chinese Holstein cows revealed two SNPs (g.143181370 T>C and g.143182088 C>T)<br /> significantly (P < 0.05) associated with somatic cell score (SCS). The results indicated that SNPs in the MX1 gene might contribute to the variations observed in the SCS of dairy cattle. Therefore, implementation of these two mutations in selection indexes of the dairy industry might be beneficial by favouring the selection individuals with lower SCS.

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Section: Discussionmentioning

confidence: 99%

Genetic variations of the bovine MX1 and their association with mastitis

Chen¹,

Wang²,

Yu³

et al. 2017

Czech J. Anim. Sci.

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“…Recently this SNP have been demonstrated to confer more resistance in chicken to in vivo experimental infection of highly pathogenic avian influenza virus (HPAIV) (Ewald et al, 2011, Wang et al, 2012, Fulton et al, 2014. SNP at 2032 have been analyzed in various chicken breeds (Li et al, 2006, Seyama et al, 2006, Balkisson et al, 2007. However Indian native chicken breeds are yet to be investigated.…”

Section: Introductionmentioning

confidence: 99%

Chicken Mx gene polymorphisms in Indian native chicken breeds and White Leghorn by real time multiplex allele specific PCR

Ramasamy

Reddy

Ravindra

et al. 2017

IJAR

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Chicken Mx protein confers resistance against viral infections including avian influenza virus. Mx protein is one of the strong antiviral proteins induced by interferon system. Different alleles of chicken Mx gene have been shown to vary in their antiviral activity against influenza virus. A non-synonymous mutation at position 2032 of chicken Mx gene responsible for variation of amino acid (Ser to Asn) in Mx protein is responsible for negative to positive antiviral activity of Mx protein.In the present study it was analyzed that SNP at G2032A in chicken Mx gene in two Indian native chicken breeds, Aseel, Kadaknath and compared with White Leghorn layer. A total of 90 samples, including 50 of White Leghorn and 20 each from native chicken breed were screened for SNP at position 2032 of Mx gene by using real time multiplex allele specific PCR. The resistant allele (A) frequency is higher than susceptible allele in all three chicken breeds investigated. Among the breeds, the resistant allele frequency (A) is higher in White Leghorn (0.95) followed by Aseel (0.75) and Kadaknath (0.625). Homozygous to resistant allele (AA) is the major genotype in White Leghorn whereas heterozygous to both alleles is the major genotype in Kadaknath. Variations in the gene frequency might have emerged from varying degree of selection pressure the different breeds had undergone. In future, the resistant genotype could be selected in modern breeding programs to improve the genetic status for disease resistance against viral infections.

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“…Several studies have investigated the allele frequency of the Mx 2032 G/A polymorphism in the chicken using a variety of methods: mismatched polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) (Seyama et al, 2006), electrophoresis of PCR products on polyacrylamide gels (Li et al, 2006), and sequencing (Balkissoon et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

“…New primers and PCR-RFLP protocol were established. The latter PCR-RFLP-based genotyping protocol that does not require the creation of mismatches (Seyama et al, 2006) is here described.…”

Section: Introductionmentioning

confidence: 99%