Population Genomics Reveals Population Structure and Mating-Type Loci in Marssonina brunnea

Cheng, Qiang; Yang, Hougang; Chen, Junxiang; Zhao, Luo

doi:10.3390/jof8060579

Cited by 3 publications

(3 citation statements)

References 30 publications

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“…With the advent of molecular genomic tools and advanced analytical approaches for population surveillance, a detailed insight into the genomic structure of both pathogens and their hosts can now be achieved. This allows inference of key factors that may affect population genomic structures like host‐pathogen interaction and co‐evolution, geographic and temporal dynamics as well as life history traits (Allen et al, 2018; Blasco‐Costa & Poulin, 2013; Cheng et al, 2022; Mei et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Clonal genomic population structure of Beauveria brongniartii and Beauveria pseudobassiana: Pathogens of the common European cockchafer (Melolontha melolontha L.)

Pedrazzini,

Rehner,

Strasser

et al. 2024

Environmental Microbiology

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Beauveria brongniartii is a fungal pathogen that infects the beetle Melolontha melolontha, a significant agricultural pest in Europe. While research has primarily focused on the use of B. brongniartii for controlling M. melolontha, the genomic structure of the B. brongniartii population remains unknown. This includes whether its structure is influenced by its interaction with M. melolontha, the timing of beetle‐swarming flights, geographical factors, or reproductive mode. To address this, we analysed genome‐wide SNPs to infer the population genomics of Beauveria spp., which were isolated from infected M. melolontha adults in an Alpine region. Surprisingly, only one‐third of the isolates were identified as B. brongniartii, while two‐thirds were distributed among cryptic taxa within B. pseudobassiana, a fungal species not previously recognized as a pathogen of M. melolontha. Given the prevalence of B. pseudobassiana, we conducted analyses on both species. We found no spatial or temporal genomic patterns within either species and no correlation with the population structure of M. melolontha, suggesting that the dispersal of the fungi is independent of the beetle. Both species exhibited clonal population structures, with B. brongniartii fixed for one mating type and B. pseudobassiana displaying both mating types. This implies that factors other than mating compatibility limit sexual reproduction. We conclude that the population genomic structure of Beauveria spp. is primarily influenced by predominant asexual reproduction and dispersal.

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Section: Introductionmentioning

confidence: 99%

Clonal genomic population structure of Beauveria brongniartii and Beauveria pseudobassiana: Pathogens of the common European cockchafer (Melolontha melolontha L.)

Pedrazzini,

Rehner,

Strasser

et al. 2024

Environmental Microbiology

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“…brunnea f. sp. monogermtubi (MbMo) and E. australis (Ea) are common fungal pathogens of Shanxin, and the genomes of these two fungi have been sequenced in our previous studies (Cheng et al, 2022;Zhao et al, 2020) S1). Sanger sequencing showed that the predicted introns were appropriately spliced, and the cloned sequences were all consistent with predicted gene models, indicating that the four NLP genes were not pseudogenes and were expressed in the early stage of infection.…”

mentioning

confidence: 99%

“…M. brunnea f. sp. monogermtubi (MbMo) and E. australis (Ea) are common fungal pathogens of Shanxin, and the genomes of these two fungi have been sequenced in our previous studies (Cheng et al, 2022 ; Zhao et al, 2020 ). A tBLASTn search with NLP of P. parasitica (GenBank accession no.…”

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confidence: 99%

Heterologous expression of Arabidopsis pattern recognition receptor RLP23 increases broad‐spectrum resistance in poplar to fungal pathogens

Zhao

Cheng

2022

Molecular Plant Pathology

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The pattern recognition receptor AtRLP23 from Arabidopsis thaliana recognizes the epitopes (nlp24s) of necrosis and ethylene‐inducing peptide 1‐like proteins (NLPs) and triggers pattern‐triggered immunity (PTI). Here, we established methods for studying the early events of PTI in the hybrid poplar cultivar Shanxin (Populus davidiana × Populus bolleana) in response to the flagellin epitope. We confirmed that wild‐type Shanxin cannot generate PTI responses on nlp24 treatment. Four NLP homologues were characterized from two common fungal pathogens of Shanxin, namely Marssonina brunnea f. sp. monogermtubi (MbMo) and Elsinoë australis (Ea), which cause black leaf spot and anthracnose disease, respectively, and the nlp24s of three of them could be responded to by Nicotiana benthamiana leaves expressing AtRLP23. We then created AtRLP23 transgenic Shanxin lines and confirmed that the heterologous expression of AtRLP23 conferred on transgenic Shanxin the ability to respond to one nlp24 of each fungal pathogen. Consistently, infection assays with MbMo or Ea showed obviously lower levels of disease symptoms and significantly inhibited the growth of fungi on the transgenic poplar compared with that in wild‐type poplar. Overall, our results indicated that the heterologous expression of AtRLP23 allowed transgenic Shanxin to generate a PTI response to nlp24s, resulting in increased broad‐spectrum fungal disease resistance.

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Trait Assessment of 1122 Populus deltoides Clones: Unveiling Correlations among Growth, Wood Properties, and Disease Susceptibility

Ma,

Hou

2024

Forests

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This study aimed to evaluate the growth, wood properties, disease susceptibility, and sex traits of 1122 Populus deltoides clones to reveal the trait variability and correlations, providing a basis for genetic improvement and breeding. The measurements included the diameter at breast height (DBH), leaf area, basic wood density (BWD), content of cellulose, hemicellulose, lignin, and disease susceptibility index (DSI). The coefficients of variation ranged 6.91%–41.96%, with the BWD showing the lowest variability. Significant sexual dimorphism was observed, with male clones exhibiting higher DBH and hemicellulose content, and female clones displaying larger leaf areas and greater phenotypic variability. Correlation analysis revealed that the leaf area was positively correlated with the BWD and hemicellulose, and it was negatively correlated with the DBH and lignin; lignin was negatively correlated with cellulose. PCA confirmed these relationships and additionally highlighted a positive correlation between the DSI and DBH. These findings established links between the growth traits and wood properties, enhancing our understanding of trait diversity in P. deltoides and providing insights for breeding strategies to develop high-quality, high-yielding cultivars.

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Population Genomics Reveals Population Structure and Mating-Type Loci in Marssonina brunnea

Cited by 3 publications

References 30 publications

Clonal genomic population structure of Beauveria brongniartii and Beauveria pseudobassiana: Pathogens of the common European cockchafer (Melolontha melolontha L.)

Clonal genomic population structure of Beauveria brongniartii and Beauveria pseudobassiana: Pathogens of the common European cockchafer (Melolontha melolontha L.)

Heterologous expression of Arabidopsis pattern recognition receptor RLP23 increases broad‐spectrum resistance in poplar to fungal pathogens

Trait Assessment of 1122 Populus deltoides Clones: Unveiling Correlations among Growth, Wood Properties, and Disease Susceptibility

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