Population genomics reveals meiotic recombination in
            <i>Fusarium oxysporum</i>

Fokkens, Like; Rep, Martijn

doi:10.1073/pnas.2309677120

Cited by 2 publications

(1 citation statement)

References 20 publications

(31 reference statements)

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“…In the case of V. dahliae, sexual reproduction is apparently prevented by karyotype variation that interferes with homologous chromosome pairing during meiosis [56]. Nevertheless, strains of F. oxysporum and V. dahliae harbor either the MAT1-1 or MAT1-2 versions of the MAT1 locus [57,58] and not both as is the case for homothallic species such as F. graminearum. The F. oxysporum strain in which chemotropism was studied carries the MAT1-1 version of the locus, while the V. dahliae strain studied carries the MAT1-2 version [15,59].…”

Section: Discussionmentioning

confidence: 99%

Sex Pheromone Receptor Ste2 Orchestrates Chemotropic Growth towards Pine Root Extracts in the Pitch Canker Pathogen Fusarium circinatum

Ramaswe,

Steenkamp,

De Vos

et al. 2024

Pathogens

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In ascomycetous fungi, sexual mate recognition requires interaction of the Ste2 receptor protein produced by one partner with the α-factor peptide pheromone produced by the other partner. In some fungi, Ste2 is further needed for chemotropism towards plant roots to allow for subsequent infection and colonization. Here, we investigated whether this is also true for the pine pitch canker fungus, Fusarium circinatum, which is a devastating pathogen of pine globally. Ste2 knockout mutants were generated for two opposite mating-type isolates, after which all strains were subjected to chemotropism assays involving exudates from pine seedling roots and synthetic α-factor pheromone, as well as a range of other compounds for comparison. Our data show that Ste2 is not required for chemotropism towards any of these other compounds, but, in both wild-type strains, Ste2 deletion resulted in the loss of chemotropism towards pine root exudate. Also, irrespective of mating type, both wild-type strains displayed positive chemotropism towards α-factor pheromone, which was substantially reduced in the deletion mutants and not the complementation mutants. Taken together, these findings suggest that Ste2 likely has a key role during the infection of pine roots in production nurseries. Our study also provides a strong foundation for exploring the role of self-produced and mate-produced α-factor pheromone in the growth and overall biology of the pitch canker pathogen.

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