POPULATION GENOMIC ANALYSIS OFCRYPTOCOCCUSBRAZILIAN ISOLATES REVEALS AN AFRICAN TYPE SUBCLADE DISTRIBUTION

Abstract

The genome of a large number of Cryptococcus neoformans isolates has been sequenced and analyzed in recent years. These genomes have been used to understand the global population structure of this opportunistic pathogen. However, only a small number of South American isolates have been considered in these studies, and the population structure of C. neoformans in this part of the world remains evasive. Here, we analyzed the genomic sequences of 53 Brazilian Cryptococcus isolates and deciphered the C. neoformans population structure in this country. Our data reveal an African-like structure that suggesting repeated and privileged intercontinental transports from Africa to South America. We also identified a mutator phenotype in one VNBII Brazilian isolate, exemplifying how fast-evolving isolates can shape the Cryptococcus population structure. Finally, phenotypic analyses revealed wide diversity but not lineage specificity in the expression of classical virulence traits within the set of isolates.

Coregulation of extracellular vesicle production and fluconazole susceptibility inCryptococcus neoformans

“…DNA read alignment, variant detection, and ploidy analysis were performed as previously described 47 . Illumina reads were aligned to the C. neoformans H99 reference genome using Minimap2 aligner v.2.9 48 with the “-ax sr” parameter.…”