Population genetics of the widespread shrub Forsythia suspensa (Oleaceae) in warm-temperate China using microsatellite loci: implication for conservation

Fu, Zizhen; Lei, Yakai; Peng, Dandan; Li, Yong

doi:10.1007/s00606-015-1241-y

Cited by 7 publications

(4 citation statements)

References 22 publications

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“…The identification of population genetic structure at the genome level can help provide a comprehensive understanding of the effects of these factors on population genetic structure. Recently, three studies have described the population genetic structure of F. suspensa 25,30,55 . The first study of the phylogeography of F. suspensa was based on chloroplast DNA (cpDNA) and nuclear ribosomal DNA (nrDNA).…”

Section: Discussionmentioning

confidence: 99%

Genome sequencing and population genomics modeling provide insights into the local adaptation of weeping forsythia

Cushman

Yan

et al. 2020

Hortic Res

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Understanding the genetic basis underlying the local adaptation of nonmodel species is a fundamental goal in evolutionary biology. In this study, we explored the genetic mechanisms of the local adaptation of Forsythia suspensa using genome sequence and population genomics data obtained from specific-locus amplified fragment sequencing. We assembled a high-quality reference genome of weeping forsythia (Scaffold N50 = 7.3 Mb) using ultralong Nanopore reads. Then, genome-wide comparative analysis was performed for 15 natural populations of weeping forsythia across its current distribution range. Our results revealed that candidate genes associated with local adaptation are functionally correlated with solar radiation, temperature and water variables across heterogeneous environmental scenarios. In particular, solar radiation during the period of fruit development and seed drying after ripening, cold, and drought significantly contributed to the adaptive differentiation of F. suspensa. Natural selection exerted by environmental factors contributed substantially to the population genetic structure of F. suspensa. Our results supported the hypothesis that adaptive differentiation should be highly pronounced in the genes involved in signal crosstalk between different environmental variables. Our population genomics study of F. suspensa provides insights into the fundamental genetic mechanisms of the local adaptation of plant species to climatic gradients.

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Section: Discussionmentioning

confidence: 99%

Genome sequencing and population genomics modeling provide insights into the local adaptation of weeping forsythia

Cushman

Yan

et al. 2020

Hortic Res

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“…1 ). Based on microsatellite loci ( A R = 4.524, H E = 0.563; Table 1 ), the species showed a moderate degree of genetic diversity in comparison with other warm temperate plants of East Asia, such as species of Castanopsis ( Aoki et al 2014 ), Forsythia suspensa ( Fu et al 2016 ), Kirengeshoma palmata ( Yuan et al 2014 ) and Achyranthes bidentata ( Yan et al 2016 ). More common and widespread species have a high genetic diversity in comparison with species endemic to a narrow region, which are prone to harbouring low genetic diversity, probably due to the influence of genetic drift or founder events ( Frankham 1997 ; Cole 2003 ).…”

Section: Discussionmentioning

confidence: 99%

Historical migration and taxonomic entity of Korean endemic shrubLespedeza maritima(Fabaceae) based on microsatellite loci

2021

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Various plant species are endemic to the Korean Peninsula, but their evolutionary divergence and establishment are poorly understood. One of these, Lespedeza maritima, has been proposed as either a hybrid (L. cyrtobotrya × L. maximowiczii) or a synonym of L. thunbergii. A distinct taxon, L. uekii, has been proposed for inland populations. We investigated genetic diversity and structure in L. maritima and related taxa to resolve this. Genotypes of L. maritima (n = 244, including L. uekii) were determined using 12 microsatellite loci, then compared with those of related species. Genetic diversity within L. maritima was estimated, and Bayesian clustering analysis was used to represent its genetic structure and that of related taxa. Its distribution during the Last Glacial Maximum (LGM) was predicted using ecological niche modelling (ENM). Neighbour-joining (NJ) analysis and PCoA were used to investigate relationships among species. Bayesian tree based on nrITS was also reconstructed to show relationships and divergence time among species. Morphological features were examined using flower characteristics. In result, expected heterozygosity (HE) and allelic richness (AR) within L. maritima were higher in southern than northern populations. Bayesian clustering analysis largely assigned populations to two clusters (K = 2) (south vs. north). ENM showed that L. maritima occurred around the East China Sea and Korean Strait land bridge during the LGM. Compared with other Lespedeza species, L. maritima was assigned to an independent cluster (K = 2 to 5), supported by the NJ, PCoA, Bayesian tree and morphological examination results. Lespedeza maritima and L. uekii were clustered to one clade on Bayesian tree. Given results, current L. maritima populations derive from post-LGM colonization away from southern refugia. The type L. uekii, which grows inland, is thought synonym of L. maritima. In addition, L. maritima is considered a distinct species, compared with related taxa.

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“…F. suspensa blooms in early spring, with flowers first and leaves later. F. suspensa trees are golden during flowering period, which confers the plants with excellent ornamental effects ( Fu et al, 2014 ). On the other hand, F. suspensa fruits contain phillyrin, phillyrin A, α-pinene, β-pinene, terpinen-4-ol, and other volatile components, and it is widely used as a Chinese patent medicine for treatment of colds ( Xiang et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Genome-wide analysis of the carotenoid cleavage dioxygenases gene family in Forsythia suspensa: Expression profile and cold and drought stress responses

et al. 2022

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Forsythia suspensa is a famous ornamental and medicinal plant in Oleaceae. CCD family is involved in the synthesis of pigments, volatiles, strigolactones, and abscisic acid (ABA) in plants. In this study, the CCD family in F. suspensa was analyzed at the genome level. A total of 16 members of the CCD family were identified, which included 11 members of the carotenoid cleavage dioxygenases (CCD) subfamily and 5 members of the 9-cis epoxycarotenoid dioxygenases (NCED) subfamily. The expression analysis of different tissues demonstrated that three FsCCD1 genes might be involved in the synthesis of pigments and volatiles in flowers and fruits. Three CCD4 genes were effectively expressed in flowers, while only FsCCD4-3 was effectively expressed in fruits. Comparison of CCD4 between Osmanthus fragrans and F. suspensa showed that the structure of FsCCD4-1 is was comparable that of OfCCD4-1 protein, indicating that the protein might be performing, especially in catalyzing the synthesis of β-ionone. However, further comparison of the upstream promoter regions showed that the proteins have major differences in the composition of cis-elements, which might be responsible for differences in β-ionone content. On the other hand, four NCED genes were significantly up-regulated under cold stress while two were up-regulated in drought stress. The data showed that these genes might be involved in the synthesis of ABA. Taken together, our data improves understanding of the CCD family and provides key candidate genes associated with cold and drought stresses in F. suspensa.

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Population genetics of the widespread shrub Forsythia suspensa (Oleaceae) in warm-temperate China using microsatellite loci: implication for conservation

Cited by 7 publications

References 22 publications

Genome sequencing and population genomics modeling provide insights into the local adaptation of weeping forsythia

Genome sequencing and population genomics modeling provide insights into the local adaptation of weeping forsythia

Historical migration and taxonomic entity of Korean endemic shrubLespedeza maritima(Fabaceae) based on microsatellite loci

Genome-wide analysis of the carotenoid cleavage dioxygenases gene family in Forsythia suspensa: Expression profile and cold and drought stress responses

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