Population genetics of Philotheca sporadica (Rutaceae) to advise an offset translocation program

Shapcott, Alison; Lamont, Robert W.; O’Connor, Katie; James, Heather E.; Conroy, Gabriel

doi:10.1007/s10592-014-0693-x

Cited by 10 publications

(2 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Population size has a significant effect on genetic diversity [ 33 ]. Generally, smaller population size leads to lower genetic diversity [ 34 ]. Extinction and contraction of species’ distribution during successive ice ages has resulted in reduced population size and resultant diversity bottlenecks in other Australian flora, such as Acacia, Banksia, Eucalyptus etc.…”

Section: Discussionmentioning

confidence: 99%

Genetic Structure of Wild Germplasm of Macadamia: Species Assignment, Diversity and Phylogeographic Relationships

Mai

Alam

Hardner

et al. 2020

Plants

View full text Add to dashboard Cite

Macadamia is an Australian native rainforest tree that has been domesticated and traded internationally for its premium nuts. Common cultivars rely upon a limited gene pool that has exploited only two of the four species. Introducing a more diverse germplasm will broaden the genetic base for future crop improvement and better adaptation for changing environments. This study investigated the genetic structure of 302 accessions of wild germplasm using 2872 SNP and 8415 silicoDArT markers. Structure analysis and principal coordinate analysis (PCoA) assigned the 302 accessions into four distinct groups: (i) Macadamia integrifolia, (ii) M. tetraphylla, and (iii) M. jansenii and M. ternifolia, and (iv) admixtures or hybrids. Assignment of the four species matched well with previous characterisations, except for one M. integrifolia and four M. tetraphylla accessions. Using SNP markers, 94 previously unidentified accessions were assigned into the four distinct groups. Finally, 287 accessions were identified as pure examples of one of the four species and 15 as hybrids of M. integrifolia and M. tetraphylla. The admixed accessions showed the highest genetic diversity followed by M. integrifolia, while M. ternifolia and M. jansenii accessions were the least diverse. Mantel test analysis showed a significant correlation between genetic and geographic distance for M. integrifolia (r = 0.51, p = 0.05) and a positive but not significant correlation for M. tetraphylla (r = 0.45, p = 0.06). This study provides a population genetics overview of macadamia germplasm as a background for a conservation strategy and provides directions for future macadamia breeding.

show abstract

Section: Discussionmentioning

confidence: 99%

Genetic Structure of Wild Germplasm of Macadamia: Species Assignment, Diversity and Phylogeographic Relationships

Mai

Alam

Hardner

et al. 2020

Plants

View full text Add to dashboard Cite

show abstract

“…Approximately 30–50 mg of dried plant tissue and a 3‐mm tungsten bead were frozen using liquid nitrogen for 30 s and ground using a Retsch MM200 Tissue Lyser grinding mill (QIAGEN, Valencia, California, USA). Total genomic DNA was extracted from the leaf tissue using QIAGEN DNeasy Plant Mini Kits (QIAGEN) following manufacturer's instructions as described in Shapcott et al (2015). DNA (5 µg) from four individuals was sent to the Australian Genome Research Facility (AGRF, Brisbane, Australia; http://agrf.org.au/) for next‐generation 454 pyrosequencing and used to construct a random library that was paired‐end sequenced using GS‐FLX Titanium chemistry Illumina HiSeq (Roche Applied Science, Mannheim, Germany).…”

Section: Methods and Resultsmentioning

confidence: 99%

Characterization of Microsatellite Primers in the Endangered Orchid Phaius australis and Cross‐Amplification to P. bernaysii (Orchidaceae)

2017

Self Cite

View full text Add to dashboard Cite

Premise of the study:The swamp orchid, Phaius australis (Orchidaceae), is nationally endangered due to illegal collection and habitat loss and fragmentation, resulting in a disjunct distribution in spring and coastal wetland ecotones along Australia’s east coast. Polymorphic microsatellite markers were developed to study genetic diversity and population structure for conservation and restoration purposes.Methods and Results:Illumina HiSeq high-throughput sequencing was used to develop 15 nuclear microsatellite markers, including 10 polymorphic markers for P. australis. Polymorphism at each marker was evaluated using 90 individuals from four natural populations. The number of alleles per locus ranged from one to three, and the observed and expected heterozygosity varied from 0.036 to 0.944 and from 0.035 to 0.611, respectively. These markers transferred successfully to congener P. bernaysii.Conclusions:The microsatellite markers will be useful for revealing levels of genetic diversity and gene flow for P. australis and may inform future conservation efforts.

show abstract

Genetics and species distribution modelling of Solanum johnsonianum (Solanaceae) reveal impacts of brigalow land clearing on this endemic species

et al. 2017

View full text Add to dashboard Cite

Population genetics of Philotheca sporadica (Rutaceae) to advise an offset translocation program

Cited by 10 publications

References 48 publications

Genetic Structure of Wild Germplasm of Macadamia: Species Assignment, Diversity and Phylogeographic Relationships

Genetic Structure of Wild Germplasm of Macadamia: Species Assignment, Diversity and Phylogeographic Relationships

Characterization of Microsatellite Primers in the Endangered Orchid Phaius australis and Cross‐Amplification to P. bernaysii (Orchidaceae)

Genetics and species distribution modelling of Solanum johnsonianum (Solanaceae) reveal impacts of brigalow land clearing on this endemic species

Contact Info

Product

Resources

About