Population genetics and conservation of the extremely narrow Pyrenean palaeoendemic<i>Glandora oleifolia</i>(Boraginaceae)

Hoyo, Alberto del; López‐Pujol, Jordi; Chung, Mi Yoon; Vega, Blanca Lasso de la

doi:10.1080/17550874.2012.735270

Cited by 6 publications

(3 citation statements)

References 63 publications

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“…The average percentage of polymorphic loci (75–85%) ( Table 3 ) and total gene diversity (0.293) were in the range previously reported with RAPDs for other endemic or endangered species [17] , [29] , [30] , [31] ; in contrast, percent polymorphism was higher in subsp. moggridgei than in the Iranian endemic F. imperialis L. a much more widespread congeneric [32] .…”

Section: Discussionsupporting

confidence: 71%

“…The latter can be considered a measure of phenotypic diversity (I = −Σp i log 2 p i , where p i is the frequency of presence or absence of a given RAPD fragment; [16] ), assuming that populations are not in Hardy-Weinberg equilibrium. This index is frequently used in RAPD analysis because it is insensitive to bias that may be introduced into data owing to undetectable heterozygosity [17] . Calculations were performed using the GENALEX 6.5 software package [18] .…”

Section: Methodsmentioning

confidence: 99%

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Genetic Variability and Population Divergence in the Rare Fritillaria tubiformis subsp. moggridgei Rix (Liliaceae) as Revealed by RAPD Analysis

2014

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Fritillaria tubiformis subsp. moggridgei Rix. is a rare alpine geophyte with shiny yellow flowers. This plant is sporadically distributed across the southwestern Alps where it is biogeographically close to F. tubiformis var. burnatii Planch. The latter has dark purple flowers and ranges in the majority of the Western and Central Alps. In order to develop appropriate strategies of conservation, a RAPD based analysis was conducted to study the genetic status of these taxa and the distribution of genetic variability of the subspecies by sampling seven populations distributed across the subspecies' range. Four populations of var. burnatii were chosen within this range and included in the genetic analysis. Some 264 individuals were analysed and 201 polymorphic loci were scored. Genetic diversity scored in the subspecies was in line with expectations for endemic species (He = 0.194). F. tubiformis var. burnatii showed lower intraspecific diversity (He = 0.173), notwithstanding a wider range than the subspecies. Most of the total phenotypic variation (about 83%) was allocated within populations, and significant lower proportions between taxa (6.45%) and between populations of the same taxon (10.64%). Moreover, PCoA analysis and Bayesian clustering separated populations into two genetically differentiated groups corresponding with the subspecific taxa. However, three populations ascribed to the subsp. moggridgei repeatedly showed genetic admixture with var. burnatii populations. Our findings suggest that: i) although the different flower colour, the two taxa are genetically very similar and share a consistent part of their gene pool, ii) the majority of genetic variability is allocated within populations rather than among them, iii) a representative amount of genetic diversity can be preserved by sampling from a restricted number of populations. The efficacy of RAPD markers in analysing genetic variation, and the contribution of the results to the preservation of biodiversity of the species, are discussed.

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Section: Discussionsupporting

confidence: 71%

Section: Methodsmentioning

confidence: 99%

Genetic Variability and Population Divergence in the Rare Fritillaria tubiformis subsp. moggridgei Rix (Liliaceae) as Revealed by RAPD Analysis

2014

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“…The limitation of low reproducibility and dominant nature in early RAPD can now be overcome through improved laboratory techniques and band scoring procedures, and analysis of molecular variance (AMOVA) [12][13][14][15]. Although RAPD analysis has some disadvantages, it is a useful technique for investigating population genetic diversity and has been applied to many rare plants [16,17]. The aim of this study was to use RAPD markers to characterize genetic diversity in H. microcephala, to investigate the genetic variation and to propose conservation strategies for H. microcephala.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and population structure of the narrow endemic and endangered species Heteroplexis microcephala Y. L. Chen. in China revealed by random amplified polymorphic DNA markers

Shi

Xiao

JiQing

et al. 2016

ARCH BIOL SCI BELGRA

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Heteroplexis microcephala Y. L. Chen. is an endemic and endangered species found only in karst limestone regions in the Yangshuo County of the Guangxi Zhuang Autonomous Region in China: it is a habitat representative of species in the Heteroplexis genus. To provide basic genetic information for its conservation, in this study we evaluated the genetic variation and differentiation among six wild populations of H. microcephala by random amplified polymorphic DNA markers (RAPD). The leaves of 141 individuals were sampled. Based on 12 primers, 113 DNA fragments were generated. Genetic diversity was low at the population level (Nei's gene diversity (h)=0.0579; Shannon information index (I)=0.0924; percentage of polymorphic bands (PPB)=23.30%), but relatively high at the species level (h=0.1701; I=0.2551; PPB=46.34%). The coefficient of genetic differentiation based on Nei's genetic diversity analysis (0.6661) was high, indicating that there was significant genetic differentiation among populations, which was confirmed by AMOVA analysis exhibiting population differentiation among populations of 68.77%. Low gene flow among populations (0.2507) may result from several factors, such as a harsh pollination environment, population isolation and low seed dispersal distance. Limited gene flow and self-compatibility are the primary reasons for the high genetic differentiation observed for this species. We propose the collection of seeds from more populations with fewer individuals and core populations for ex situ conservation and suggest methods to increase seed germination rates.

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Genetic diversity and structure of Drimys brasiliensis in southern Brazil: insights for conservation

2019

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Population genetics and conservation of the extremely narrow Pyrenean palaeoendemicGlandora oleifolia(Boraginaceae)

Cited by 6 publications

References 63 publications

Genetic Variability and Population Divergence in the Rare Fritillaria tubiformis subsp. moggridgei Rix (Liliaceae) as Revealed by RAPD Analysis

Genetic Variability and Population Divergence in the Rare Fritillaria tubiformis subsp. moggridgei Rix (Liliaceae) as Revealed by RAPD Analysis

Genetic diversity and population structure of the narrow endemic and endangered species Heteroplexis microcephala Y. L. Chen. in China revealed by random amplified polymorphic DNA markers

Genetic diversity and structure of Drimys brasiliensis in southern Brazil: insights for conservation

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