Population genetic structure of the parasitic nematode Camallanus cotti inferred from DNA sequences of ITS1 rDNA and the mitochondrial COI gene

Wu, Shan; Wang, Gui T.; Xi, Bingwen; Xiong, Fan; Liu, Tao; Nie, Pin

doi:10.1016/j.vetpar.2009.04.030

Cited by 31 publications

(29 citation statements)

References 35 publications

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“…All genomic DNA was stored at −20°C. Approximately 420 bp of mitochondrial cytochrome oxidase I gene (COI) were amplified in all specimens using the primers FCOX1A and RCOX1A of Wu et al [29]. A subset of these specimens was utilized for phylogenetic analyses.…”

Section: Methodsmentioning

confidence: 99%

An Invasive Fish and the Time-Lagged Spread of Its Parasite across the Hawaiian Archipelago

et al. 2013

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Efforts to limit the impact of invasive species are frustrated by the cryptogenic status of a large proportion of those species. Half a century ago, the state of Hawai'i introduced the Bluestripe Snapper, Lutjanus kasmira, to O'ahu for fisheries enhancement. Today, this species shares an intestinal nematode parasite, Spirocamallanus istiblenni, with native Hawaiian fishes, raising the possibility that the introduced fish carried a parasite that has since spread to naïve local hosts. Here, we employ a multidisciplinary approach, combining molecular, historical, and ecological data to confirm the alien status of S. istiblenni in Hawai'i. Using molecular sequence data we show that S. istiblenni from Hawai'i are genetically affiliated with source populations in French Polynesia, and not parasites at a geographically intermediate location in the Line Islands. S. istiblenni from Hawai'i are a genetic subset of the more diverse source populations, indicating a bottleneck at introduction. Ecological surveys indicate that the parasite has found suitable intermediate hosts in Hawai'i, which are required for the completion of its life cycle, and that the parasite is twice as prevalent in Hawaiian Bluestripe Snappers as in source populations. While the introduced snapper has spread across the entire 2600 km archipelago to Kure Atoll, the introduced parasite has spread only half that distance. However, the parasite faces no apparent impediments to invading the entire archipelago, with unknown implications for naïve indigenous Hawaiian fishes and the protected Papahānaumokuākea Marine National Monument.

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Section: Methodsmentioning

confidence: 99%

An Invasive Fish and the Time-Lagged Spread of Its Parasite across the Hawaiian Archipelago

et al. 2013

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“…Significant between-host differences are frequently explained as a result of clumped transmission and small infrapopulation sizes resulting in little gene flow and strong drift (Nadler, 1995;Criscione et al, 2010), whereas small differences are associated with larger infrapopulations (Sire et al, 2001;Theron et al, 2004;Johnson et al, 2006). Irrespective of the between host differences, it is possible to find no genetic structure over vast geographic distances (Jefferies et al, 2010;Baldwin et al, 2011) or significant structure (Wu et al, 2009;Belanger et al, 2011), in some populations, even at a local scale (Criscione et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

High within-host genetic variation of the nematode Spirocerca lupi in a high-density urban dog population

Waal

Gous

Clift

et al. 2012

Veterinary Parasitology

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The nematode worm Spirocerca lupi has a cosmopolitan distribution and can cause the death of its final canid host, typically dogs. While its life cycle, which involves a coprophagous beetle intermediate host, a number of non-obligatory vertebrate paratenic hosts and a canid final host, is well understood, surprisingly little is known about its transmission dynamics and population genetic structure. Here we sequenced cox1 to quantify genetic variation and the factors that limit gene flow in a 300km 2 area in South Africa. Three quarters of the genetic variation, was explained by differences between worms from the same host, whereas a quarter of the variation was explained by differences between worms from different hosts. With the help of a newly derived model we conclude that while the offspring from different infrapopulations mixes fairly frequently in new hosts, the level of admixture is not enough to homogenize the parasite populations among dogs. Small infrapopulation sizes along with clumped transmission may also result in members of infrapopulations being closely related.

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“…Comparison of the two markers indicated that haplotype and nucleotide diversity were higher from mtDNA marker than that from nuclear marker. This is presumably because mtDNA is haploid and maternally inherited, therefore the effective population size of mtDNA is a quarter that of nuclear genes, suggesting COI is substantially more differentiated than ITS2 rDNA in these populations (Ballard and Whitlock, 2004;Wu et al, 2009). The mean haplotype diversity values of COI and ITS2 rDNA markers were 0.986 ± 0.006 and 0.955 ± 0.008, respectively, indicating a high genetic variability.…”

Section: Discussionmentioning

confidence: 97%

“…Nuclear and mitochondrial DNA markers have been used for species identification and genetic diversity studies of nematodes (Itagaki et al, 2005;Loaizaa et al, 2013;Wu et al, 2009). Mitochondrial genes are used for investigating population structure, deciphering phylogeography, and phylogenetic relationships (Xu et al, 2009) due to the advantages associated with maternal inheritance, lack of recombination or rapid evolution, and availability of PCR primers and genome sequences of a wide range of species (Chang et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

Populations genetic structure of the razor clam Sinonovacula constricta from China, Korea and Vietnam

Tran

Niu

Nguyen

et al. 2015

Biochemical Systematics and Ecology

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Population genetic structure of the parasitic nematode Camallanus cotti inferred from DNA sequences of ITS1 rDNA and the mitochondrial COI gene

Cited by 31 publications

References 35 publications

An Invasive Fish and the Time-Lagged Spread of Its Parasite across the Hawaiian Archipelago

An Invasive Fish and the Time-Lagged Spread of Its Parasite across the Hawaiian Archipelago

High within-host genetic variation of the nematode Spirocerca lupi in a high-density urban dog population

Populations genetic structure of the razor clam Sinonovacula constricta from China, Korea and Vietnam

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